FastQCFastQC Report
Thu 26 May 2016
SRR1545989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545989_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences707884
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116241.64207695046081No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8970.1267156765797786No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA254.4411012E-544.013
TCGTTGA750.044.024
ACGTTAG207.8544306E-444.01
TTATACG207.8544306E-444.01
TACGCGG408.305506E-944.02
ACGCTAG351.4450234E-744.01
GCGATAC408.305506E-944.09
CGTCGCA207.8544306E-444.02
ATACGCG302.5263325E-644.012
CCTATAG254.4411012E-544.01
CGATACG302.5263325E-644.010
TCGCGCG207.8544306E-444.011
AATCGTT750.044.022
TACGTAG302.5263325E-644.01
TACCGAC207.8544306E-444.025
ACGCGTA302.5263325E-644.014
CGTTTTT98000.043.371431
TGCGTAG850.041.4117661
CGGGATA800.041.256
TATAGCG650.040.6153871
Produced by FastQC (version 0.11.5)