##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545991_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	830601
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.794336871735045	31.0	28.0	31.0	16.0	33.0
2	28.523109170347738	31.0	28.0	33.0	16.0	33.0
3	28.00743196793647	31.0	26.0	33.0	16.0	34.0
4	32.01781601515047	35.0	32.0	35.0	25.0	37.0
5	33.57541346567124	35.0	33.0	35.0	28.0	37.0
6	33.41411459894703	35.0	33.0	37.0	28.0	37.0
7	34.3475037954445	35.0	35.0	37.0	31.0	37.0
8	34.717651435526804	35.0	35.0	37.0	32.0	37.0
9	36.57658370264423	39.0	37.0	39.0	32.0	39.0
10	36.301049481038426	38.0	35.0	39.0	32.0	39.0
11	36.30779881074066	38.0	35.0	39.0	32.0	39.0
12	35.12648672467286	37.0	34.0	39.0	29.0	39.0
13	35.25496839035831	37.0	34.0	39.0	29.0	39.0
14	35.904729226186824	38.0	34.0	40.0	27.0	41.0
15	36.48266135003449	38.0	35.0	40.0	31.0	41.0
16	36.09718023455305	38.0	34.0	40.0	31.0	41.0
17	35.70033987438012	37.0	34.0	40.0	30.0	41.0
18	35.33913997214066	37.0	33.0	39.0	29.0	40.0
19	34.80764289953901	37.0	33.0	39.0	27.0	40.0
20	35.33849826812152	36.0	34.0	39.0	30.0	40.0
21	35.4478744908807	36.0	34.0	39.0	30.0	40.0
22	35.81196507107504	36.0	34.0	40.0	31.0	41.0
23	36.1804500596556	37.0	35.0	40.0	31.0	41.0
24	35.73592735862346	36.0	34.0	40.0	31.0	41.0
25	35.38331400997591	36.0	34.0	40.0	30.0	41.0
26	35.54963694963045	36.0	34.0	40.0	30.0	41.0
27	35.888723948080965	37.0	35.0	40.0	31.0	41.0
28	35.828449520287116	36.0	35.0	40.0	31.0	41.0
29	35.99384421641679	37.0	35.0	40.0	31.0	41.0
30	35.667179548302975	36.0	35.0	40.0	30.0	41.0
31	34.97806407649401	36.0	34.0	40.0	29.0	41.0
32	34.974203016851654	36.0	34.0	40.0	29.0	41.0
33	34.70555657891094	36.0	34.0	40.0	25.0	41.0
34	34.32836584593566	36.0	34.0	40.0	23.0	41.0
35	34.05027805167583	36.0	34.0	40.0	21.0	41.0
36	33.67618748352097	36.0	33.0	40.0	18.0	41.0
37	33.58201109798809	36.0	33.0	40.0	18.0	41.0
38	33.440674884812324	36.0	33.0	40.0	18.0	41.0
39	33.19207658069278	36.0	33.0	40.0	18.0	41.0
40	33.041354392783056	35.0	33.0	40.0	17.0	40.0
41	32.71250455995117	35.0	32.0	39.0	15.0	40.0
42	32.920355260829204	35.0	33.0	40.0	15.0	40.0
43	32.710617974213854	35.0	32.0	39.0	15.0	40.0
44	32.463492097890565	35.0	32.0	39.0	14.0	40.0
45	32.42676206746681	35.0	32.0	39.0	15.0	40.0
46	32.27030788549496	35.0	31.0	39.0	15.0	40.0
47	32.265846056048574	35.0	32.0	39.0	15.0	40.0
48	32.28527656480067	35.0	32.0	39.0	15.0	40.0
49	32.33911107740058	35.0	32.0	39.0	15.0	40.0
50	32.43867753590472	35.0	32.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	3.0
12	4.0
13	13.0
14	42.0
15	90.0
16	216.0
17	485.0
18	902.0
19	1584.0
20	2540.0
21	3636.0
22	5170.0
23	7448.0
24	10474.0
25	15499.0
26	22209.0
27	25635.0
28	24531.0
29	24356.0
30	26159.0
31	32008.0
32	43335.0
33	66244.0
34	103874.0
35	76730.0
36	80582.0
37	111526.0
38	120562.0
39	24740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.697534676697956	22.811434130226186	27.286868183399733	22.204163009676126
2	36.634798176260325	20.620731253634418	29.750505958938167	12.993964611167094
3	28.06546103363709	18.954227119880667	39.72557220614952	13.254739640332721
4	24.590507355517268	25.158048208465917	36.075805350583494	14.17563908543332
5	20.368504251740607	29.522357907105818	36.78685674589845	13.322281095255123
6	18.7752001261737	36.670796206602205	34.10891631481301	10.445087352411084
7	76.3183526145526	5.233680190608968	15.720303731876076	2.727663462962361
8	77.85663633922907	3.7357287072854475	15.422928698617024	2.9847062548684624
9	74.32533791796543	5.2246505843359206	16.778212402826387	3.671799094872267
10	44.78347606130983	23.496359864724457	21.301924750873162	10.418239323092555
11	38.7053470920454	19.61001732480457	29.271816431716314	12.412819151433721
12	34.69018216929669	20.565710852743976	31.0427028139865	13.701404163972834
