FastQCFastQC Report
Thu 26 May 2016
SRR1545982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545982_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences797314
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85401.07109620551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTG650.044.00000442
TGACGTT207.8550295E-444.037
ATACTCG254.4416087E-544.044
TAATTCG254.4416087E-544.025
AACGCAT207.8550295E-444.024
TATACGG502.7284841E-1144.02
TCGACGG551.8189894E-1244.02
GCCGTTG207.8550295E-444.01
TACGTAG207.8550295E-444.01
CGTTTTT90650.043.3447341
ATAGGGT1500.041.0666664
ACGGCGG1650.040.05
GCGTAAG557.8216544E-1140.01
CGGGAAT11750.039.6936159
ATTTACG501.3460522E-939.6000021
CGTAGGC501.3460522E-939.6000023
CGTAAGG1450.039.4482772
GTTTTTT104000.038.8596152
CTAGCGG750.038.133332
TGGGATC2200.038.06
Produced by FastQC (version 0.11.5)