##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545990_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 704763 Sequences flagged as poor quality 0 Sequence length 50 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.707060387676425 28.0 25.0 30.0 16.0 33.0 2 25.89517327101451 30.0 25.0 31.0 10.0 33.0 3 26.218985673197942 30.0 25.0 31.0 16.0 33.0 4 28.023202126104803 32.0 19.0 35.0 10.0 35.0 5 31.09482762290302 33.0 28.0 35.0 19.0 37.0 6 29.604790546609287 33.0 28.0 35.0 17.0 36.0 7 29.596797788760192 32.0 28.0 35.0 17.0 35.0 8 32.32517172439529 33.0 32.0 35.0 28.0 35.0 9 26.904115284145167 30.0 23.0 34.0 10.0 35.0 10 31.49857327924423 34.0 30.0 37.0 17.0 37.0 11 33.57307492022141 35.0 32.0 37.0 27.0 39.0 12 32.94659481272428 35.0 31.0 37.0 23.0 39.0 13 33.020406860178525 35.0 31.0 37.0 22.0 39.0 14 32.72498840035586 35.0 30.0 38.0 21.0 40.0 15 31.71216848784627 34.0 27.0 38.0 18.0 40.0 16 30.303083447910858 33.0 26.0 37.0 17.0 40.0 17 30.49899753534167 32.0 26.0 37.0 17.0 40.0 18 31.412795507142118 34.0 27.0 38.0 18.0 39.0 19 31.11356725594278 34.0 27.0 37.0 18.0 39.0 20 31.886969094575054 34.0 30.0 37.0 21.0 39.0 21 32.647968182211606 34.0 31.0 37.0 23.0 39.0 22 33.03889676387665 34.0 31.0 37.0 25.0 39.0 23 34.34627669159703 35.0 33.0 38.0 28.0 40.0 24 33.95599371703679 35.0 33.0 38.0 27.0 40.0 25 32.687101905179475 35.0 31.0 38.0 23.0 39.0 26 26.43311155665096 33.0 20.0 36.0 0.0 39.0 27 29.681406373490095 34.0 24.0 37.0 15.0 39.0 28 31.186631534288832 34.0 27.0 37.0 19.0 39.0 29 32.52944039343723 34.0 30.0 37.0 23.0 39.0 30 32.27694274529168 34.0 30.0 37.0 22.0 39.0 31 31.1952159804076 34.0 29.0 36.0 19.0 39.0 32 31.475264734385885 34.0 30.0 37.0 19.0 39.0 33 31.247700290736034 34.0 30.0 37.0 18.0 39.0 34 31.25156825769798 34.0 30.0 37.0 16.0 39.0 35 30.468058056396263 34.0 29.0 37.0 12.0 39.0 36 30.203059752001735 34.0 27.0 37.0 10.0 39.0 37 30.24380962110667 34.0 27.0 37.0 10.0 39.0 38 29.999530338567716 34.0 27.0 37.0 10.0 39.0 39 29.876977083076156 34.0 27.0 37.0 10.0 39.0 40 29.776744806410097 33.0 27.0 36.0 10.0 38.0 41 29.1818625552136 33.0 25.0 36.0 10.0 38.0 42 29.360934101251058 33.0 26.0 36.0 10.0 38.0 43 28.900721235365648 33.0 25.0 36.0 10.0 38.0 44 28.653623984232997 32.0 24.0 35.0 9.0 38.0 45 28.147341730482445 32.0 23.0 35.0 9.0 38.0 46 27.743693979394493 31.0 23.0 35.0 8.0 38.0 47 27.834550054415455 31.0 23.0 35.0 8.0 38.0 48 27.897505970092073 31.0 23.0 35.0 8.0 37.0 49 27.731990754338693 31.0 24.0 35.0 8.0 37.0 50 29.61776512104069 34.0 26.0 37.0 10.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 19.0 12 30.0 13 115.0 14 279.0 15 566.0 16 1083.0 17 2051.0 18 3297.0 19 5108.0 20 7638.0 21 10505.0 22 13992.0 23 18375.0 24 23581.0 25 29562.0 26 34565.0 27 38629.0 28 42954.0 29 50126.0 30 60065.0 31 70316.0 32 75971.0 33 74863.0 34 65005.0 35 47908.0 36 23485.0 37 4567.0 38 103.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.94574346269597 23.671787537086935 26.965093229922683 23.41737577029441 2 36.41238260237839 17.687364404771532 31.71775476294868 14.1824982299014 3 29.435994795413496 14.498774765417593 41.650029868196825 14.415200570972086 4 25.907716494764905 24.868501893544355 35.16430346087976 14.05947815081098 5 19.611841143760383 29.7650415813543 37.6946576366807 12.928459638204618 6 18.811714008822825 35.237945238328344 35.29370866518248 10.656632087666349 7 79.52304533580794 3.4163257719261653 15.41908414601788 1.6415447462480293 8 81.71002166685822 1.9620780319057611 14.76155814082181 1.56634216041421 9 69.22610863510144 3.0095223500666184 15.013273965858026 