##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545984_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	938172
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.018816379086136	31.0	28.0	33.0	25.0	33.0
2	28.691266633410503	31.0	28.0	33.0	16.0	33.0
3	28.108002583747968	31.0	26.0	33.0	16.0	34.0
4	32.22248905318001	35.0	32.0	35.0	26.0	37.0
5	33.665924798437814	35.0	33.0	35.0	28.0	37.0
6	33.52910127354046	35.0	33.0	37.0	28.0	37.0
7	34.457307402054205	35.0	35.0	37.0	32.0	37.0
8	34.77416081486124	35.0	35.0	37.0	32.0	37.0
9	36.578565550879794	39.0	37.0	39.0	32.0	39.0
10	36.201320226994625	38.0	35.0	39.0	31.0	39.0
11	36.31726165351343	38.0	35.0	39.0	32.0	39.0
12	34.96283197537338	37.0	33.0	39.0	28.0	39.0
13	35.29707985316126	37.0	34.0	39.0	30.0	39.0
14	35.936815424037384	38.0	34.0	40.0	28.0	41.0
15	36.52592062009951	38.0	35.0	40.0	31.0	41.0
16	36.112194778782566	38.0	34.0	40.0	31.0	41.0
17	35.71041024460334	37.0	34.0	40.0	30.0	41.0
18	35.39361332463557	37.0	33.0	39.0	29.0	40.0
19	34.850916463079265	37.0	33.0	39.0	27.0	40.0
20	35.25173955308835	36.0	34.0	39.0	29.0	40.0
21	35.39748788068712	36.0	34.0	39.0	30.0	40.0
22	35.824652622333645	37.0	34.0	40.0	31.0	40.0
23	36.213994875140166	37.0	35.0	40.0	32.0	41.0
24	35.73891248086705	36.0	34.0	40.0	31.0	41.0
25	35.418880546424326	36.0	34.0	40.0	30.0	41.0
26	35.584318227361294	36.0	34.0	40.0	30.0	41.0
27	35.91336343442354	37.0	35.0	40.0	31.0	41.0
28	35.831690777384104	36.0	35.0	40.0	31.0	41.0
29	35.99465343241964	37.0	35.0	40.0	31.0	41.0
30	35.64409831033115	36.0	35.0	40.0	30.0	41.0
31	34.98214506508401	36.0	34.0	40.0	29.0	41.0
32	34.99704744972137	36.0	34.0	40.0	29.0	41.0
33	34.75081328370491	36.0	34.0	40.0	26.0	41.0
34	34.362130824624906	36.0	34.0	40.0	23.0	41.0
35	34.138865794331956	36.0	34.0	40.0	22.0	41.0
36	33.726360411523686	36.0	33.0	40.0	20.0	41.0
37	33.639380625301115	36.0	33.0	40.0	18.0	41.0
38	33.514309742776376	36.0	33.0	40.0	18.0	41.0
39	33.29310190455482	36.0	33.0	40.0	18.0	41.0
40	33.07805391761852	35.0	33.0	40.0	18.0	40.0
41	32.7701945911837	35.0	32.0	39.0	16.0	40.0
42	32.93102970457443	35.0	33.0	39.0	17.0	40.0
43	32.730617626618574	35.0	32.0	39.0	15.0	40.0
44	32.55642995101112	35.0	32.0	39.0	15.0	40.0
45	32.497976916812696	35.0	32.0	39.0	15.0	40.0
46	32.32146770528219	35.0	32.0	39.0	15.0	40.0
47	32.27322601825678	35.0	31.0	38.0	15.0	40.0
48	32.28368252303416	35.0	32.0	38.0	15.0	40.0
49	32.32762329295694	35.0	32.0	38.0	15.0	40.0
50	32.49317928908558	35.0	32.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	5.0
12	10.0
13	18.0
14	38.0
15	107.0
16	218.0
17	530.0
18	988.0
19	1701.0
20	2693.0
21	3951.0
22	5912.0
23	7938.0
24	11336.0
25	16888.0
26	24070.0
27	28047.0
28	27485.0
29	27043.0
30	30199.0
31	36675.0
32	48429.0
33	73960.0
34	117813.0
35	89408.0
36	93673.0
37	129349.0
38	134626.0
39	25059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.524986889397677	22.980647471892148	28.261235679598194	22.233129959111974
2	36.99545499119564	20.470233603219878	29.506316538971532	13.027994866612946
3	27.76868207535505	19.31181062747556	39.16254162349761	13.756965673671779
4	25.3642189278725	25.20753124160602	35.1662594918629	14.261990338658585
5	20.629586046055522	28.974857488818685	36.369024016918004	14.02653244820779
6	19.0468272342385	36.35218275540093	33.923843389058725	10.677146621301851
7	76.53607227672538	5.142234046635371	15.502274636207433	2.8194190404318182
8	78.08760014155187	3.6270534614121934	15.202969178359618	3.0823772186763194
9	74.21421658288672	5.200858691156846	16.726570394341337	3.8583543316150983
10	44.65950806461928	20.513082888851937	22.288130534699395	12.539278511829387
11	38.813138742149626	20.218893763616904	27.469909568821073	13.498057925412398
12	34.31396375078344	19.803404919353806	31.289251864263694	14.593379465599057
