##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545989_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	707884
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.287620570601963	31.0	28.0	33.0	25.0	33.0
2	28.839932813850858	31.0	28.0	33.0	16.0	33.0
3	28.326895367037537	31.0	27.0	33.0	16.0	34.0
4	32.13914002859226	35.0	32.0	35.0	25.0	37.0
5	33.69658305598092	35.0	33.0	35.0	30.0	37.0
6	33.528340801600265	35.0	33.0	37.0	28.0	37.0
7	34.35406507280854	35.0	35.0	37.0	31.0	37.0
8	34.767340411705874	35.0	35.0	37.0	32.0	37.0
9	36.631699543993086	39.0	37.0	39.0	32.0	39.0
10	36.4170485559781	39.0	35.0	39.0	32.0	39.0
11	36.40047239378203	39.0	35.0	39.0	32.0	39.0
12	35.174860570375934	37.0	34.0	39.0	30.0	39.0
13	35.085894016533786	37.0	34.0	39.0	27.0	39.0
14	35.89937193099434	38.0	34.0	40.0	27.0	41.0
15	36.556256392290265	38.0	35.0	40.0	31.0	41.0
16	36.223703318622825	38.0	34.0	40.0	31.0	41.0
17	35.78750331975295	37.0	34.0	40.0	30.0	41.0
18	35.410825785015625	37.0	34.0	39.0	29.0	40.0
19	34.81487362336202	37.0	33.0	39.0	27.0	40.0
20	35.22335439139746	35.0	34.0	39.0	30.0	40.0
21	35.40276796763312	35.0	34.0	39.0	30.0	40.0
22	35.7242952235112	36.0	34.0	39.0	31.0	41.0
23	36.06255968492013	36.0	35.0	40.0	32.0	41.0
24	35.74184612168095	35.0	35.0	40.0	31.0	41.0
25	35.35860112673828	35.0	34.0	39.0	30.0	41.0
26	35.42813229286154	35.0	34.0	40.0	30.0	41.0
27	35.724223177808796	35.0	34.0	40.0	31.0	41.0
28	35.67855326578931	36.0	35.0	40.0	31.0	41.0
29	35.84614852150917	36.0	35.0	40.0	31.0	41.0
30	35.48498059004017	36.0	35.0	40.0	30.0	41.0
31	34.79390267331936	35.0	34.0	39.0	29.0	41.0
32	34.6375423091919	35.0	34.0	40.0	27.0	41.0
33	34.21527679676331	35.0	34.0	40.0	23.0	41.0
34	33.78031570144261	35.0	34.0	40.0	21.0	41.0
35	33.44070638692215	35.0	33.0	40.0	18.0	41.0
36	32.98953783388239	35.0	33.0	40.0	15.0	41.0
37	32.84729277678264	35.0	33.0	40.0	15.0	41.0
38	32.71468347921411	35.0	33.0	40.0	15.0	41.0
39	32.5476928423301	35.0	32.0	40.0	12.0	40.0
40	32.388747308881115	35.0	32.0	39.0	12.0	40.0
41	32.1177918981076	35.0	31.0	39.0	10.0	40.0
42	32.25754360883987	35.0	32.0	39.0	10.0	40.0
43	32.00132507585989	35.0	31.0	39.0	10.0	40.0
44	31.818912420679094	35.0	31.0	39.0	10.0	40.0
45	31.745163049313163	35.0	31.0	39.0	10.0	40.0
46	31.577317752626136	35.0	31.0	38.0	10.0	40.0
47	31.573439998643845	35.0	31.0	38.0	10.0	40.0
48	31.617917624921596	35.0	31.0	38.0	10.0	40.0
49	31.604404111408083	35.0	31.0	38.0	10.0	40.0
50	31.722975798294637	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	5.0
13	8.0
14	29.0
15	78.0
16	204.0
17	472.0
18	885.0
19	1457.0
20	2233.0
21	3296.0
22	4781.0
23	6682.0
24	10148.0
25	15670.0
26	22245.0
27	25254.0
28	23083.0
29	21218.0
30	22558.0
31	27008.0
32	36066.0
33	57214.0
34	93071.0
35	63231.0
36	62287.0
37	87800.0
38	98956.0
39	21943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.92682416893163	22.13498256776534	28.253781693045752	24.68441157025727
2	38.34455927807381	20.97222143741065	28.38911460069729	12.294104683818253
3	26.369433409993725	18.422227370586143	42.48407931242972	12.724259906990412
4	23.35396759921117	24.562216408338088	38.76327194851134	13.320544043939403
5	19.909900492171033	29.00800131094925	38.59347011657277	12.488628080306944
6	17.815065745235096	36.19081657446701	35.76009628696227	10.234021393335631
7	74.59258861621394	4.762079662769606	18.22728017584802	2.4180515451684177
8	75.97544230410632	3.681676658887614	17.70586706296512	2.637013974040945
9	72.57940566533499	5.09221849907612	18.942510354803893	3.3858654807849873
10	44.25343700380288	23.2830520254731	23.105621825044782	9.357889145679236
11	39.9739222810517	17.42192223584655	31.443711116510613	11.160444366591136
12	35.9395042125546	19.209079453695804	32.65337258646897	12.198043747280627