12.751095048973909 10 46.252002446212416 23.322592133809522 21.124690144062612 9.30071527591545 11 40.01288376376172 18.95800432202031 29.533758156997457 11.495353757220512 12 35.54897178200331 20.40771152855641 31.363593151172807 12.679723538267474 13 19.752739573445258 34.61333810089349 32.40238207737921 13.231540248282048 14 14.588592193404024 35.00651992230012 36.27545713949228 14.129430744803573 15 13.134912020069159 20.092144451397136 49.14361281735846 17.629330711175246 16 15.311955934122533 16.398278570242763 47.30611567292834 20.983649822706358 17 16.67397408774297 16.96925065589425 36.243815296773526 30.112959959589254 18 20.41835340391025 20.413812870425946 40.47161953734801 18.69621418831579 19 26.50124935616654 21.501838206602784 33.76425833932826 18.232654097902415 20 27.41063307807022 21.678067662462418 33.258698314185054 17.652600945282316 21 21.051048366614026 24.79344119938192 35.97237652941485 18.1831339045892 22 21.067224017151865 21.566540808754148 33.35277249231302 24.013462681780968 23 17.992431498248347 26.24357408093217 33.402860252311775 22.36113416850771 24 17.70098600522445 21.105392876754316 44.618261741890535 16.5753593761307 25 17.004156007054856 21.807330975093755 41.71870543714696 19.469807580704433 26 13.495600648728722 40.70815295354608 28.730083730275286 17.06616266744991 27 15.916272562549397 30.139493702138164 36.07652501621112 17.867708719101316 28 15.184395321547811 25.387115952454938 42.77480514726227 16.653683578734977 29 15.935853613200464 21.94922264647832 42.313940998605204 19.80098274171601 30 18.630092669450583 26.108209426431294 37.674792802686866 17.58690510143126 31 27.079032242044487 23.69449020450847 32.25027420565495 16.976203347792097 32 27.439720870704054 22.685072854278673 32.87374620971873 17.001460065298545 33 25.7744802153348 24.259360948290418 30.746506272321334 19.219652564053447 34 19.28676732461835 24.678934620574577 33.11652285945772 22.917775195349357 35 19.027531240998748 24.49263085604664 34.524230131263984 21.955607771690623 36 27.834321608824524 23.921942553737924 29.173069528337898 19.070666309099654 37 19.599212785007158 30.130554526840935 30.795458898949008 19.474773789202896 38 20.70312998837907 30.500608005811884 27.827510808598067 20.968751197210977 39 20.704407013421534 28.800887674296185 29.05657646613117 21.438128846151116 40 23.78076034071028 24.558042916554925 28.862042984662928 22.799153758071864 41 18.46876183908633 23.08662628429699 30.31515559131226 28.12945628530442 42 21.77228373226177 23.197443679648337 27.464410021524966 27.56586256656493 43 21.174352229047212 24.2089894049489 28.90957669457676 25.707081671427133 44 19.693003179792356 27.441423570760666 29.97858854678807 22.886984702658907 45 17.640256369871857 33.74964349717565 26.34275635922998 22.267343773722516 46 20.764427190417205 29.31850849150707 27.980895705364784 21.93616861271094 47 20.28227929105245 25.84656118439816 29.574622958356212 24.296536566193176 48 21.251825081623185 23.8863277442204 32.98385414671315 21.877993027443267 49 20.70242053002215 23.640713261053715 32.77995581493353 22.876910393990606 50 19.020862332443674 30.365952809667927 29.694237637333405 20.918947220554994 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 154.0 1 327.0 2 500.0 3 6317.0 4 12134.0 5 9808.5 6 7483.0 7 7469.0 8 7455.0 9 7988.0 10 8521.0 11 9060.5 12 9600.0 13 9460.0 14 9320.0 15 9005.0 16 8690.0 17 8331.0 18 7972.0 19 7415.0 20 6858.0 21 6622.0 22 6386.0 23 6065.5 24 5745.0 25 5976.0 26 6207.0 27 6945.5 28 7684.0 29 8183.5 30 8683.0 31 10065.5 32 11448.0 33 12551.5 34 