13	20.503225977334484	33.54980309438587	32.014890422718004	13.932080505561636
14	15.418112908604733	34.53354859914688	35.53101910544292	14.517319386805458
15	14.70946940829592	21.8906550798759	48.32091461483913	15.078960896989047
16	17.061019671298254	18.660584323881142	46.78672431167311	17.49167169314749
17	17.692851320911	18.211993484236114	35.349223032478896	28.745932162373993
18	20.992510242583382	21.055597091744414	38.94384909240418	19.008043573268033
19	25.809263412878146	22.451935405808566	33.000321453983325	18.73847972732997
20	26.687422721619647	22.365251185587304	32.58207009141573	18.36525600137732
21	21.397879366868086	25.028503457135255	35.11686116438579	18.45675601161087
22	21.399685288122697	22.065227467821494	33.11216817701881	23.422919067037
23	18.85863368813666	26.247500303996745	32.66249378462101	22.231372223245575
24	18.643488269337503	21.98889719612666	42.00596917172024	17.3616453628156
25	17.766051329097845	23.167200617384278	39.5027215233307	19.564026530187178
26	17.184424290363243	28.78710716697909	34.3295998921263	19.698868650531363
27	17.08028283134742	29.089538779751045	35.43867633195722	18.391502056944308
28	15.988543235560757	25.61314036462754	40.99272695313393	17.405589446677766
29	16.728730160450084	22.39522947841382	40.77336771807402	20.10267264306207
30	19.186709382724075	26.094117392105236	36.79046858840767	17.92870463676302
31	26.329729918456636	23.98720926172735	31.66682919957958	18.016231620236432
32	26.964571436827068	23.566670398903927	32.06846608660476	17.400292077664243
33	25.286870591294736	24.801198168555057	30.26278562149576	19.649145618654444
34	19.823958796100655	25.311671909858042	32.75339182110304	22.11097747293827
35	19.304335053774317	25.281693617031525	33.86740444569655	21.54656688349761
36	27.565582030361146	24.552342219669853	29.183205895490133	18.698869854478865
37	20.263038450471406	29.61373752258907	30.92002056342335	19.20320346351618
38	21.523691880939225	29.800590175066006	27.87150509089202	20.80421285310275
39	21.21981553116358	28.542344639604334	29.214026951568805	21.023812877663282
40	23.80613555726516	25.29505743431563	28.81576111755223	22.083045890866977
41	19.277848208706708	23.760867131149613	30.146965871700132	26.814318788443547
42	22.475773566369412	24.050657295139303	28.074249850409522	25.399319288081763
43	21.734262299226703	25.04945214368873	28.84092362036646	24.37536193671811
44	20.511412820355382	28.050050505597753	29.58676909852023	21.851767575526637
45	18.458802722366094	32.6870543136837	26.865968136325385	21.988174827624814
46	21.62939847170904	29.397267761536526	28.208610391752476	20.764723375001957
47	21.01333853438655	26.311189126909312	29.982025063779115	22.693447274925024
48	22.05162286103677	24.498044187281256	32.05895490133048	21.39137805035149
49	21.343099755478264	23.94338557261549	32.71077207949424	22.002742592412
50	19.58930942775171	29.61674739134675	29.833939520901133	20.96000366000041
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	284.0
1	590.5
2	897.0
3	11166.0
4	21435.0
5	15231.0
6	9027.0
7	9205.0
8	9383.0
9	9768.0
10	10153.0
11	10104.5
12	10056.0
13	9492.5
14	8929.0
15	8437.0
16	7945.0
17	7568.5
18	7192.0
19	6376.5
20	5561.0
21	5514.0
22	5467.0
23	5295.0
24	5123.0
25	5679.0
26	6235.0
27	7210.5
28	8186.0
29	8716.5
30	9247.0
31	11095.5
32	12944.0
33	14170.0
34	15396.0
35	18519.0
36	21642.0
37	23861.5
38	26081.0
39	32053.5
40	38026.0
41	48346.0
42	58666.0
43	67030.5
44	75395.0
45	75402.0
46	75409.0
47	69697.0
48	63985.0
49	63287.5
50	62590.0
51	58227.5
52	53865.0
53	49343.0
54	44821.0
55	40834.5
56	36848.0
57	32968.0
58	29088.0
59	26593.0
60	24098.0
61	21697.5
62	19297.0
63	17418.5
64	15540.0
65	12823.0
66	10106.0
67	8905.0
68	7704.0
69	6565.5
70	5427.0
71	4473.5
72	3520.0
73	2911.0
74	2302.0
75	1741.0
76	1180.0
77	963.0
78	746.0
79	572.5
80	399.0
81	296.5
82	194.0
83	141.5
