##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545992_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	844016
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.90882992739474	31.0	28.0	33.0	23.0	33.0
2	28.634792468389225	31.0	28.0	33.0	16.0	33.0
3	28.120963346666414	31.0	26.0	33.0	16.0	34.0
4	32.13524151200925	35.0	32.0	35.0	25.0	37.0
5	33.63620950313738	35.0	33.0	35.0	28.0	37.0
6	33.463740023885805	35.0	33.0	37.0	28.0	37.0
7	34.423837936721576	35.0	35.0	37.0	32.0	37.0
8	34.7570437053326	35.0	35.0	37.0	32.0	37.0
9	36.599483896039885	39.0	37.0	39.0	32.0	39.0
10	36.23461285094121	38.0	35.0	39.0	31.0	39.0
11	36.31021449830335	38.0	35.0	39.0	32.0	39.0
12	34.971595325207105	37.0	33.0	39.0	28.0	39.0
13	35.17736156660537	37.0	34.0	39.0	27.0	39.0
14	35.85203361073724	38.0	34.0	40.0	27.0	41.0
15	36.5227009914504	38.0	35.0	40.0	31.0	41.0
16	36.15589633371879	38.0	34.0	40.0	31.0	41.0
17	35.75418238516805	37.0	34.0	40.0	30.0	41.0
18	35.421386561392204	37.0	34.0	39.0	29.0	40.0
19	34.90930740649466	37.0	33.0	39.0	27.0	40.0
20	35.26322131334003	35.0	34.0	39.0	29.0	40.0
21	35.38651992379291	36.0	34.0	39.0	30.0	40.0
22	35.80475962540994	36.0	34.0	40.0	31.0	41.0
23	36.19877348296715	37.0	35.0	40.0	32.0	41.0
24	35.722184176603285	36.0	34.0	40.0	31.0	41.0
25	35.352635495061705	36.0	34.0	40.0	30.0	41.0
26	35.5387326780535	36.0	34.0	40.0	30.0	41.0
27	35.88603296619969	37.0	35.0	40.0	31.0	41.0
28	35.82544288259938	36.0	35.0	40.0	31.0	41.0
29	36.00175352126026	37.0	35.0	40.0	31.0	41.0
30	35.668207711702145	36.0	35.0	40.0	30.0	41.0
31	35.004701332676156	36.0	34.0	40.0	29.0	41.0
32	34.98559505980929	36.0	34.0	40.0	29.0	41.0
33	34.70451389547117	36.0	34.0	40.0	25.0	41.0
34	34.31113983621164	36.0	34.0	40.0	23.0	41.0
35	34.06626059221626	36.0	34.0	40.0	21.0	41.0
36	33.6434759530625	36.0	33.0	40.0	18.0	41.0
37	33.544893698697656	36.0	33.0	40.0	18.0	41.0
38	33.4204019829008	36.0	33.0	40.0	18.0	41.0
39	33.19277122708574	36.0	33.0	40.0	18.0	41.0
40	32.99159494606737	35.0	33.0	40.0	17.0	40.0
41	32.689582898902394	35.0	32.0	39.0	15.0	40.0
42	32.851672243180225	35.0	33.0	39.0	15.0	40.0
43	32.65121395802923	35.0	32.0	39.0	15.0	40.0
44	32.37529501810392	35.0	32.0	39.0	13.0	40.0
45	32.335326581486605	35.0	32.0	39.0	14.0	40.0
46	32.17520520937992	35.0	31.0	39.0	14.0	40.0
47	32.159238687418245	35.0	31.0	38.0	14.0	40.0
48	32.20861452863453	35.0	32.0	38.0	15.0	40.0
49	32.251048558321166	35.0	32.0	39.0	14.0	40.0
50	32.421307179010824	35.0	32.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	8.0
14	24.0
15	98.0
16	220.0
17	474.0
18	913.0
19	1587.0
20	2526.0
21	3726.0
22	5260.0
23	7460.0
24	10310.0
25	15770.0
26	22400.0
27	26124.0
28	25322.0
29	24653.0
30	27009.0
31	32801.0
32	43890.0
33	66625.0
34	105725.0
35	78556.0
36	83041.0
37	115666.0
38	121480.0
39	22341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.802553506094675	22.739971754089968	27.302444503421736	22.15503023639362
2	36.58402210384637	20.600912778904664	29.774790999222763	13.040274118026199
3	28.087737673219465	18.92949896684423	39.75505203692821	13.227711323008096
4	24.567899186745276	25.201654944929953	36.044221910485106	14.186223957839662
5	20.434565221512386	29.54884741521488	36.74195749843605	13.274629864836685
6	18.786610680366252	36.7379291387841	34.04781425944532	10.427645921404334
7	76.42343273113306	5.206536368978787	15.697332751985742	2.6726981479024094
8	77.96510966616746	3.672205266250877	15.425063031980438	2.937622035601221
9	74.36351917499195	5.179167219578776	16.811885082747246	3.6454285226820344
10	44.8214251862524	23.18060321131353	21.355282364315368	10.64268923811871
11	38.6387224886732	19.633514056605563	29.25003791397319	12.477725540748043
12	34.64768440408712	20.231843946086332	31.111969441337607	14.008502208488938
