FastQCFastQC Report
Thu 26 May 2016
SRR1545981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545981_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1241530
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209931.6908975216064048No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG650.044.0000042
TATTTCG207.856727E-444.038
ACTATCG207.856727E-444.012
CGACGTC302.5278823E-644.018
CGTTCGA207.856727E-444.014
TGCGCTA351.4462057E-744.022
ACGCTAT207.856727E-444.012
CGTAAGT254.443046E-544.030
TCGGTAA207.856727E-444.012
ATACCCG408.312782E-944.011
TACGTCG207.856727E-444.01
ACGGTCT950.044.030
CGTTTTT176650.043.0285871
TTGTACG1050.041.9047621
CGACGGT1000.041.80000328
CTAGCGG1850.041.621622
TAACGGG2950.041.016953
GGCGATA4000.040.158
ATCCGGG557.8216544E-1140.03
TAGGGTA4100.039.707325
Produced by FastQC (version 0.11.5)