##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545982_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	797314
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.821479366974618	28.0	25.0	30.0	16.0	33.0
2	25.973386896505016	30.0	25.0	31.0	10.0	33.0
3	26.188013756186397	30.0	25.0	31.0	16.0	33.0
4	28.20946954399396	32.0	19.0	35.0	10.0	37.0
5	31.123375483184795	33.0	30.0	35.0	19.0	37.0
6	29.70180255206857	33.0	28.0	35.0	17.0	37.0
7	29.57916830759274	32.0	28.0	35.0	17.0	35.0
8	32.30071339522446	33.0	32.0	35.0	28.0	35.0
9	26.885074136413007	30.0	23.0	34.0	10.0	35.0
10	31.097024509791627	34.0	28.0	37.0	17.0	37.0
11	33.429856994860245	35.0	32.0	37.0	27.0	39.0
12	32.885607176093735	35.0	31.0	37.0	23.0	39.0
13	33.108610911134136	35.0	31.0	37.0	23.0	39.0
14	32.784512751563376	35.0	30.0	38.0	21.0	40.0
15	31.72962220655852	34.0	27.0	38.0	18.0	40.0
16	30.28550357826402	33.0	26.0	37.0	17.0	40.0
17	30.402546550041766	32.0	26.0	37.0	17.0	40.0
18	31.353493103093637	34.0	27.0	37.0	17.0	39.0
19	30.985286850600893	34.0	27.0	37.0	18.0	39.0
20	31.73960070938175	34.0	29.0	37.0	19.0	39.0
21	32.62418068665544	34.0	31.0	37.0	23.0	39.0
22	33.067081476055854	35.0	31.0	37.0	25.0	39.0
23	34.37902883932804	35.0	33.0	38.0	28.0	40.0
24	34.00944044629845	35.0	33.0	38.0	27.0	40.0
25	32.77109896477423	35.0	31.0	38.0	23.0	40.0
26	26.504498854905346	33.0	20.0	36.0	0.0	39.0
27	29.722292597395757	34.0	24.0	37.0	15.0	39.0
28	31.180820856024102	34.0	27.0	37.0	19.0	39.0
29	32.52094281550305	34.0	30.0	37.0	23.0	39.0
30	32.270247355496075	35.0	30.0	37.0	22.0	39.0
31	31.17784963013317	34.0	29.0	36.0	19.0	39.0
32	31.527312953240504	34.0	30.0	37.0	19.0	39.0
33	31.28971145621424	34.0	30.0	37.0	18.0	39.0
34	31.289644983030524	34.0	30.0	37.0	17.0	39.0
35	30.506398984590763	34.0	29.0	37.0	13.0	39.0
36	30.256093584208983	34.0	27.0	37.0	11.0	39.0
37	30.356958488123876	34.0	28.0	37.0	10.0	39.0
38	30.11697273596099	34.0	27.0	37.0	10.0	39.0
39	29.988248042803715	34.0	27.0	37.0	10.0	39.0
40	29.875690129610167	33.0	27.0	36.0	10.0	39.0
41	29.285218872363963	33.0	26.0	36.0	10.0	38.0
42	29.4628791166341	33.0	26.0	36.0	10.0	38.0
43	29.016314776863318	33.0	26.0	36.0	10.0	38.0
44	28.785753166255702	33.0	25.0	35.0	10.0	38.0
45	28.2763541089207	32.0	24.0	35.0	10.0	38.0
46	27.853785334259776	31.0	23.0	35.0	8.0	38.0
47	27.920824167141177	31.0	23.0	35.0	9.0	38.0
48	28.011769516150476	32.0	23.0	35.0	9.0	38.0
49	27.849426950987944	31.0	24.0	35.0	8.0	38.0
50	29.69281989279004	34.0	26.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	9.0
11	17.0
12	58.0
13	136.0
14	314.0
15	627.0
16	1199.0
17	2192.0
18	3754.0
19	5920.0
20	8352.0
21	11906.0
22	15331.0
23	20539.0
24	26237.0
25	32438.0
26	38683.0
27	43237.0
28	49017.0
29	57070.0
30	68271.0
31	79787.0
32	87063.0
33	85377.0
34	72917.0
35	54985.0
36	26658.0
37	5082.0
38	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.670706898411414	23.84455810383362	27.990101766681637	23.49463323107333
2	36.95470040661521	17.570116666708476	31.264219617365303	14.210963309311012
3	29.24556699117286	14.919968795229984	40.824693909802164	15.009770303794992
4	26.661265197901958	25.13677171101975	33.98347451568642	14.218488575391877
5	19.95086001249194	29.17909380745854	37.05804739412578	13.811998785923741
6	19.04318750203809	34.71003895579408	35.24194984660999	11.004823695557835
7	79.17558201662081	3.5980805554649735	15.266507298253886	1.9598301296603344
8	81.34198070020092	2.0979187622442352	14.638774686008274	1.9213258515465677
9	68.73126522298617	3.210529352300348	14.94053785585102	13.117667568862457
