##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545987_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	692880
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.164728380094676	31.0	28.0	33.0	25.0	33.0
2	28.71511950121233	31.0	28.0	33.0	16.0	33.0
3	28.243108474771965	31.0	27.0	33.0	16.0	34.0
4	32.0111346842166	35.0	32.0	35.0	25.0	37.0
5	33.68045116037409	35.0	33.0	35.0	30.0	37.0
6	33.550503694723474	35.0	33.0	37.0	28.0	37.0
7	34.32492783743217	35.0	35.0	37.0	31.0	37.0
8	34.78599613208637	35.0	35.0	37.0	32.0	37.0
9	36.73684765038679	39.0	37.0	39.0	32.0	39.0
10	36.50330360235539	39.0	35.0	39.0	32.0	39.0
11	36.478160720471074	39.0	35.0	39.0	32.0	39.0
12	35.30757273986838	37.0	34.0	39.0	30.0	39.0
13	35.16928183812493	37.0	34.0	39.0	27.0	39.0
14	35.98650992956934	38.0	34.0	40.0	29.0	41.0
15	36.57416724396721	38.0	35.0	40.0	31.0	41.0
16	36.22144960166263	38.0	34.0	40.0	31.0	41.0
17	35.79784666897587	37.0	34.0	40.0	30.0	41.0
18	35.420716429973446	37.0	34.0	39.0	29.0	40.0
19	34.7986880845168	37.0	33.0	39.0	27.0	40.0
20	35.28322508948158	35.0	34.0	39.0	30.0	40.0
21	35.38705403533079	35.0	34.0	39.0	30.0	40.0
22	35.72358128391641	35.0	34.0	39.0	31.0	41.0
23	36.07439239117885	36.0	35.0	40.0	32.0	41.0
24	35.6667720240157	35.0	34.0	40.0	31.0	41.0
25	35.30723213254821	35.0	34.0	39.0	30.0	41.0
26	35.40601980140861	35.0	34.0	40.0	30.0	41.0
27	35.76103509987299	35.0	35.0	40.0	31.0	41.0
28	35.71396201362429	36.0	35.0	40.0	31.0	41.0
29	35.87464207366355	36.0	35.0	40.0	31.0	41.0
30	35.53658209213717	36.0	35.0	40.0	31.0	41.0
31	34.81259381133818	35.0	34.0	39.0	29.0	41.0
32	34.7011488280799	35.0	34.0	40.0	27.0	41.0
33	34.31345831890082	35.0	34.0	40.0	24.0	41.0
34	33.80411470961783	35.0	34.0	40.0	21.0	41.0
35	33.44886993418774	35.0	33.0	40.0	18.0	41.0
36	33.02153763999538	35.0	33.0	40.0	15.0	41.0
37	32.89804150790902	35.0	33.0	40.0	15.0	41.0
38	32.76628997806258	35.0	33.0	40.0	15.0	41.0
39	32.54127698880037	35.0	32.0	40.0	12.0	41.0
40	32.40930464149636	35.0	32.0	39.0	12.0	40.0
41	32.11309028980487	35.0	31.0	39.0	10.0	40.0
42	32.27939903013509	35.0	32.0	39.0	10.0	40.0
43	32.05884424431359	35.0	31.0	39.0	10.0	40.0
44	31.87078281953585	35.0	31.0	39.0	10.0	40.0
45	31.804777161990533	35.0	31.0	39.0	10.0	40.0
46	31.62510824385175	35.0	31.0	38.0	10.0	40.0
47	31.6437767001501	35.0	31.0	38.0	10.0	40.0
48	31.67061395912712	35.0	31.0	38.0	10.0	40.0
49	31.713194203902553	35.0	31.0	38.0	10.0	40.0
50	31.78821729592426	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	19.0
15	55.0
16	148.0
17	377.0
18	770.0
19	1313.0
20	2101.0
21	3183.0
22	4555.0
23	6585.0
24	9898.0
25	15089.0
26	22042.0
27	24561.0
28	22535.0
29	20680.0
30	21912.0
31	26247.0
32	35373.0
33	55840.0
34	92188.0
35	61902.0
36	60652.0
37	85814.0
38	97415.0
39	21615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.953671631451332	22.084632259554322	28.046703613901396	24.914992495092942
2	38.83053342570142	20.721770003463803	28.231295462417734	12.216401108417042
3	26.453354116152873	18.318034868952775	42.55758572913059	12.671025285763768
4	23.249769079782933	24.580591155755688	38.84770811684563	13.321931647615749
5	19.75536889504676	28.94960166262556	38.85362544740792	12.441403994919757
6	17.75314628795751	36.193424546819074	36.00046184043413	10.052967324789286
7	74.31416695531694	4.771677635376977	18.47174113843667	2.4424142708694148
8	75.80562290728552	3.6908266943770927	17.925903475349266	2.5776469229881074
9	72.45727975984298	5.124408266943771	19.110668514028404	3.3076434591848516
10	44.42544163491514	23.055507447177	23.27473732825309	9.244313589654775
11	40.097852441981296	17.336190971019512	31.468363930262093	11.097592656737097
12	35.979390370626945	19.120915598660662	32.75559981526383	12.144094215448563
13	19.03056806373398	35.22384828541739	33.477802794134625	12.267780856714005
