##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545988_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	705564
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.26612610620723	31.0	28.0	33.0	25.0	33.0
2	28.80820875214722	31.0	28.0	33.0	16.0	33.0
3	28.33880696860951	31.0	28.0	33.0	16.0	34.0
4	32.12993293308615	35.0	32.0	35.0	25.0	37.0
5	33.74232670601108	35.0	33.0	35.0	30.0	37.0
6	33.62356497780499	35.0	33.0	37.0	28.0	37.0
7	34.41959623790329	35.0	35.0	37.0	32.0	37.0
8	34.81977538536547	35.0	35.0	37.0	32.0	37.0
9	36.759597995362576	39.0	37.0	39.0	32.0	39.0
10	36.42631851965236	39.0	35.0	39.0	32.0	39.0
11	36.458309947786454	39.0	35.0	39.0	32.0	39.0
12	35.134191937230355	37.0	34.0	39.0	29.0	39.0
13	35.10207295156783	37.0	34.0	39.0	27.0	39.0
14	35.92761251991315	38.0	34.0	40.0	27.0	41.0
15	36.59712371946414	38.0	35.0	40.0	31.0	41.0
16	36.26986921101417	38.0	34.0	40.0	31.0	41.0
17	35.846432074198795	37.0	34.0	40.0	30.0	41.0
18	35.48783101178631	37.0	34.0	39.0	29.0	40.0
19	34.89526818261702	37.0	33.0	39.0	27.0	40.0
20	35.203091427567166	35.0	34.0	39.0	30.0	40.0
21	35.3153250449286	35.0	34.0	39.0	30.0	40.0
22	35.709367257966676	35.0	34.0	39.0	31.0	40.0
23	36.084485603006954	36.0	35.0	40.0	32.0	41.0
24	35.648778849261014	35.0	34.0	40.0	31.0	41.0
25	35.28552477167202	35.0	34.0	39.0	30.0	41.0
26	35.411364525400955	35.0	34.0	39.0	30.0	41.0
27	35.7722545367961	35.0	35.0	40.0	31.0	41.0
28	35.71883060927145	36.0	35.0	40.0	31.0	41.0
29	35.89198286760662	36.0	35.0	40.0	31.0	41.0
30	35.551514816515585	36.0	35.0	40.0	31.0	41.0
31	34.848820234592466	35.0	34.0	39.0	29.0	41.0
32	34.71911690505751	35.0	34.0	40.0	27.0	41.0
33	34.31587354230091	35.0	34.0	40.0	24.0	41.0
34	33.81638660702644	35.0	34.0	40.0	21.0	41.0
35	33.491735689462615	35.0	33.0	40.0	18.0	41.0
36	33.023518773633576	35.0	33.0	40.0	15.0	41.0
37	32.87728540571798	35.0	33.0	40.0	15.0	41.0
38	32.76843773208384	35.0	33.0	40.0	15.0	41.0
39	32.573312980821015	35.0	32.0	40.0	12.0	40.0
40	32.38158976365007	35.0	32.0	39.0	12.0	40.0
41	32.107009427918655	35.0	31.0	39.0	10.0	40.0
42	32.249899371283114	35.0	32.0	39.0	10.0	40.0
43	32.02640440838818	35.0	31.0	39.0	10.0	40.0
44	31.826217040551956	35.0	31.0	39.0	10.0	40.0
45	31.742861030324676	35.0	31.0	39.0	10.0	40.0
46	31.557284668718925	35.0	31.0	38.0	10.0	40.0
47	31.558575834367968	35.0	31.0	38.0	10.0	40.0
48	31.605480154883185	35.0	31.0	38.0	10.0	40.0
49	31.647092255273794	35.0	31.0	38.0	10.0	40.0
50	31.78903260370427	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	7.0
14	30.0
15	66.0
16	181.0
17	439.0
18	774.0
19	1306.0
20	2196.0
21	3145.0
22	4504.0
23	6540.0
24	9959.0
25	15414.0
26	22311.0
27	25031.0
28	22798.0
29	21130.0
30	22101.0
31	26301.0
32	36127.0
33	56897.0
34	94139.0
35	63654.0
36	62276.0
37	90302.0
38	98489.0
39	19442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.00864556581685	21.966823704157243	28.286590585687478	24.73794014433843
2	38.64497054838399	20.92793850026362	28.190072055830512	12.23701889552188
3	26.29300814667415	18.421007874551425	42.54156958121446	12.744414397559966
4	23.25586339439087	24.605988967691093	38.769268273324606	13.36887936459343
5	19.80316456055014	28.96831470993418	38.80838591538117	12.420134814134508
6	17.81029077447262	36.27197532753939	35.91155444438775	10.006179453600241
7	74.43846341366623	4.735360647652091	18.41973229926697	2.4064436394147095
8	75.9131701730814	3.668979709849142	17.83112517078536	2.5867249462841078
9	72.54848603386795	5.07027002511466	19.073110306081375	3.308133634936023
10	44.45549943024304	22.82882913527334	23.18173829730542	9.5339331371782
11	40.053772584769064	17.42605915267786	31.386521988083295	11.133646274469786
12	35.980152048573906	18.790924707042876	32.77519828109144	12.453724963291778
