FastQCFastQC Report
Thu 26 May 2016
SRR1545988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545988_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences705564
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119091.6878695625060236No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9250.13110079312436576No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAG207.8544114E-444.00000429
CAACCCG207.8544114E-444.00000423
ACGATAT207.8544114E-444.00000422
CGCTATA207.8544114E-444.00000422
TATTACG207.8544114E-444.0000041
TCGTAAG207.8544114E-444.0000041
GCGTATG207.8544114E-444.0000041
CCCATAC207.8544114E-444.00000411
ATCGAGC207.8544114E-444.00000429
TCTAGCG454.783942E-1044.01
TTATACG254.441088E-544.01
ACTACCG254.441088E-544.01
CAAATCG254.441088E-544.039
ACGCTAG254.441088E-544.01
GCGATAC302.5263216E-644.09
CGTAAGG454.783942E-1044.02
CGGTAAT254.441088E-544.06
ATCGCCT254.441088E-544.042
CGTTTTT101750.043.437841
CGTTGAT1150.042.0869625
Produced by FastQC (version 0.11.5)