84	89.0
85	75.0
86	61.0
87	47.0
88	33.0
89	23.0
90	13.0
91	10.5
92	8.0
93	7.0
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	830601.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.6308553740462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33677570058448	22.026622497569306
2	7.529324907188243	4.462006747781973
3	3.5737561244643876	3.1768035259834835
4	2.2988543721591586	2.724680857097672
5	1.6916833832534488	2.5063012833930056
6	1.3156807872927267	2.3390848276009217
7	1.0245459479237697	2.1250720964896
8	0.8195265786514534	1.9426618821766488
9	0.6945995563263028	1.8523421096743202
>10	5.874752396458631	36.85448558166099
>50	0.7680009557185612	15.376493071765571
>100	0.071680088962103	2.8084586263596676
>500	0.0	0.0
>1k	4.096005083548743E-4	0.12658696401439642
>5k	0.0	0.0
>10k+	4.096005083548743E-4	1.6783999284324909
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13829	1.664939001999757	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1043	0.12557172457052182	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.203947503073076E-4	0.0	0.0	0.028894740073753825	0.0
2	2.407895006146152E-4	0.0	0.0	0.10390066951520646	0.0
3	3.611842509219228E-4	0.0	0.0	0.14242698961354489	0.0
4	4.815790012292304E-4	0.0	0.0	0.22140594581513867	0.0
5	4.815790012292304E-4	0.0	0.0	0.37189938369927317	0.0
6	4.815790012292304E-4	0.0	0.0	0.5793395384787642	0.0
7	4.815790012292304E-4	0.0	0.0	0.7340467926236545	0.0
8	4.815790012292304E-4	0.0	0.0	1.1791461845097706	0.0
9	4.815790012292304E-4	0.0	0.0	1.4068126573408892	0.0
10	4.815790012292304E-4	0.0	0.0	1.7512620379700963	0.0
11	4.815790012292304E-4	0.0	0.0	1.9630364037606505	0.0
12	4.815790012292304E-4	0.0	0.0	2.153862082997733	0.0
13	6.01973751536538E-4	0.0	0.0	2.2298311704416443	0.0
14	6.01973751536538E-4	0.0	0.0	2.260652226520315	0.0
15	6.01973751536538E-4	0.0	0.0	2.295446309359127	0.0
16	8.427632521511532E-4	0.0	0.0	2.3763515815656375	0.0
17	9.631580024584608E-4	0.0	0.0	2.4786871193268487	0.0
18	9.631580024584608E-4	0.0	0.0	2.636765426480344	0.0
19	0.0010835527527657683	0.0	0.0	2.7051496446548944	0.0
20	0.001203947503073076	0.0	0.0	2.7889443908687808	0.0
21	0.001203947503073076	0.0	0.0	2.8799628221011053	0.0
22	0.001203947503073076	0.0	0.0	2.9802516491070925	0.0
23	0.001203947503073076	0.0	0.0	3.081503634115538	0.0
24	0.001203947503073076	0.0	0.0	3.1654187750797314	0.0
25	0.001203947503073076	0.0	0.0	3.2351273355076624	0.0
26	0.001203947503073076	0.0	0.0	3.2995385269220723	0.0
27	0.001203947503073076	0.0	0.0	3.374905640614447	0.0
28	0.001203947503073076	0.0	0.0	3.4500319648062066	0.0
29	0.0013243422533803836	0.0	0.0	3.5359938165256244	0.0
30	0.0013243422533803836	0.0	0.0	3.662649093848912	0.0
31	0.0013243422533803836	0.0	0.0	3.7588444993444505	0.0
32	0.0013243422533803836	0.0	0.0	3.8473346408203217	0.0
33	0.0013243422533803836	0.0	0.0	3.935463598045271	0.0
34	0.0013243422533803836	0.0	0.0	4.02672281877821	0.0
35	0.0013243422533803836	0.0	0.0	4.140375463068309	0.0
36	0.0013243422533803836	0.0	0.0	4.23645047381354	0.0
37	0.0015651317539949988	0.0	0.0	4.3319235108072345	0.0
38	0.0016855265043023064	0.0	0.0	4.446659707850099	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTC	20	7.855218E-4	44.000004	34
ACTATCG	20	7.855218E-4	44.000004	1
GTATAAC	20	7.855218E-4	44.000004	28
TTACCGA	20	7.855218E-4	44.000004	37
TAGTACG	20	7.855218E-4	44.000004	1
TTGTACG	75	0.0	44.0	1
TACGGGT	50	2.7284841E-11	44.0	4
TCGATAG	45	4.802132E-10	44.0	1
TACTACG	35	1.4454417E-7	44.0	1
CGGTCTA	70	0.0	44.0	31
CGTTTTT	9365	0.0	42.942875	1
TACGGGA	185	0.0	42.810814	4
ACTACGG	60	3.6379788E-12	40.333332	2
GTTTTTT	10525	0.0	38.983376	2
TAAGGGA	595	0.0	38.82353	4
CGACGGT	80	0.0	38.500004	28
GACGGTC	80	0.0	38.500004	29
CGATGAT	40	4.122703E-7	38.500004	10
GGTACCT	195	0.0	38.358974	8
GGGCCTA	150	0.0	38.13333	7
>>END_MODULE