FastQCFastQC Report
Thu 26 May 2016
SRR1545986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545986_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences596747
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58290.9767958615627728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC207.853435E-444.06
AATCCGA207.853435E-444.012
TCGTTAG700.044.01
TAGACTG207.853435E-444.01
GCGTTAG351.444514E-744.01
ACGGATA207.853435E-444.042
ATGACGC408.300049E-944.026
CGGTCTA254.440259E-544.031
CGCACGG207.853435E-444.02
TAATGCG207.853435E-444.01
CGATACG254.440259E-544.010
AATCGTG254.440259E-544.01
CTTACGA207.853435E-444.023
AAGTCGC207.853435E-444.023
CGTTTTT87750.043.4233631
CGTTAGG1300.042.3076932
ACGTAGG1200.042.1666642
GCTCGAA900.041.55555721
TCGAATT850.041.41176623
AGGGTAT2050.040.780496
Produced by FastQC (version 0.11.5)