##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545981_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1241530
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.122004301144557	31.0	28.0	33.0	25.0	33.0
2	28.81375721891537	31.0	28.0	33.0	16.0	33.0
3	28.1785281064493	31.0	27.0	33.0	16.0	34.0
4	32.08701682601306	35.0	32.0	35.0	25.0	37.0
5	33.63517756316803	35.0	33.0	35.0	28.0	37.0
6	33.47403445748391	35.0	33.0	37.0	28.0	37.0
7	34.30948909812892	35.0	35.0	37.0	31.0	37.0
8	34.77711533349979	35.0	35.0	37.0	32.0	37.0
9	36.60167454672863	39.0	37.0	39.0	32.0	39.0
10	36.33338541960323	39.0	35.0	39.0	32.0	39.0
11	36.33446392757324	38.0	35.0	39.0	31.0	39.0
12	35.14894203120343	37.0	34.0	39.0	29.0	39.0
13	35.17392249885222	37.0	34.0	39.0	27.0	39.0
14	35.91750743034804	38.0	34.0	40.0	27.0	41.0
15	36.579263489404205	38.0	35.0	40.0	31.0	41.0
16	36.231826858795195	38.0	34.0	40.0	31.0	41.0
17	35.84164377824136	37.0	34.0	40.0	30.0	41.0
18	35.46037147712902	37.0	34.0	39.0	29.0	40.0
19	34.8927387980959	37.0	33.0	39.0	27.0	40.0
20	35.26855170636231	35.0	34.0	39.0	30.0	40.0
21	35.42588982948459	35.0	34.0	39.0	30.0	40.0
22	35.763578810016675	36.0	34.0	39.0	31.0	41.0
23	36.098525206801284	36.0	35.0	40.0	32.0	41.0
24	35.766446239720345	36.0	35.0	40.0	31.0	41.0
25	35.394497917891634	35.0	34.0	40.0	30.0	41.0
26	35.48819601620581	35.0	34.0	40.0	30.0	41.0
27	35.76941837893567	36.0	35.0	40.0	31.0	41.0
28	35.73012331558641	36.0	35.0	40.0	31.0	41.0
29	35.899614991180236	36.0	35.0	40.0	31.0	41.0
30	35.523548363712514	36.0	35.0	40.0	30.0	41.0
31	34.806589450113975	35.0	34.0	39.0	29.0	41.0
32	34.67359467753498	35.0	34.0	40.0	27.0	41.0
33	34.25674611165256	36.0	34.0	40.0	23.0	41.0
34	33.83613605792852	36.0	34.0	40.0	21.0	41.0
35	33.482919462276385	36.0	33.0	40.0	18.0	41.0
36	33.030065322626115	35.0	33.0	40.0	15.0	41.0
37	32.89856628514816	35.0	33.0	40.0	15.0	41.0
38	32.756982916240446	35.0	33.0	40.0	15.0	41.0
39	32.59122373200809	35.0	32.0	40.0	12.0	40.0
40	32.41345436678937	35.0	32.0	39.0	12.0	40.0
41	32.16548452312872	35.0	31.0	39.0	10.0	40.0
42	32.317043486665646	35.0	32.0	39.0	10.0	40.0
43	32.08424444032766	35.0	31.0	39.0	10.0	40.0
44	31.906248741472215	35.0	31.0	39.0	10.0	40.0
45	31.846989601540034	35.0	31.0	39.0	10.0	40.0
46	31.683728947347223	35.0	31.0	39.0	10.0	40.0
47	31.661008594234534	35.0	31.0	39.0	10.0	40.0
48	31.674428326339275	35.0	31.0	38.0	10.0	40.0
49	31.67979267516693	35.0	31.0	39.0	10.0	40.0
50	31.78422349842533	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	5.0
13	20.0
14	36.0
15	133.0
16	306.0
17	740.0
18	1590.0
19	2531.0
20	4104.0
21	6135.0
22	8597.0
23	12591.0
24	17755.0
25	27770.0
26	39204.0
27	43359.0
28	39623.0
29	36921.0
30	39292.0
31	47218.0
32	63349.0
33	96902.0
34	157289.0
35	111035.0
36	111429.0
37	157511.0
38	177192.0
39	38892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.766191715061254	22.256006701408744	27.680603771153333	24.297197812376663
2	38.46858311921581	20.168904496870795	29.265986323326864	12.096526060586536
3	26.058250706789206	18.73849202194067	42.48975054972494	12.71350672154519
4	23.760279654941886	24.533841308707803	38.04821470282635	13.65766433352396
5	19.739192770210952	29.291841518126827	38.49210248644817	12.47686322521405
6	17.966460737960418	35.87339814583619	35.74911601008433	10.411025106119062
7	75.21848042334861	4.552447383470396	17.869483621016006	2.35958857216499
8	76.60080706869749	3.2046748769663234	17.47432603320097	2.720192021135212
9	73.15401158248291	4.8456340160930464	18.85576667498973	3.144587726434319
10	44.0168985042649	23.637930617866665	23.469267758330446	8.87590311953799
11	37.962111265938	18.92632477668683	31.910304221404235	11.20125973597094