13	20.424849765881213	33.560738185058106	32.11432010767568	13.900091941384998
14	15.50124642186878	34.43880210801691	35.54825974863036	14.511691721483952
15	14.766544710052889	21.848045534681805	48.45784914030066	14.927560614964644
16	17.135812591230497	18.601661579875266	46.830865765577904	17.431660063316336
17	17.597770658376145	18.173707607438722	35.354898485336776	28.87362324884836
18	20.947114746639876	20.96500540274118	39.09238687418248	18.995492976436466
19	25.740151845462645	22.503127902788574	32.95909082292279	18.797629428825992
20	26.75020378760592	22.159769483042975	32.59239161342913	18.497635115921973
21	21.381940626717977	25.054145892968855	35.18132357680423	18.38258990350894
22	21.450185778468654	22.02825538852344	33.0943963147618	23.4271625182461
23	18.79123144584937	26.302344979242097	32.66158461450968	22.244838960398855
24	18.650831263862298	21.913565619609106	42.135220185399334	17.300382931129267
25	17.697413319178786	23.102405641599212	39.607779947299576	19.59240109192243
26	17.143158423536995	28.83322117116263	34.30989459915452	19.713725806145852
27	16.985341510113553	28.98985327292374	35.43499175371083	18.58981346325188
28	16.03251597126121	25.575226062065177	41.033463820591074	17.358794146082538
29	16.814136224905692	22.31663854713655	40.74318496331823	20.126040264639535
30	19.21456465280279	26.070714299254988	36.782359576121785	17.93236147182044
31	26.351751625561597	24.053335481791816	31.64691190688328	17.948000985763304
32	26.89380296108131	23.559150537430572	32.09204564842373	17.455000853064398
33	25.30425963488844	24.807231142537585	30.30937802126974	19.57913120130424
34	19.74216128604197	25.2866059411196	32.8305387575591	22.14069401527933
35	19.33600784819245	25.193005819794884	33.84509298401926	21.6258933479934
36	27.55717901082444	24.474062103088094	29.124210915432887	18.844547970654585
37	20.263241455138292	29.521715228147333	30.944200109950522	19.270843206763853
38	21.434546264525792	29.65512502132661	27.951958256715514	20.95837045743209
39	21.323529411764707	28.44590623874429	29.2269340865576	21.003630262933402
40	23.833197474929385	25.222507630187106	28.786776553998976	22.157518340884533
41	19.161366609163807	23.808790354685218	30.12122992929044	26.908613106860535
42	22.57848192451328	24.00570605296582	28.001127940702546	25.414684081818358
43	21.796861670868797	25.001895698659744	28.76319880191845	24.438043828553013
44	20.477218441356563	28.07612651892855	29.554652992360335	21.892002047354552
45	18.446451252108965	32.56182347254081	26.858377092377395	22.133348182972835
46	21.623286762336257	29.21875888608747	28.274937915868893	20.88301643570738
47	20.8228280032606	26.194171674470628	30.102983829690434	22.88001649257834
48	22.0845339424845	24.34598396238934	32.11041022918997	21.459071865936192
49	21.19343709123998	23.855471934181345	32.725327481943474	22.225763492635213
50	19.53718886845747	29.506431157703172	29.893390646622812	21.062989327216545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	267.0
1	587.0
2	907.0
3	11282.5
4	21658.0
5	15324.5
6	8991.0
7	9129.0
8	9267.0
9	9758.0
10	10249.0
11	10313.5
12	10378.0
13	9899.0
14	9420.0
15	8808.0
16	8196.0
17	7705.5
18	7215.0
19	6592.5
20	5970.0
21	5751.0
22	5532.0
23	5433.5
24	5335.0
25	5865.0
26	6395.0
27	7391.5
28	8388.0
29	8852.0
30	9316.0
31	11098.0
32	12880.0
33	14174.0
34	15468.0
35	18419.5
36	21371.0
37	23791.0
38	26211.0
39	32359.5
40	38508.0
41	48973.0
42	59438.0
43	67957.0
44	76476.0
45	76402.5
46	76329.0
47	70778.0
48	65227.0
49	64201.0
50	63175.0
51	59139.0
52	55103.0
53	50652.5
54	46202.0
55	42018.0
56	37834.0
57	33828.5
58	29823.0
59	27182.0
60	24541.0
61	22129.5
62	19718.0
63	17830.0
64	15942.0
65	13145.0
66	10348.0
67	9034.5
68	7721.0
69	6635.5
70	5550.0
71	4511.0
72	3472.0
73	2906.0
74	2340.0
75	1773.5
76	1207.0
77	1015.0
78	823.0
