FastQCFastQC Report
Thu 26 May 2016
SRR1545984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545984_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences938172
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163951.747547358053747No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12600.13430373108555788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG207.855742E-444.01
CGCATCG207.855742E-444.021
ACCGAAC207.855742E-444.031
TCGGACG254.442213E-544.01
GTATCGC207.855742E-444.014
TGCTCGA207.855742E-444.031
CATCGTT254.442213E-544.036
GTATACG207.855742E-444.01
CGTTTTT104700.043.222541
TATACGG1000.041.82
ACGGGTA650.040.6153835
TACGGGT557.8216544E-1140.04
ATGCGAG557.8216544E-1140.01
GTTTTTT118600.039.3440132
AGTACGG1350.039.1111152
AATCGTT2650.039.01886722
ACACGTG800.038.542
TCTAGCG404.123467E-738.51
ATCGTTG2700.038.296323
TTATACG357.288778E-637.7142871
Produced by FastQC (version 0.11.5)