FastQCFastQC Report
Thu 26 May 2016
SRR1545990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545990_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences704763
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69880.9915389996353383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG454.783942E-1044.01
TACGGCA408.305506E-944.04
ATGTACG207.854406E-444.01
TATTGCG302.526318E-644.01
TGTTGCG254.4410826E-544.01
ACGATAT207.854406E-444.021
CGTAGCC207.854406E-444.028
GTCGTAG302.526318E-644.01
CATCGAA207.854406E-444.036
CGCACGG502.7284841E-1144.02
TAGTGCG207.854406E-444.01
CGATCTT207.854406E-444.010
TTCGAAT207.854406E-444.016
TCGAACG207.854406E-444.01
ACGACGT302.526318E-644.026
AATCGAG351.4450234E-744.01
TAGTACG207.854406E-444.01
CGTTTTT82650.043.2546921
CGGAATT2800.041.6428579
TCGCAAT800.041.2521
Produced by FastQC (version 0.11.5)