13	19.039520156924105	35.28255409856512	33.43481243374095	12.243113310769823
14	13.617616545061823	36.66896270217868	35.960026305196976	13.753394447562517
15	12.5404640826346	20.57942298643355	53.322590154826486	13.557522776105358
16	14.229325759250756	16.75028771309194	51.691979749533715	17.3284067781236
17	14.698170541580918	16.892301761427735	37.42707961290542	30.982448084085924
18	18.8986399532856	20.399425140738472	42.10475591158279	18.597178994393136
19	24.65927966846381	21.652323531245926	34.850984460658424	18.83741233963184
20	27.120147853348524	20.82036498460806	33.93979851579729	18.119688646246125
21	19.499861104024582	25.210753383109115	36.394855746608386	18.894529766257918
22	19.765180763190866	21.862509992006395	34.07415911242637	24.298150132376367
23	16.994914706532647	26.254457426966226	34.282361344966574	22.468266521534545
24	16.947860151595037	20.942395020154088	45.71137416308088	16.398370665169992
25	15.02925319319013	22.823443372961204	42.794133487536214	19.353169946312455
26	14.882278574303678	30.355290235896387	36.473516222483006	18.288914967316927
27	15.880345369094794	29.468056760265547	37.40695386952849	17.24464400111117
28	13.79775612134406	24.568572092680466	45.598698346287506	16.034973439687967
29	14.624045444495467	21.560765571939612	44.25169084590483	19.563498137660083
30	17.515207692002427	26.699491470653264	38.726040444240354	17.059260393103955
31	25.836777386601355	23.728676633161555	33.091824412810176	17.34272156742691
32	26.816986127410132	23.098825903815953	33.866948993996296	16.217238974777626
33	25.113101008554857	24.455329353538446	32.026435589117355	18.405134048789336
34	18.785680675317902	25.558135052241894	34.04581299499407	21.610371277446127
35	19.42800369633371	24.015114149814902	35.557794898832704	20.99908725501868
36	27.66269254100266	23.5890720048075	30.530610972215133	18.217624481974703
37	20.33592983769013	29.215351123356637	32.07193110759619	18.376787931357043
38	20.698334949062026	30.116757657703623	28.681593732106514	20.503313661127837
39	20.385393812609486	28.501170694649954	31.18682359077277	19.926611901967785
40	22.718846199636037	25.072424330039517	29.355664404646497	22.853065065677956
41	17.78336196291194	23.011945053885967	31.532079301098126	27.672613682103965
42	21.310752816186767	23.700047054554936	28.73899461990691	26.25020550935138
43	21.694275785045722	25.197855899677425	29.21407554807218	23.893792767204676
44	19.795936300604904	28.540004875532198	30.368896372263894	21.295162451599005
45	17.677347483715156	34.02823840218605	26.44664410315719	21.8477700109416
46	21.116865372949867	30.254803249598904	28.702711589593573	19.925619787857656
47	20.758712179192816	25.77625842588341	30.584326864749332	22.880702530174442
48	22.06362569518853	22.752152887618983	33.10089517038851	22.083326246803974
49	20.489565794173174	22.98133124705909	34.179181477513026	22.34992148125471
50	18.92571049543344	29.943846341366626	30.12313553412589	21.007307629074045
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	300.0
1	527.0
2	754.0
3	9786.0
4	18818.0
5	14071.5
6	9325.0
7	9625.0
8	9925.0
9	10598.0
10	11271.0
11	11356.5
12	11442.0
13	10977.5
14	10513.0
15	9739.5
16	8966.0
17	8364.5
18	7763.0
19	7138.5
20	6514.0
21	6082.0
22	5650.0
23	5321.5
24	4993.0
25	4962.5
26	4932.0
27	5438.5
28	5945.0
29	7263.5
30	8582.0
31	10069.5
32	11557.0
33	13299.0
34	15041.0
35	15653.5
36	16266.0
37	17742.0
38	19218.0
39	23804.5
40	28391.0
41	40470.0
42	52549.0
43	60537.5
44	68526.0
45	66865.5
46	65205.0
47	60899.0
48	56593.0
49	55218.5
50	53844.0
51	49905.0
52	45966.0
53	40769.0
54	35572.0
55	31390.5
56	27209.0
57	23897.0
58	20585.0
59	19388.5
60	18192.0
61	16474.0
62	14756.0
63	12397.5
64	10039.0
65	8458.5
66	6878.0
67	5767.0
68	4656.0
69	4014.5
70	3373.0
71	2829.5
72	2286.0
73	1867.0
74	1448.0
75	1103.0