13	18.9720349661809	35.38432850580038	33.329472060394075	12.31416446762464
14	13.592339987907623	36.72621502958112	35.88271524713089	13.798729735380372
15	12.542026659735212	20.63106384661894	53.19656892937261	13.630340564273242
16	14.241316373869164	16.920145108520604	51.43003655966232	17.408501957947912
17	14.704527860496919	17.014793384226795	37.35668555865085	30.92399319662544
18	18.953529109289093	20.48033293590475	42.06169372383046	18.504444230975697
19	24.629882862163857	21.64097507501229	34.80075831633431	18.928383746489537
20	27.08113193687101	21.080572523181765	33.869814828418214	17.968480711529008
21	19.45883788869363	25.39370857372112	36.242943759146975	18.904509778438275
22	19.73656700815388	22.004452706940686	33.90188222929181	24.357098055613633
23	16.99755892208328	26.299365432754517	34.227783083104015	22.47529256205819
24	16.89231569014132	20.989173367387878	45.66355504574196	16.454955896728844
25	14.96572884822937	23.011397347588023	42.63876567347192	19.384108130710683
26	14.936486769018654	30.390854998841615	36.40356329568121	18.269094936458515
27	15.987647693689928	29.604426713981386	37.29509354640026	17.112832045928428
28	13.825711557260794	24.626916274417844	45.511694006362625	16.03567816195874
29	14.61030338304016	21.604245893394964	44.19325765238372	19.592193071181153
30	17.502867701487816	26.811454984149947	38.6927801730227	16.99289714133954
31	25.97431217544117	23.633109379502855	33.041430516864345	17.351147928191622
32	26.778822518943784	23.10124257646733	33.93635115357883	16.18358375101005
33	25.27349113696594	24.39114318165123	31.938566205762527	18.3967994756203
34	18.854219052839166	25.617615315503674	33.87730193082483	21.65086370083234
35	19.495143272061526	24.052952178605537	35.41328805284481	21.038616496488125
36	27.715557916268768	23.67125122195162	30.475897180894044	18.13729368088557
37	20.387379853196286	29.291381073735245	31.951703951494874	18.36953512157359
38	20.73616581247775	30.11397347588023	28.568098728040187	20.581761983601833
39	20.39656214860062	28.56541467246046	31.144933350661972	19.89308982827695
40	22.73719988020636	25.15765294878822	29.210859406343413	22.894287764662007
41	17.767600341298856	23.161986992219063	31.37378440535455	27.696628261127533
42	21.3410671804985	23.674500341863922	28.693684275954816	26.290748201682764
43	21.601279305648948	25.069926711156064	29.26835470218284	24.060439281012144
44	19.741935119313332	28.5502992015641	30.344378457487387	21.363387221635183
45	17.655858869532295	34.14782648004475	26.371552401240884	21.82476224918207
46	21.16038786015788	30.200710850930378	28.689305027377365	19.949596261534374
47	20.77416638884337	25.636121172395477	30.5778912929237	23.011821145837455
48	21.981567601471426	22.80599646269728	33.130004350995364	22.082431584835934
49	20.493188149470818	23.11904210294342	34.11900819908347	22.268761548502297
50	18.88911177537563	29.974967650066958	30.165253064061343	20.970667510496067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	309.0
1	537.5
2	766.0
3	9594.5
4	18423.0
5	13795.5
6	9168.0
7	9572.5
8	9977.0
9	10580.5
10	11184.0
11	11275.5
12	11367.0
13	10915.5
14	10464.0
15	9791.5
16	9119.0
17	8552.0
18	7985.0
19	7236.5
20	6488.0
21	6081.0
22	5674.0
23	5325.0
24	4976.0
25	4921.5
26	4867.0
27	5400.0
28	5933.0
29	7429.0
30	8925.0
31	10359.5
32	11794.0
33	13515.5
34	15237.0
35	15896.5
36	16556.0
37	17887.5
38	19219.0
39	23747.5
40	28276.0
41	40842.0
42	53408.0
43	61126.5
44	68845.0
45	67073.0
46	65301.0
47	61021.0
48	56741.0
49	55533.5
50	54326.0
51	50150.5
52	45975.0
53	40946.5
54	35918.0
55	31503.5
56	27089.0
57	23942.0
58	20795.0
59	19338.5
60	17882.0
61	16294.5
62	14707.0
63	12338.5
64	9970.0
65	8371.5
66	6773.0
67	5691.0
68	4609.0
69	4002.5
70	3396.0
71	2859.5
72	2323.0