14	13.582438517492207	36.75643690105069	35.92974252395798	13.731382057499134
15	12.580389100565755	20.50600392564369	53.40217642304583	13.511430550744718
16	14.200294423276757	16.69062463918716	51.729015125274216	17.380065812261865
17	14.635867682715622	16.92674056113613	37.45598083362198	30.981410922526265
18	18.860986029326867	20.353019281838126	42.23920447985221	18.546790208982795
19	24.655928876573142	21.573288303891005	34.94068236924143	18.830100450294424
20	27.18320632721395	20.94706154023785	33.97947696570835	17.890255166839857
21	19.4024939383443	25.239435400069276	36.4633125505138	18.894758111072623
22	19.742523957972523	21.90523611592195	34.11514259323404	24.237097332871492
23	17.00785128738021	26.199919177924023	34.33393949890313	22.458290035792633
24	16.990676596236	20.92483546934534	45.79811800023092	16.28636993418774
25	14.956702459300312	22.77695993534234	42.90800715852673	19.35833044683062
26	14.81223299849902	30.435573259438865	36.47413693568872	18.278056806373396
27	15.89683639302621	29.589250663895623	37.43274448677982	17.08116845629835
28	13.758370857868607	24.475666782126776	45.77011892391179	15.99584343609283
29	14.557787784320517	21.564628795751066	44.39109225262672	19.486491167301697
30	17.434909363814803	26.739839510449137	38.799647846668975	17.025603279067084
31	25.876630874033026	23.531347419466574	33.199832582842625	17.392189123657776
32	26.837403302159103	23.09173305622907	34.05380441057614	16.017059231035677
33	25.174633414155412	24.361649924950928	32.10310587691952	18.36061078397414
34	18.685198014086133	25.577589192933843	34.03345456644729	21.70375822653273
35	19.4541623369126	24.013249047454106	35.6335873455721	20.899001270061195
36	27.720384482161414	23.56843897933264	30.565032906130934	18.146143632375015
37	20.293413000808222	29.341733056229074	32.079003579263365	18.28585036369934
38	20.707481815032907	30.166262556286803	28.589366124004158	20.536889504676132
39	20.339164068814224	28.607551091098028	31.155900011546013	19.89738482854174
40	22.646778662971943	25.12368664126544	29.449832582842628	22.779702112919985
41	17.749971134972867	23.11006234845861	31.531434014547973	27.608532502020548
42	21.276267174691142	23.75432975406997	28.78175152984644	26.187651541392448
43	21.69293384135781	25.086306431128047	29.2967036139014	23.924056113612746
44	19.906477312088676	28.506956471539084	30.416666666666664	21.169899549705576
45	17.635954277797023	34.21010853250202	26.41727283223646	21.736664357464498
46	21.17249740214756	30.347101951275835	28.803255975060615	19.677144671515993
47	20.836941461724972	25.654225839972288	30.56373397990994	22.945098718392796
48	22.030510333679715	22.815061771158067	33.105443944117305	22.048983951044914
49	20.468190740099296	23.021446715159914	34.4117307470269	22.09863179771389
50	18.886820228611015	30.037091559866063	30.23034291652234	20.84574529500058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	553.5
2	818.0
3	9876.0
4	18934.0
5	14092.5
6	9251.0
7	9618.5
8	9986.0
9	10709.0
10	11432.0
11	11403.5
12	11375.0
13	10750.0
14	10125.0
15	9451.0
16	8777.0
17	8201.0
18	7625.0
19	7018.0
20	6411.0
21	5937.5
22	5464.0
23	5118.5
24	4773.0
25	4788.5
26	4804.0
27	5252.0
28	5700.0
29	7128.5
30	8557.0
31	9928.0
32	11299.0
33	13161.5
34	15024.0
35	15515.0
36	16006.0
37	17398.5
38	18791.0
39	23288.5
40	27786.0
41	39756.0
42	51726.0
43	59651.0
44	67576.0
45	65568.5
46	63561.0
47	59497.0
48	55433.0
49	54049.0
50	52665.0
51	48626.5
52	44588.0
53	39805.5
54	35023.0
55	30927.0
56	26831.0
57	23583.0
58	20335.0
59	18866.0
60	17397.0
61	15900.5
62	14404.0
63	12134.5
64	9865.0
65	8330.0
66	6795.0
67	5655.0
68	4515.0
69	3956.5
70	3398.0
71	2856.0
72	2314.0
73	1931.0
74	1548.0
75	1165.0
76	782.0
77	629.0
78	476.0
79	343.5
80	211.0
81	158.0
82	105.0
83	71.5
84	38.0
85	27.5