76	758.0
77	612.0
78	466.0
79	357.0
80	248.0
81	180.5
82	113.0
83	80.0
84	47.0
85	36.5
86	26.0
87	25.0
88	24.0
89	25.5
90	27.0
91	19.0
92	11.0
93	6.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	705564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.017126065447663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.01253369328312	23.576903401186506
2	7.6183475524428665	4.725984928890301
3	3.3966730903013276	3.1606511233496994
4	2.236835369201032	2.7752081853664237
5	1.4988340142555396	2.324476178567253
6	1.0937744236226385	2.035544351079944
7	0.8705482154388975	1.8901332621023146
8	0.6711377918284823	1.6653412397144167
9	0.5320407376866317	1.485213716950203
>10	5.241847730634382	35.508594296681686
>50	0.7429866502147241	15.377300207069117
>100	0.08352289705679329	3.6478207201431365
>500	4.589170167955675E-4	0.13166715441262736
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.589170167955675E-4	1.6951612344864637
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11909	1.6878695625060236	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	925	0.13110079312436576	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02990515389107154	0.0
2	0.0	0.0	0.0	0.1284079119682977	0.0
3	0.0	0.0	0.0	0.17078535752957918	0.0
4	0.0	0.0	0.0	0.2640440838818307	0.0
5	0.0	0.0	0.0	0.48145880458753565	0.0
6	0.0	0.0	0.0	0.6820075854210249	0.0
7	0.0	0.0	0.0	0.8325254689865129	0.0
8	0.0	0.0	0.0	1.276000476214773	0.0
9	2.83461174322953E-4	0.0	0.0	1.5287061131236854	0.0
10	2.83461174322953E-4	0.0	0.0	2.0010941601328867	0.0
11	2.83461174322953E-4	0.0	0.0	2.2888072520706837	0.0
12	2.83461174322953E-4	0.0	0.0	2.6007562744130936	0.0
13	2.83461174322953E-4	0.0	0.0	2.716266702949697	0.0
14	2.83461174322953E-4	0.0	0.0	2.7586441485109785	0.0
15	2.83461174322953E-4	0.0	0.0	2.820580415100544	0.0
16	2.83461174322953E-4	0.0	0.0	2.9389254553803768	0.0
17	2.83461174322953E-4	0.0	0.0	3.074136435532425	0.0
18	2.83461174322953E-4	0.0	0.0	3.2895669280178694	0.0
19	2.83461174322953E-4	0.0	0.0	3.382258732021475	0.0
20	4.251917614844295E-4	0.0	0.0	3.481895334795993	0.0
21	4.251917614844295E-4	0.0	0.0	3.5840830881394177	0.0
22	4.251917614844295E-4	0.0	0.0	3.6956250602354994	0.0
23	4.251917614844295E-4	0.0	0.0	3.8203479769375988	0.0
24	5.66922348645906E-4	0.0	0.0	3.9124728585925586	0.0
25	5.66922348645906E-4	0.0	0.0	3.97979488749426	0.0
26	5.66922348645906E-4	0.0	0.0	4.056754596322942	0.0
27	5.66922348645906E-4	0.0	0.0	4.137966222766468	0.0
28	8.50383522968859E-4	0.0	0.0	4.2179022739255405	0.0
29	8.50383522968859E-4	0.0	0.0	4.30421620150688	0.0
30	8.50383522968859E-4	0.0	0.0	4.431206807603562	0.0
31	8.50383522968859E-4	0.0	0.0	4.530985140965242	0.0
32	8.50383522968859E-4	0.0	0.0	4.618999835592519	0.0
33	8.50383522968859E-4	0.0	0.0	4.71225856194477	0.0
34	8.50383522968859E-4	0.0	0.0	4.807926708278767	0.0
35	8.50383522968859E-4	0.0	0.0	4.930240204999121	0.0
36	9.921141101303355E-4	0.0	0.0	5.038805834764812	0.0
37	9.921141101303355E-4	0.0	0.0	5.135324364621778	0.0
38	9.921141101303355E-4	0.0	0.0	5.230709049781451	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACTAG	20	7.8544114E-4	44.000004	29
CAACCCG	20	7.8544114E-4	44.000004	23
ACGATAT	20	7.8544114E-4	44.000004	22
CGCTATA	20	7.8544114E-4	44.000004	22
TATTACG	20	7.8544114E-4	44.000004	1
TCGTAAG	20	7.8544114E-4	44.000004	1
GCGTATG	20	7.8544114E-4	44.000004	1
CCCATAC	20	7.8544114E-4	44.000004	11
ATCGAGC	20	7.8544114E-4	44.000004	29
TCTAGCG	45	4.783942E-10	44.0	1
TTATACG	25	4.441088E-5	44.0	1
ACTACCG	25	4.441088E-5	44.0	1
CAAATCG	25	4.441088E-5	44.0	39
ACGCTAG	25	4.441088E-5	44.0	1
GCGATAC	30	2.5263216E-6	44.0	9
CGTAAGG	45	4.783942E-10	44.0	2
CGGTAAT	25	4.441088E-5	44.0	6
ATCGCCT	25	4.441088E-5	44.0	42
CGTTTTT	10175	0.0	43.43784	1
CGTTGAT	115	0.0	42.08696	25
>>END_MODULE