FastQCFastQC Report
Thu 26 May 2016
SRR1545991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545991_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences830601
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138291.664939001999757No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10430.12557172457052182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTC207.855218E-444.00000434
ACTATCG207.855218E-444.0000041
GTATAAC207.855218E-444.00000428
TTACCGA207.855218E-444.00000437
TAGTACG207.855218E-444.0000041
TTGTACG750.044.01
TACGGGT502.7284841E-1144.04
TCGATAG454.802132E-1044.01
TACTACG351.4454417E-744.01
CGGTCTA700.044.031
CGTTTTT93650.042.9428751
TACGGGA1850.042.8108144
ACTACGG603.6379788E-1240.3333322
GTTTTTT105250.038.9833762
TAAGGGA5950.038.823534
CGACGGT800.038.50000428
GACGGTC800.038.50000429
CGATGAT404.122703E-738.50000410
GGTACCT1950.038.3589748
GGGCCTA1500.038.133337
Produced by FastQC (version 0.11.5)