13	20.776574018410273	31.942756765283974	31.83797853698469	15.442690679321064
14	16.533642018734305	33.23761527736918	34.782321365378635	15.446421338517885
15	15.550879796028875	20.86046055520736	47.85316551762364	15.735494131140133
16	17.731290211176628	17.903326895281463	45.959909270368335	18.405473623173577
17	17.98444208524663	18.077708565167157	34.95073398054941	28.987115369036808
18	21.717339677585773	20.987622738687577	38.061037848070505	19.23399973565615
19	25.513551886008106	22.617387856384543	32.18397052992415	19.6850897276832
20	27.16676686151367	21.2837304886524	32.0167304076438	19.53277224219013
21	21.761787817159327	24.92400114264762	33.90391100992142	19.410300030271635
22	21.929454300490743	21.77127435054553	32.73109834870365	23.56817300026008
23	19.376724097500244	25.466545580128162	32.15327253424745	23.00345778812414
24	19.205753316023074	21.859104727064974	40.71076519017834	18.224376766733606
25	18.425725773099174	22.198807894501222	38.689813808128996	20.685652524270605
26	17.199724570761013	27.829012164080787	33.766729341741176	21.204533923417028
27	17.585261551186775	27.7957560020977	34.7526892723296	19.866293174385934
28	16.66218987563048	24.93700515470511	40.13901502069983	18.261789948964584
29	17.835855258950385	23.04545435165407	38.64312727303735	20.475563116358195
30	19.645757920722424	25.57494787736151	36.00597758193593	18.773316619980132
31	25.804969664411214	23.547281308757885	31.87912237841249	18.76862664841841
32	26.659930162059837	23.33356783191142	31.83019744780275	18.176304558225997
33	25.124390836648292	24.556371326366595	30.555271314854842	19.763966522130268
34	19.517423244351782	25.203374221358132	33.41743305065596	21.86176948363413
35	20.326336748485353	24.736082509390602	33.566872599054335	21.370708143069713
36	26.9749043885343	24.637273335806228	29.261691885922836	19.126130389736637
37	21.477937947412627	27.834661448007402	31.210481660079388	19.476918944500582
38	21.602861735374752	28.41429929693063	28.194296994580952	21.788541973113674
39	21.604353999053476	27.638215593729083	28.952793304426056	21.804637102791386
40	23.525217124365255	24.392648682757535	28.601045437297213	23.48108875558
41	19.56379000865513	23.669646930413613	29.897396213061146	26.869166847870112
42	22.447269797009504	24.258558132197507	27.55283679325326	25.74133527753973
43	21.839492118715974	24.91781890740717	29.074306203979656	24.168382769897203
44	20.766661123973	27.042376024865376	30.23752574154846	21.953437109613162
45	18.91017851737208	30.699914301428738	27.59398063468106	22.79592654651812
46	22.10223711643494	27.91780185296513	29.18004374464384	20.79991728595609
47	21.58985772331726	25.805715796250585	30.24882430940169	22.355602171030473
48	22.396106470881673	24.08108534469159	31.40170459148216	22.12110359294458
49	21.44681359068486	23.50912199468754	32.30036709686497	22.743697317762628
50	19.869384292006156	27.907249416951263	30.303291933675276	21.920074357367305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	381.0
1	821.5
2	1262.0
3	13485.0
4	25708.0
5	17915.5
6	10123.0
7	10219.0
8	10315.0
9	10703.5
10	11092.0
11	10995.5
12	10899.0
13	10331.5
14	9764.0
15	9165.5
16	8567.0
17	7896.5
18	7226.0
19	6689.5
20	6153.0
21	5915.5
22	5678.0
23	5627.0
24	5576.0
25	5873.5
26	6171.0
27	6799.0
28	7427.0
29	8362.0
30	9297.0
31	11066.5
32	12836.0
33	14852.0
34	16868.0
35	19085.0
36	21302.0
37	23348.5
38	25395.0
39	31501.5
40	37608.0
41	49232.0
42	60856.0
43	69832.5
44	78809.0
45	79472.0
46	80135.0
47	77504.5
48	74874.0
49	74040.0
50	73206.0
51	67314.0
52	61422.0
53	56534.0
54	51646.0
55	47476.0
56	43306.0
57	40731.5
58	38157.0
59	36400.5
60	34644.0
61	30533.5
62	26423.0
63	24031.5
64	21640.0
65	17804.5
66	13969.0
67	11681.5
68	9394.0
69	8447.0