86	17.0
87	18.0
88	19.0
89	19.5
90	20.0
91	14.5
92	9.0
93	5.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	692880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.74039659619188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.87253721470864	23.323518847394713
2	7.506787934332852	4.615232765297997
3	3.514676646415237	3.2412766205453414
4	2.1917118979038768	2.6949637186463034
5	1.511446710239201	2.3231235653381277
6	1.130604100471742	2.085313106506927
7	0.8315319222129034	1.7893134749852897
8	0.6663660056391246	1.638748423325354
9	0.5485156473559528	1.5175429685045025
>10	5.410479752104122	36.211435341647515
>50	0.734750543858021	15.190921798779467
>100	0.07964903265596206	3.4964922957721902
>500	4.712960512187104E-4	0.1280723953535517
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.712960512187104E-4	1.7440446779027774
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12038	1.737385983142824	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	884	0.12758341992841474	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4432513566562752E-4	0.0	0.0	0.03261748066043182	0.0
2	1.4432513566562752E-4	0.0	0.0	0.1268617942500866	0.0
3	1.4432513566562752E-4	0.0	0.0	0.1678501327791248	0.0
4	1.4432513566562752E-4	0.0	0.0	0.25574414039949195	0.0
5	1.4432513566562752E-4	0.0	0.0	0.4806027017665397	0.0
6	1.4432513566562752E-4	0.0	0.0	0.6682253781318555	0.0
7	1.4432513566562752E-4	0.0	0.0	0.8184678443597737	0.0
8	1.4432513566562752E-4	0.0	0.0	1.255051379748297	0.0
9	1.4432513566562752E-4	0.0	0.0	1.492177577646923	0.0
10	1.4432513566562752E-4	0.0	0.0	1.9446368779586654	0.0
11	2.8865027133125504E-4	0.0	0.0	2.210916753261748	0.0
12	2.8865027133125504E-4	0.0	0.0	2.5083708578686066	0.0
13	4.329754069968826E-4	0.0	0.0	2.6281607204710773	0.0
14	5.773005426625101E-4	0.0	0.0	2.669726359542778	0.0
15	5.773005426625101E-4	0.0	0.0	2.727456413809029	0.0
16	5.773005426625101E-4	0.0	0.0	2.833968363930262	0.0
17	5.773005426625101E-4	0.0	0.0	2.966458838471308	0.0
18	5.773005426625101E-4	0.0	0.0	3.1751529846438054	0.0
19	5.773005426625101E-4	0.0	0.0	3.266366470384482	0.0
20	5.773005426625101E-4	0.0	0.0	3.3596005080244775	0.0
21	5.773005426625101E-4	0.0	0.0	3.466834083824039	0.0
22	5.773005426625101E-4	0.0	0.0	3.5792633645075624	0.0
23	7.216256783281376E-4	0.0	0.0	3.6948677981757303	0.0
24	7.216256783281376E-4	0.0	0.0	3.7784320517261287	0.0
25	8.659508139937652E-4	0.0	0.0	3.8505946195589424	0.0
26	8.659508139937652E-4	0.0	0.0	3.9239117884770813	0.0
27	8.659508139937652E-4	0.0	0.0	4.001125736058192	0.0
28	8.659508139937652E-4	0.0	0.0	4.076752107146981	0.0
29	8.659508139937652E-4	0.0	0.0	4.165800715852673	0.0
30	8.659508139937652E-4	0.0	0.0	4.28934303198245	0.0
31	0.0011546010853250202	0.0	0.0	4.37781434014548	0.0
32	0.0011546010853250202	0.0	0.0	4.460079667474887	0.0
33	0.0011546010853250202	0.0	0.0	4.546097448331602	0.0
34	0.0011546010853250202	0.0	0.0	4.636444983258285	0.0
35	0.0012989262209906478	0.0	0.0	4.741658007158526	0.0
36	0.0012989262209906478	0.0	0.0	4.843695878074126	0.0
37	0.0012989262209906478	0.0	0.0	4.932744486779818	0.0
38	0.0012989262209906478	0.0	0.0	5.027854751183466	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	55	1.8189894E-12	44.0	1
TCCGATG	20	7.8543153E-4	44.0	18
CCGATCT	20	7.8543153E-4	44.0	12
ACGCACC	25	4.4410048E-5	44.0	21
TATATCG	20	7.8543153E-4	44.0	2
TATTGCG	25	4.4410048E-5	44.0	1
CGGGCTA	20	7.8543153E-4	44.0	6
ACGTCGG	20	7.8543153E-4	44.0	2
CGCACGG	20	7.8543153E-4	44.0	2
CGTCCTA	25	4.4410048E-5	44.0	26
GTGACGG	50	2.7284841E-11	44.0	2
TCGAACG	25	4.4410048E-5	44.0	1
CGTTTTT	9970	0.0	43.42628	1
CGCGACC	135	0.0	40.74074	10
CGTTAGG	120	0.0	40.333332	2
GCGATGT	105	0.0	39.809525	9
CGTAAGG	50	1.3460522E-9	39.6	2
AGGGCGC	100	0.0	39.6	6
GCGCGAC	140	0.0	39.285713	9
CGACCCG	140	0.0	39.285713	32
>>END_MODULE