10	46.10818322517854	20.299028989833364	22.158898501719523	11.433889283268574
11	40.08195014762064	19.632917520575333	27.553260070687337	12.7318722611167
12	35.23367205392104	19.90984731235122	31.636594867266847	13.219885766460893
13	20.206091953734663	33.01033219032903	31.97146920786541	14.812106648070896
14	15.808577298278973	33.76662143145611	35.36310663051194	15.06169463975297
15	14.005147281999314	19.078054568212774	48.49268920400244	18.424108945785473
16	15.755147909104819	15.920954605086578	46.38097412060995	21.942923365198656
17	17.049242832811164	16.95304484807742	35.67741191048947	30.320300408621947
18	21.342532553046855	20.41654856179623	39.32428127437873	18.916637610778185
19	26.18290911736154	21.836440850154393	32.988508918694514	18.992141113789547
20	27.956865174824475	20.746531479442226	32.616008247691624	18.680595098041675
21	21.58860875389119	24.540018110807036	34.509365193637635	19.362007941664135
22	21.567412587763414	21.37313530177571	32.900839568852426	24.158612541608452
23	18.65877182640716	25.436778985443624	32.65840057994717	23.246048608202038
24	18.461609855088458	21.083788820966394	42.97629290342324	17.478308420521902
25	17.744828260885924	20.807987819102635	40.686981540522304	20.760202379489133
26	13.653215671617454	39.925675455341306	28.283712564936774	18.13739630810446
27	16.625946615762423	28.738865741727853	35.38492990214645	19.25025774036327
28	15.992444632854811	24.771796306097723	41.6173552703201	17.618403790727367
29	17.0104877124947	22.703978608176953	40.05209992549987	20.23343375382848
30	19.13700248584623	25.58603009604748	36.728189897581125	18.548777520525164
31	26.63154039688253	23.15235904549525	32.28364232912002	17.932458228502195
32	27.094720524159865	22.434699503583282	32.44957444620313	18.021005526053727
33	25.62052089891812	24.250797051099067	30.729298620117042	19.399383429865775
34	19.37919063254878	24.580027442136977	33.334044052907636	22.706737872406606
35	20.12406655345322	24.349628878961113	33.81001713252245	21.716287435063226
36	27.41191048946839	24.166639492094706	29.102085251231006	19.319364767205894
37	20.71806088943628	28.47397637568135	30.988418615501544	19.819544119380822
38	20.982950255482784	29.273535896773417	27.888636095691282	21.854877752052516
39	21.047542122676887	28.0876793835302	28.8264096704686	22.038368823324312
40	23.508429552221585	23.966843677647702	28.629623962453937	23.895102807676775
41	18.848157689442303	23.10620408019927	30.039607983805627	28.0060302465528
42	21.787777462831457	23.540788196369313	26.878243703233608	27.79319063756563
43	21.335007286965986	24.102047624900607	29.124284786169564	25.438660301963843
44	19.80725285144874	26.7094269008195	30.467670202705584	23.015650045026177
45	18.138775940219286	31.882796489212534	27.0055461211016	22.972881449466584
46	21.313560278635517	27.90055110031932	28.96274240763363	21.82314621341153
47	20.99774994544182	25.553671451899753	29.646789094384395	23.80178950827403
48	21.531291310575256	23.578414526773642	32.13138111208382	22.75891305056728
49	21.088053138412217	23.295840785437104	32.21390819677066	23.40219787938002
50	19.410044223480334	28.67239255801353	30.160764767707576	21.756798450798556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	206.0
1	471.5
2	737.0
3	8049.0
4	15361.0
5	12157.5
6	8954.0
7	8685.5
8	8417.0
9	8961.5
10	9506.0
11	9744.5
12	9983.0
13	9996.5
14	10010.0
15	9450.0
16	8890.0
17	8515.5
18	8141.0
19	7812.0
20	7483.0
21	6953.5
22	6424.0
23	6372.5
24	6321.0
25	6324.0
26	6327.0
27	6665.0
28	7003.0
29	7873.5
30	8744.0
31	10134.5
32	11525.0
33	13336.0
34	15147.0
35	16306.0