70	7500.0
71	6159.5
72	4819.0
73	4262.0
74	3705.0
75	2784.0
76	1863.0
77	1435.0
78	1007.0
79	780.5
80	554.0
81	421.5
82	289.0
83	214.0
84	139.0
85	109.0
86	79.0
87	58.0
88	37.0
89	31.5
90	26.0
91	20.0
92	14.0
93	11.5
94	9.0
95	5.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	938172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.892009100659912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5394377598437	22.281335518753206
2	7.901044382233286	4.723561811568705
3	3.574638266680518	3.205593587975436
4	2.277175149468378	2.7227736116682135
5	1.6668459074469983	2.4912686517401696
6	1.2081199283947135	2.1667879136558024
7	0.9759544717943628	2.042126796689478
8	0.7701566976333021	1.8417224811671078
9	0.6070939534989783	1.6332532184652355
>10	5.623476950766927	36.126776854882706
>50	0.7744417421382004	15.590204506192201
>100	0.08089571706028402	3.27716471661433
>500	0.0	0.0
>1k	3.5953652026790106E-4	0.13541558859193295
>5k	0.0	0.0
>10k+	3.5953652026790106E-4	1.7620147420355083
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16395	1.747547358053747	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1260	0.13430373108555788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01939942782346947	0.0
2	0.0	0.0	0.0	0.09518510465031998	0.0
3	0.0	0.0	0.0	0.15061204128880418	0.0
4	0.0	0.0	0.0	0.24281261858166733	0.0
5	0.0	0.0	0.0	0.42337652370780626	0.0
6	0.0	0.0	0.0	0.6800458764490946	0.0
7	0.0	0.0	0.0	0.8600768302614019	0.0
8	0.0	0.0	0.0	1.361370836051385	0.0
9	0.0	0.0	0.0	1.6440482129076543	0.0
10	2.1318052553263153E-4	0.0	0.0	2.0474923574781596	0.0
11	2.1318052553263153E-4	0.0	0.0	2.294142225519414	0.0
12	2.1318052553263153E-4	0.0	0.0	2.520859714423368	0.0
13	4.2636105106526307E-4	0.0	0.0	2.616471180124753	0.0
14	4.2636105106526307E-4	0.0	0.0	2.6577216118153175	0.0
15	5.329513138315788E-4	0.0	0.0	2.701210439023974	0.0
16	5.329513138315788E-4	0.0	0.0	2.8005525639221807	0.0
17	6.395415765978946E-4	0.0	0.0	2.918867755592791	0.0
18	6.395415765978946E-4	0.0	0.0	3.096766904149772	0.0
19	6.395415765978946E-4	0.0	0.0	3.1661571652106435	0.0
20	6.395415765978946E-4	0.0	0.0	3.2637938459045888	0.0
21	6.395415765978946E-4	0.0	0.0	3.3549285205697887	0.0
22	6.395415765978946E-4	0.0	0.0	3.450753166796707	0.0
23	6.395415765978946E-4	0.0	0.0	3.5509480137970435	0.0
24	6.395415765978946E-4	0.0	0.0	3.6349411408569003	0.0
25	6.395415765978946E-4	0.0	0.0	3.7009205135092498	0.0
26	6.395415765978946E-4	0.0	0.0	3.7706305453584203	0.0
27	7.461318393642104E-4	0.0	0.0	3.854197311367212	0.0
28	7.461318393642104E-4	0.0	0.0	3.93179502266109	0.0
29	7.461318393642104E-4	0.0	0.0	4.014295886042218	0.0
30	7.461318393642104E-4	0.0	0.0	4.13516924401922	0.0
31	8.527221021305261E-4	0.0	0.0	4.227263231049317	0.0
32	8.527221021305261E-4	0.0	0.0	4.315306788094294	0.0
33	8.527221021305261E-4	0.0	0.0	4.402923984088206	0.0
34	8.527221021305261E-4	0.0	0.0	4.500667255044917	0.0
35	8.527221021305261E-4	0.0	0.0	4.621114251970854	0.0
36	8.527221021305261E-4	0.0	0.0	4.721095918445658	0.0
37	8.527221021305261E-4	0.0	0.0	4.818626008876837	0.0
38	9.593123648968419E-4	0.0	0.0	4.942590484474063	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	7.855742E-4	44.0	1
CGCATCG	20	7.855742E-4	44.0	21
ACCGAAC	20	7.855742E-4	44.0	31
TCGGACG	25	4.442213E-5	44.0	1
GTATCGC	20	7.855742E-4	44.0	14
TGCTCGA	20	7.855742E-4	44.0	31
CATCGTT	25	4.442213E-5	44.0	36
GTATACG	20	7.855742E-4	44.0	1
CGTTTTT	10470	0.0	43.22254	1
TATACGG	100	0.0	41.8	2
ACGGGTA	65	0.0	40.615383	5
TACGGGT	55	7.8216544E-11	40.0	4
ATGCGAG	55	7.8216544E-11	40.0	1
GTTTTTT	11860	0.0	39.344013	2
AGTACGG	135	0.0	39.111115	2
AATCGTT	265	0.0	39.018867	22
ACACGTG	80	0.0	38.5	42
TCTAGCG	40	4.123467E-7	38.5	1
ATCGTTG	270	0.0	38.2963	23
TTATACG	35	7.288778E-6	37.714287	1
>>END_MODULE