13655.0 35 15609.0 36 17563.0 37 19533.5 38 21504.0 39 26150.5 40 30797.0 41 39061.0 42 47325.0 43 54716.5 44 62108.0 45 62125.0 46 62142.0 47 58339.0 48 54536.0 49 52741.0 50 50946.0 51 47571.0 52 44196.0 53 41067.5 54 37939.0 55 34733.5 56 31528.0 57 28697.0 58 25866.0 59 23382.5 60 20899.0 61 18871.0 62 16843.0 63 15177.5 64 13512.0 65 11290.5 66 9069.0 67 7970.0 68 6871.0 69 5837.0 70 4803.0 71 3951.5 72 3100.0 73 2596.0 74 2092.0 75 1616.0 76 1140.0 77 922.5 78 705.0 79 548.5 80 392.0 81 284.0 82 176.0 83 148.0 84 120.0 85 85.0 86 50.0 87 37.0 88 24.0 89 16.0 90 8.0 91 7.0 92 6.0 93 4.0 94 2.0 95 3.5 96 5.0 97 3.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 704763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.32253690247667 #Duplication Level Percentage of deduplicated Percentage of total 1 81.43257041077374 37.72163247916596 2 7.151109245409662 6.625150438282621 3 2.923018791595968 4.062049375210109 4 1.6798684606526757 3.11263075039561 5 1.1124434310880256 2.5765600944246407 6 0.8235820806551457 2.28902467940199 7 0.6013377800659246 1.9498844055570033 8 0.4694792558521777 1.739797612332783 9 0.38475278244321925 1.604045246675094 >10 3.1858482709886093 29.719415840677687 >50 0.219899298710416 6.483668848248582 >100 0.01575497943602681 1.0310515941245653 >500 0.0 0.0 >1k 0.0 0.0 >5k 3.3521232842609886E-4 1.0850886355033746 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6988 0.9915389996353383 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.020716184022146453 0.0 2 0.0 0.0 0.0 0.08073636101781734 0.0 3 0.0 0.0 0.0 0.10840523693780746 0.0 4 0.0 0.0 0.0 0.15906056362209708 0.0 5 0.0 0.0 0.0 0.2707293090017495 0.0 6 0.0 0.0 0.0 0.4036818050890867 0.0 7 0.0 0.0 0.0 0.49066139964782485 0.0 8 0.0 0.0 0.0 0.7718906923320322 0.0 9 0.0 0.0 0.0 0.957768781845812 0.0 10 0.0 0.0 0.0 1.1940184147011124 0.0 11 0.0 0.0 0.0 1.347970878153365 0.0 12 0.0 0.0 0.0 1.4820585076117787 0.0 13 0.0 0.0 0.0 1.5393827428511428 0.0 14 0.0 0.0 0.0 1.5574029851169826 0.0 15 0.0 0.0 0.0 1.5798218691957437 0.0 16 0.0 0.0 0.0 1.6299096291944952 0.0 17 0.0 0.0 0.0 1.7096527485126205 0.0 18 0.0 0.0 0.0 1.8142269103230448 0.0 19 0.0 0.0 0.0 1.8590646784805673 0.0 20 0.0 0.0 0.0 1.9074497384227038 0.0 21 0.0 0.0 0.0 1.9669023487328365 0.0 22 0.0 0.0 0.0 2.0226657755869706 0.0 23 0.0 0.0 0.0 2.0852400026675633 0.0 24 0.0 0.0 0.0 2.1344764126380076 0.0 25 0.0 0.0 0.0 2.1725033805690708 0.0 26 0.0 0.0 0.0 2.212800615242287 0.0 27 0.0 0.0 0.0 2.263030266912423 0.0 28 0.0 0.0 0.0 2.320638285494556 0.0 29 0.0 0.0 0.0 2.3860503460028406 0.0 30 0.0 0.0 0.0 2.477854257388654 0.0 31 0.0 0.0 0.0 2.5472392846957064 0.0 32 0.0 0.0 0.0 2.6187526870735267 0.0 33 0.0 0.0 0.0 2.6821782641824274 0.0 34 0.0 0.0 0.0 2.7551105832740936 0.0 35 0.0 0.0 0.0 2.8473401696740606 0.0 36 0.0 0.0 0.0 2.9148806052531135 0.0 37 0.0 0.0 0.0 2.9878129243447797 0.0 38 0.0 0.0 0.0 3.0862857442856675 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTACG 45 4.783942E-10 44.0 1 TACGGCA 40 8.305506E-9 44.0 4 ATGTACG 20 7.854406E-4 44.0 1 TATTGCG 30 2.526318E-6 44.0 1 TGTTGCG 25 4.4410826E-5 44.0 1 ACGATAT 20 7.854406E-4 44.0 21 CGTAGCC 20 7.854406E-4 44.0 28 GTCGTAG 30 2.526318E-6 44.0 1 CATCGAA 20 7.854406E-4 44.0 36 CGCACGG 50 2.7284841E-11 44.0 2 TAGTGCG 20 7.854406E-4 44.0 1 CGATCTT 20 7.854406E-4 44.0 10 TTCGAAT 20 7.854406E-4 44.0 16 TCGAACG 20 7.854406E-4 44.0 1 ACGACGT 30 2.526318E-6 44.0 26 AATCGAG 35 1.4450234E-7 44.0 1 TAGTACG 20 7.854406E-4 44.0 1 CGTTTTT 8265 0.0 43.254692 1 CGGAATT 280 0.0 41.642857 9 TCGCAAT 80 0.0 41.25 21 >>END_MODULE