12	34.74229378267138	19.567952445772555	33.30825674772257	12.381497023833496
13	19.060111314265463	34.65675416623037	33.42408157676415	12.859052942740007
14	13.771636609667103	35.53277004985784	36.841961128607444	13.853632211867614
15	12.783984277464096	21.214871972485565	52.390115422100145	13.611028327950189
16	14.75526165296046	17.52587533124451	50.63542564416487	17.08343737163017
17	15.22113843402898	18.11635643117766	37.28858746868783	29.37391766610553
18	19.2204779586478	20.82607750114778	41.69742172964004	18.256022810564385
19	24.712894573630923	21.93084339484346	34.92585761117331	18.430404420352307
20	26.26098443050108	21.42888210514446	34.732547743510025	17.57758572084444
21	19.833350784918608	25.139787198054016	37.035915362496276	17.990946654531104
22	20.040192343318324	22.006153697453946	34.73198392306267	23.221670036165055
23	16.658880574774674	26.52694659009448	34.92956271697019	21.88461011816066
24	16.652275820962846	21.203998292429503	45.93002182790589	16.213704058701765
25	15.056261226067836	22.623214904190796	43.033595644084315	19.28692822565705
26	14.433722906413859	29.83262587291487	36.77381940025614	18.959831820415133
27	15.264794245809608	30.171965236442134	37.33167946002111	17.23156105772716
28	13.36190023599913	25.10724670366403	45.02444564368159	16.506407416655257
29	14.238882668964905	21.52924214477298	43.798216716470804	20.433658469791304
30	17.46321071581033	26.905028472932592	38.29202677341667	17.339734037840408
31	24.457564456759	24.16316963746345	33.79467270223031	17.584593203547236
32	26.042947008932526	23.05332935974161	34.12885713595322	16.774866495372645
33	24.560743598624278	24.89718331413659	32.31657712660991	18.225495960629225
34	18.39045371436856	25.716897698807117	33.5901669713982	22.302481615426128
35	18.686137266115196	24.85932679838586	35.55145667039862	20.90307926510032
36	27.19515436598391	23.892374731178464	30.629706893913156	18.28276400892447
37	18.99503032548549	29.871207300669333	32.849145812022265	18.28461656182291
38	19.734601660853947	29.450436155388914	29.89311575233784	20.921846431419297
39	19.502871456992583	28.797854260468934	31.779578423396938	19.91969585914154
40	22.691034449429335	25.013813600960106	29.918084943577682	22.377067006032878
41	17.114447496234487	23.200647588056672	32.225963126142744	27.4589417895661
42	21.16646395979155	23.365846979130588	28.982545729865567	26.485143331212296
43	21.09220075229757	24.57218109912769	29.865085821526666	24.470532327048076
44	19.32607347385887	28.188767085773197	30.4961619936691	21.988997446698832
45	16.995884110734334	33.79120923376801	27.174856829879264	22.03804982561839
46	20.53136049873946	29.65510297777742	29.32083799827632	20.4926985252068
47	19.962626758918432	25.780609409357808	30.765990350615773	23.490773481107986
48	20.923940621652314	23.044791507253148	33.821252809034014	22.210015062060524
49	20.60151587154559	23.170523467012476	33.57679637221815	22.65116428922378
50	19.130266687071597	29.42353386547244	30.698976263159167	20.747223184296796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	454.0
1	762.0
2	1070.0
3	16752.0
4	32434.0
5	23983.0
6	15532.0
7	16033.5
8	16535.0
9	17593.5
10	18652.0
11	19121.5
12	19591.0
13	18558.5
14	17526.0
15	16568.5
16	15611.0
17	14539.5
18	13468.0
19	12426.0
20	11384.0
21	10780.0
22	10176.0
23	9885.5
24	9595.0
25	9395.0
26	9195.0
27	9886.0
28	10577.0
29	13009.5
30	15442.0
31	18266.0
32	21090.0
33	23690.5
34	26291.0
35	30437.5
36	34584.0
37	37305.5
38	40027.0
39	47912.5
40	55798.0
41	73675.0
42	91552.0
43	105248.5
44	118945.0
45	120446.0
46	121947.0
47	115142.0
48	108337.0
49	101309.5
50	94282.0
51	85655.0
52	77028.0
53	68196.5
54	59365.0
55	52186.5
56	45008.0
57	40636.5
58	36265.0
59	31571.5
60	26878.0
61	23516.0
62	20154.0
63	17915.5