36	17465.0
37	19261.5
38	21058.0
39	25750.0
40	30442.0
41	39802.0
42	49162.0
43	56909.0
44	64656.0
45	65738.0
46	66820.0
47	64779.5
48	62739.0
49	60740.5
50	58742.0
51	54920.5
52	51099.0
53	47319.5
54	43540.0
55	40469.5
56	37399.0
57	35457.5
58	33516.0
59	31563.0
60	29610.0
61	26604.5
62	23599.0
63	21107.0
64	18615.0
65	15703.5
66	12792.0
67	10685.0
68	8578.0
69	7677.5
70	6777.0
71	5610.5
72	4444.0
73	3803.0
74	3162.0
75	2462.5
76	1763.0
77	1367.0
78	971.0
79	779.0
80	587.0
81	423.0
82	259.0
83	209.5
84	160.0
85	134.0
86	108.0
87	71.0
88	34.0
89	26.0
90	18.0
91	14.5
92	11.0
93	8.5
94	6.0
95	3.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	797314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.12423814264293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24213281570262	37.47231481207693
2	7.3536920112355215	6.783668831077561
3	2.9648751184164315	4.102578180751084
4	1.7172337686698507	3.1682439717086557
5	1.1212534222323012	2.5858479932648013
6	0.7875439744606033	2.1794919495494622
7	0.5814218586235808	1.8772348187944476
8	0.44474019085978533	1.6410641979856966
9	0.39103229450060717	1.623246000570908
>10	3.1533391209435857	29.508087996388916
>50	0.22198881841417145	6.570772438071325
>100	0.02015384577120879	1.2448180293300395
>500	2.963800848702769E-4	0.07518668088411355
>1k	0.0	0.0
>5k	2.963800848702769E-4	1.1674440995460542
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8540	1.07109620551	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01630474317521077	0.0
2	0.0	0.0	0.0	0.08503550671379155	0.0
3	0.0	0.0	0.0	0.13093962980707727	0.0
4	0.0	0.0	0.0	0.1908909162513138	0.0
5	0.0	0.0	0.0	0.32145428275434773	0.0
6	0.0	0.0	0.0	0.4906473484724964	0.0
7	0.0	0.0	0.0	0.5986349167329308	0.0
8	0.0	0.0	0.0	0.916451987548193	0.0
9	0.0	0.0	0.0	1.1430879176836228	0.0
10	0.0	0.0	0.0	1.4136212332907738	0.0
11	0.0	0.0	0.0	1.5914683550019189	0.0
12	0.0	0.0	0.0	1.741471992213858	0.0
13	0.0	0.0	0.0	1.812836598880742	0.0
14	0.0	0.0	0.0	1.8359140815287327	0.0
15	0.0	0.0	0.0	1.8648863559400688	0.0
16	0.0	0.0	0.0	1.919444535026351	0.0
17	0.0	0.0	0.0	2.001971619713187	0.0
18	0.0	0.0	0.0	2.1222504559057036	0.0
19	0.0	0.0	0.0	2.1632631560464257	0.0
20	0.0	0.0	0.0	2.2259737067203136	0.0
21	0.0	0.0	0.0	2.2811589913133345	0.0
22	0.0	0.0	0.0	2.3340866960820956	0.0
23	0.0	0.0	0.0	2.3932854559182455	0.0
24	0.0	0.0	0.0	2.4408200533290523	0.0
25	0.0	0.0	0.0	2.477192172719907	0.0
26	0.0	0.0	0.0	2.525980981144192	0.0
27	0.0	0.0	0.0	2.5849288987776458	0.0
28	0.0	0.0	0.0	2.636602392532929	0.0
29	0.0	0.0	0.0	2.6955503101663836	0.0
30	0.0	0.0	0.0	2.777951973751872	0.0
31	0.0	0.0	0.0	2.844801420770236	0.0
32	0.0	0.0	0.0	2.902745969592908	0.0
33	0.0	0.0	0.0	2.9704733643207066	0.0
34	0.0	0.0	0.0	3.0397058122646787	0.0
35	0.0	0.0	0.0	3.125493845586557	0.0
36	0.0	0.0	0.0	3.196231346746702	0.0
37	0.0	0.0	0.0	3.264460425879892	0.0
38	0.0	0.0	0.0	3.3531331445327686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTG	65	0.0	44.000004	42
TGACGTT	20	7.8550295E-4	44.0	37
ATACTCG	25	4.4416087E-5	44.0	44
TAATTCG	25	4.4416087E-5	44.0	25
AACGCAT	20	7.8550295E-4	44.0	24
TATACGG	50	2.7284841E-11	44.0	2
TCGACGG	55	1.8189894E-12	44.0	2
GCCGTTG	20	7.8550295E-4	44.0	1
TACGTAG	20	7.8550295E-4	44.0	1
CGTTTTT	9065	0.0	43.344734	1
ATAGGGT	150	0.0	41.066666	4
ACGGCGG	165	0.0	40.0	5
GCGTAAG	55	7.8216544E-11	40.0	1
CGGGAAT	1175	0.0	39.693615	9
ATTTACG	50	1.3460522E-9	39.600002	1
CGTAGGC	50	1.3460522E-9	39.600002	3
CGTAAGG	145	0.0	39.448277	2
GTTTTTT	10400	0.0	38.859615	2
CTAGCGG	75	0.0	38.13333	2
TGGGATC	220	0.0	38.0	6
>>END_MODULE