FastQCFastQC Report
Thu 26 May 2016
SRR1545987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545987_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences692880
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120381.737385983142824No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8840.12758341992841474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG551.8189894E-1244.01
TCCGATG207.8543153E-444.018
CCGATCT207.8543153E-444.012
ACGCACC254.4410048E-544.021
TATATCG207.8543153E-444.02
TATTGCG254.4410048E-544.01
CGGGCTA207.8543153E-444.06
ACGTCGG207.8543153E-444.02
CGCACGG207.8543153E-444.02
CGTCCTA254.4410048E-544.026
GTGACGG502.7284841E-1144.02
TCGAACG254.4410048E-544.01
CGTTTTT99700.043.426281
CGCGACC1350.040.7407410
CGTTAGG1200.040.3333322
GCGATGT1050.039.8095259
CGTAAGG501.3460522E-939.62
AGGGCGC1000.039.66
GCGCGAC1400.039.2857139
CGACCCG1400.039.28571332
Produced by FastQC (version 0.11.5)