73	1886.0
74	1449.0
75	1095.0
76	741.0
77	605.0
78	469.0
79	349.5
80	230.0
81	162.5
82	95.0
83	72.0
84	49.0
85	36.0
86	23.0
87	26.5
88	30.0
89	24.0
90	18.0
91	14.0
92	10.0
93	5.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	707884.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.234811714689975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.48656498267647	23.890434559372988
2	7.360198162619549	4.597888075844574
3	3.368342623522054	3.1562864290882864
4	2.101450169681852	2.625536015112638
5	1.4734557861772473	2.3011557025583405
6	1.0722203624429827	2.0094360682537924
7	0.8588793200817202	1.8778853693875435
8	0.6791337038790028	1.6970090695808535
9	0.5704298559053707	1.6035542231077786
>10	5.227333138561313	35.7267157977299
>50	0.7202113125424239	15.15536038454702
>100	0.08087692907119909	3.59168549347774
>500	4.5182641939217367E-4	0.1265910368428107
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.5182641939217367E-4	1.6404617750956876
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11624	1.64207695046081	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	897	0.1267156765797786	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033903859954455814	0.0
2	0.0	0.0	0.0	0.13434404506953115	0.0
3	0.0	0.0	0.0	0.17658260392945738	0.0
4	0.0	0.0	0.0	0.2722197422176515	0.0
5	0.0	0.0	0.0	0.5047437150719609	0.0
6	0.0	0.0	0.0	0.705482819218968	0.0
7	0.0	0.0	0.0	0.8546598030185737	0.0
8	0.0	0.0	0.0	1.308830260325138	0.0
9	1.412660831435659E-4	0.0	0.0	1.574693028801329	0.0
10	1.412660831435659E-4	0.0	0.0	2.0636149425612107	0.0
11	1.412660831435659E-4	0.0	0.0	2.349254962677501	0.0
12	1.412660831435659E-4	0.0	0.0	2.663571997671935	0.0
13	1.412660831435659E-4	0.0	0.0	2.7765848641867876	0.0
14	1.412660831435659E-4	0.0	0.0	2.8179758265478525	0.0
15	1.412660831435659E-4	0.0	0.0	2.87928530663216	0.0
16	1.412660831435659E-4	0.0	0.0	2.990885512315577	0.0
17	1.412660831435659E-4	0.0	0.0	3.1232518322210985	0.0
18	1.412660831435659E-4	0.0	0.0	3.3317605709410016	0.0
19	4.237982494306977E-4	0.0	0.0	3.4278215074786265	0.0
20	4.237982494306977E-4	0.0	0.0	3.533771069836301	0.0
21	4.237982494306977E-4	0.0	0.0	3.6442411468545695	0.0
22	4.237982494306977E-4	0.0	0.0	3.7500494431291003	0.0
23	4.237982494306977E-4	0.0	0.0	3.875069926711156	0.0
24	4.237982494306977E-4	0.0	0.0	3.9663278164218996	0.0
25	4.237982494306977E-4	0.0	0.0	4.040351243989128	0.0
26	4.237982494306977E-4	0.0	0.0	4.112962010724921	0.0
27	4.237982494306977E-4	0.0	0.0	4.194613806781902	0.0
28	4.237982494306977E-4	0.0	0.0	4.272592684677151	0.0
29	4.237982494306977E-4	0.0	0.0	4.368512355131632	0.0
30	4.237982494306977E-4	0.0	0.0	4.496358160376559	0.0
31	4.237982494306977E-4	0.0	0.0	4.595385684660198	0.0
32	4.237982494306977E-4	0.0	0.0	4.682123059710348	0.0
33	4.237982494306977E-4	0.0	0.0	4.7754999406682455	0.0
34	4.237982494306977E-4	0.0	0.0	4.873397336286736	0.0
35	4.237982494306977E-4	0.0	0.0	4.996863892954213	0.0
36	4.237982494306977E-4	0.0	0.0	5.104226116143323	0.0
37	4.237982494306977E-4	0.0	0.0	5.202829842177532	0.0
38	5.650643325742636E-4	1.412660831435659E-4	0.0	5.311745992281221	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	25	4.4411012E-5	44.0	13
TCGTTGA	75	0.0	44.0	24
ACGTTAG	20	7.8544306E-4	44.0	1
TTATACG	20	7.8544306E-4	44.0	1
TACGCGG	40	8.305506E-9	44.0	2
ACGCTAG	35	1.4450234E-7	44.0	1
GCGATAC	40	8.305506E-9	44.0	9
CGTCGCA	20	7.8544306E-4	44.0	2
ATACGCG	30	2.5263325E-6	44.0	12
CCTATAG	25	4.4411012E-5	44.0	1
CGATACG	30	2.5263325E-6	44.0	10
TCGCGCG	20	7.8544306E-4	44.0	11
AATCGTT	75	0.0	44.0	22
TACGTAG	30	2.5263325E-6	44.0	1
TACCGAC	20	7.8544306E-4	44.0	25
ACGCGTA	30	2.5263325E-6	44.0	14
CGTTTTT	9800	0.0	43.37143	1
TGCGTAG	85	0.0	41.411766	1
CGGGATA	80	0.0	41.25	6
TATAGCG	65	0.0	40.615387	1
>>END_MODULE