FastQCFastQC Report
Thu 26 May 2016
SRR1545983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545983_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences923469
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164081.7767786466031887No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13340.14445530927405253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG254.442158E-544.01
ACTCGAT207.8556774E-444.020
TACAATC351.4456782E-744.025
CGTTTTT100750.043.1265531
CGTAAGG1450.042.4827582
ACACGTG750.041.06666642
CTAACGG750.041.0666662
GCGTTAG650.040.6153831
TCACGAC1400.039.28571325
GTTTTTT114250.039.1282272
GTCGATG452.3501343E-839.111111
ATACCGG452.3501343E-839.111112
CTTAACG404.123358E-738.51
CGGGATA1150.038.260876
ACGGGTA750.038.133335
GTTGATC3350.038.08955416
ATGCGAG1100.038.01
CTAACAC3200.037.12528
CACTAGG950.037.052632
GCGTAAG950.037.052631
Produced by FastQC (version 0.11.5)