FastQCFastQC Report
Thu 26 May 2016
SRR1545985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545985_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences935317
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161291.724442087548927No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12320.13172004785543298No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG207.855729E-444.01
CACGGTA254.4422017E-544.017
CTATCCG207.855729E-444.01
TACGATG302.5272093E-644.01
ACGTCGC454.802132E-1044.020
TCATCCG207.855729E-444.035
CGAGAGT207.855729E-444.032
AATACGG207.855729E-444.02
CGTTTTT100700.043.169811
CGACGGT1200.042.16666428
CTTAACG557.8216544E-1140.0000041
GTTTTTT113550.039.195072
CGATTCG404.123449E-738.510
CGTTAGG1200.038.52
TCGACGG404.123449E-738.52
GCGTAAG404.123449E-738.51
CGGGTAT750.038.133336
TATACGG750.038.133332
CGCGACC2950.038.03389710
GGGCGAT12650.037.9130447
Produced by FastQC (version 0.11.5)