79	611.0
80	399.0
81	302.0
82	205.0
83	156.5
84	108.0
85	86.5
86	65.0
87	47.0
88	29.0
89	19.5
90	10.0
91	7.5
92	5.0
93	3.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	844016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.062807957441663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67685420470802	22.44995926822007
2	7.5846506850333215	4.560317939368702
3	3.517774994891535	3.1726258232674365
4	2.2886506029516385	2.752130542328727
5	1.654887958236334	2.487528943977083
6	1.2787492385875447	2.3065675671229267
7	0.9677766437540039	2.0365858370811836
8	0.8154586797106641	1.96119821482965
9	0.6591644974080861	1.7834702128148563
>10	5.715021139812233	36.27419232999818
>50	0.7675172124286682	15.529021319311825
>100	0.07269961120792331	2.8879176045516965
>500	0.0	0.0
>1k	3.9726563501597416E-4	0.12790867848620263
>5k	0.0	0.0
>10k+	3.9726563501597416E-4	1.6705757186414587
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13988	1.6573145532786109	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1071	0.12689332903641637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028198517563648082	0.0
2	0.0	0.0	0.0	0.09940569847017118	0.0
3	1.1848116623381547E-4	0.0	0.0	0.13803055866239503	0.0
4	1.1848116623381547E-4	0.0	0.0	0.2196640821974939	0.0
5	1.1848116623381547E-4	0.0	0.0	0.3806799871092491	0.0
6	1.1848116623381547E-4	0.0	0.0	0.5951308979924551	0.0
7	1.1848116623381547E-4	0.0	0.0	0.7512890750886239	0.0
8	1.1848116623381547E-4	0.0	0.0	1.2185787947147921	0.0
9	2.3696233246763094E-4	0.0	0.0	1.4574368258421642	0.0
10	2.3696233246763094E-4	0.0	0.0	1.8052975299046463	0.0
11	2.3696233246763094E-4	0.0	0.0	2.018682110291748	0.0
12	2.3696233246763094E-4	0.0	0.0	2.2201000928892345	0.0
13	2.3696233246763094E-4	0.0	0.0	2.3083685617334266	0.0
14	2.3696233246763094E-4	0.0	0.0	2.3388182214555173	0.0
15	2.3696233246763094E-4	0.0	0.0	2.376258269985403	0.0
16	2.3696233246763094E-4	0.0	0.0	2.460379898011412	0.0
17	3.554434987014464E-4	0.0	0.0	2.5661835794582095	0.0
18	3.554434987014464E-4	0.0	0.0	2.727554927868666	0.0
19	4.739246649352619E-4	0.0	0.0	2.7920086822998615	0.0
20	4.739246649352619E-4	0.0	0.0	2.878618414816781	0.0
21	4.739246649352619E-4	0.0	0.0	2.968190176489545	0.0
22	4.739246649352619E-4	0.0	0.0	3.06771435612595	0.0
23	4.739246649352619E-4	0.0	0.0	3.1702005649182006	0.0
24	5.924058311690774E-4	0.0	0.0	3.2502938332922597	0.0
25	5.924058311690774E-4	0.0	0.0	3.3180650603780024	0.0
26	5.924058311690774E-4	0.0	0.0	3.3933006009364752	0.0
27	5.924058311690774E-4	0.0	0.0	3.4647447441754657	0.0
28	5.924058311690774E-4	0.0	0.0	3.539861803567705	0.0
29	5.924058311690774E-4	0.0	0.0	3.6281302724118976	0.0
30	5.924058311690774E-4	0.0	0.0	3.756326894276886	0.0
31	5.924058311690774E-4	0.0	0.0	3.853718412921082	0.0
32	5.924058311690774E-4	0.0	0.0	3.94494891092112	0.0
33	5.924058311690774E-4	0.0	0.0	4.0381935887471325	0.0
34	5.924058311690774E-4	0.0	0.0	4.138428655380941	0.0
35	5.924058311690774E-4	0.0	0.0	4.269350344069307	0.0
36	5.924058311690774E-4	0.0	0.0	4.364253758222593	0.0
37	5.924058311690774E-4	0.0	0.0	4.461526795700555	0.0
38	5.924058311690774E-4	0.0	0.0	4.584747208583724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.855292E-4	44.0	1
CTCACGA	75	0.0	44.0	24
GTCGATC	20	7.855292E-4	44.0	21
CTATCGG	35	1.4454781E-7	44.0	2
CTAAGCG	55	1.8189894E-12	44.0	1
CCGTACT	20	7.855292E-4	44.0	25
TCGTACT	25	4.4418317E-5	44.0	33
CGTAATC	20	7.855292E-4	44.0	32
ACCGTAA	30	2.5269146E-6	44.0	17
ATCGATG	25	4.4418317E-5	44.0	1
AGTCGTT	25	4.4418317E-5	44.0	28
CTTACCG	25	4.4418317E-5	44.0	33
CGTTTTT	9680	0.0	43.295456	1
ATAGGGC	270	0.0	41.555557	4
TGCGAAG	75	0.0	41.066666	1
CGGTCTA	75	0.0	41.066666	31
TACACGG	75	0.0	41.066666	2
GGGCGAT	1030	0.0	39.728157	7
TCGATAG	50	1.3478711E-9	39.600002	1
CGTTGAT	135	0.0	39.111115	25
>>END_MODULE