FastQCFastQC Report
Thu 26 May 2016
SRR1545980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545980_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1241170
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218461.7601134413496942No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG207.856724E-444.0000041
CGTGCAT207.856724E-444.00000437
ATACCGG408.312782E-944.0000042
ATCGAGT207.856724E-444.00000412
ATCGTAG302.5278805E-644.01
ATCCGGG302.5278805E-644.03
ACGATGC254.4430453E-544.028
ACGCTAG254.4430453E-544.01
CGTTTTT175700.043.3238451
TATGGGC2350.041.191494
ACCCGCT2200.041.034
ATTAACG700.040.8571431
TACGCGG700.040.8571432
CGATGCG557.8216544E-1140.010
GTTTTTT194000.039.7020652
CTACGGG1900.039.368423
AGGGCGA8250.039.26
TGCGGAT452.3512257E-839.111114
TAACGGG3100.039.0322573
CGGTCTA850.038.82353231
Produced by FastQC (version 0.11.5)