64	15677.0
65	13267.0
66	10857.0
67	8640.0
68	6423.0
69	5603.5
70	4784.0
71	4026.0
72	3268.0
73	2756.5
74	2245.0
75	1876.0
76	1507.0
77	1246.0
78	985.0
79	765.0
80	545.0
81	395.5
82	246.0
83	167.0
84	88.0
85	61.0
86	34.0
87	36.0
88	38.0
89	29.5
90	21.0
91	17.0
92	13.0
93	9.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1241530.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.505340015856284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17003629504941	22.2826346364221
2	7.2151121123447375	4.113384480298196
3	2.9506520616805574	2.523280208600749
4	1.7585726401169424	2.00514844196462
5	1.26596408881485	1.804336839976549
6	0.9684135012525593	1.656297367749004
7	0.7354924850711135	1.4675824356241434
8	0.6391495818460892	1.457534092121211
9	0.49816429185726924	1.278030827083475
>10	4.5515907828354445	28.169207683532356
>50	0.9082474183994339	18.125586222220928
>100	0.3374694356495635	13.224836480791494
>500	5.676525410024018E-4	0.09870488502204341
>1k	2.838262705012009E-4	0.09498322542285162
>5k	0.0	0.0
>10k+	2.838262705012009E-4	1.698452173170293
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20993	1.6908975216064048	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04357526600243248	0.0
2	0.0	0.0	0.0	0.16270247194993273	0.0
3	0.0	0.0	0.0	0.22270907670374457	0.0
4	0.0	0.0	0.0	0.35351542048923507	0.0
5	0.0	0.0	0.0	0.6104564529250199	0.0
6	0.0	0.0	0.0	0.9178191425096454	0.0
7	0.0	0.0	0.0	1.1352927436308426	0.0
8	0.0	0.0	0.0	1.7596030704050647	0.0
9	0.0	0.0	0.0	2.0759063413691172	0.0
10	0.0	0.0	0.0	2.567396679903023	0.0
11	0.0	0.0	0.0	2.901258930513157	0.0
12	0.0	0.0	0.0	3.21385709568033	0.0
13	8.054577819303601E-5	0.0	0.0	3.3312928402857764	0.0
14	8.054577819303601E-5	0.0	0.0	3.376881750743035	0.0
15	1.6109155638607201E-4	0.0	0.0	3.4298003270158595	0.0
16	1.6109155638607201E-4	0.0	0.0	3.5657616006057045	0.0
17	1.6109155638607201E-4	0.0	0.0	3.735229917923852	0.0
18	1.6109155638607201E-4	0.0	0.0	4.002480809968345	0.0
19	1.6109155638607201E-4	0.0	0.0	4.1079957794012225	0.0
20	2.4163733457910805E-4	0.0	0.0	4.2267202564577575	0.0
21	2.4163733457910805E-4	0.0	0.0	4.37669649545319	0.0
22	2.4163733457910805E-4	0.0	0.0	4.525303456219342	0.0
23	2.4163733457910805E-4	0.0	0.0	4.682609361030342	0.0
24	2.4163733457910805E-4	0.0	0.0	4.803991848767247	0.0
25	2.4163733457910805E-4	0.0	0.0	4.9032242475010674	0.0
26	2.4163733457910805E-4	0.0	0.0	4.999556998219938	0.0
27	2.4163733457910805E-4	0.0	0.0	5.09854775961918	0.0
28	3.2218311277214403E-4	0.0	0.0	5.201807447262651	0.0
29	4.0272889096518004E-4	0.0	0.0	5.328103227469332	0.0
30	4.0272889096518004E-4	0.0	0.0	5.495235717219882	0.0
31	4.832746691582161E-4	0.0	0.0	5.636754649505046	0.0
32	4.832746691582161E-4	0.0	0.0	5.75942586969304	0.0
33	4.832746691582161E-4	0.0	0.0	5.875170152956433	0.0
34	4.832746691582161E-4	0.0	0.0	6.010084331429768	0.0
35	5.638204473512521E-4	0.0	0.0	6.175203176725492	0.0
36	5.638204473512521E-4	0.0	0.0	6.310197900977021	0.0
37	6.443662255442881E-4	0.0	0.0	6.4509113754802545	0.0
38	6.443662255442881E-4	0.0	0.0	6.604109445603409	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGG	65	0.0	44.000004	2
TATTTCG	20	7.856727E-4	44.0	38
ACTATCG	20	7.856727E-4	44.0	12
CGACGTC	30	2.5278823E-6	44.0	18
CGTTCGA	20	7.856727E-4	44.0	14
TGCGCTA	35	1.4462057E-7	44.0	22
ACGCTAT	20	7.856727E-4	44.0	12
CGTAAGT	25	4.443046E-5	44.0	30
TCGGTAA	20	7.856727E-4	44.0	12
ATACCCG	40	8.312782E-9	44.0	11
TACGTCG	20	7.856727E-4	44.0	1
ACGGTCT	95	0.0	44.0	30
CGTTTTT	17665	0.0	43.028587	1
TTGTACG	105	0.0	41.904762	1
CGACGGT	100	0.0	41.800003	28
CTAGCGG	185	0.0	41.62162	2
TAACGGG	295	0.0	41.01695	3
GGCGATA	400	0.0	40.15	8
ATCCGGG	55	7.8216544E-11	40.0	3
TAGGGTA	410	0.0	39.70732	5
>>END_MODULE