##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545980_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1241170
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.108234971841085	31.0	28.0	33.0	25.0	33.0
2	28.772063456254987	31.0	28.0	33.0	16.0	33.0
3	28.172754739479686	31.0	27.0	33.0	16.0	34.0
4	32.07439996132681	35.0	32.0	35.0	25.0	37.0
5	33.67991169622211	35.0	33.0	35.0	28.0	37.0
6	33.56574844702982	35.0	33.0	37.0	28.0	37.0
7	34.37193212855612	35.0	35.0	37.0	31.0	37.0
8	34.81418983700863	35.0	35.0	37.0	32.0	37.0
9	36.71707018377821	39.0	37.0	39.0	32.0	39.0
10	36.33914935101557	39.0	35.0	39.0	32.0	39.0
11	36.391016540844525	39.0	35.0	39.0	32.0	39.0
12	35.098345915547426	37.0	34.0	39.0	29.0	39.0
13	35.17737860244769	37.0	34.0	39.0	27.0	39.0
14	35.927800381897725	38.0	34.0	40.0	27.0	41.0
15	36.61032332396046	38.0	35.0	40.0	31.0	41.0
16	36.26440777653343	38.0	34.0	40.0	31.0	41.0
17	35.886119548490534	37.0	34.0	40.0	30.0	41.0
18	35.52758848505845	37.0	34.0	39.0	29.0	40.0
19	34.96133567520968	37.0	33.0	39.0	27.0	40.0
20	35.2421175181482	35.0	34.0	39.0	30.0	40.0
21	35.333428942046616	35.0	34.0	39.0	30.0	40.0
22	35.72832569269318	36.0	34.0	39.0	31.0	40.0
23	36.11414874674702	36.0	35.0	40.0	32.0	41.0
24	35.67486242819275	35.0	34.0	40.0	31.0	41.0
25	35.31904815617522	35.0	34.0	39.0	30.0	41.0
26	35.456292852711556	35.0	34.0	40.0	30.0	41.0
27	35.791314646664034	36.0	35.0	40.0	31.0	41.0
28	35.74611133043822	36.0	35.0	40.0	31.0	41.0
29	35.92122191158342	36.0	35.0	40.0	31.0	41.0
30	35.572500140996	36.0	35.0	40.0	31.0	41.0
31	34.85235463312842	35.0	34.0	39.0	29.0	41.0
32	34.75343506530129	35.0	34.0	40.0	27.0	41.0
33	34.35227486967941	36.0	34.0	40.0	24.0	41.0
34	33.859046705930695	36.0	34.0	40.0	21.0	41.0
35	33.52924901504226	36.0	33.0	40.0	18.0	41.0
36	33.0666137595978	35.0	33.0	40.0	15.0	41.0
37	32.93560189176342	35.0	33.0	40.0	15.0	41.0
38	32.821955090761136	35.0	33.0	40.0	15.0	41.0
39	32.62270277238412	35.0	32.0	40.0	12.0	40.0
40	32.42170935488289	35.0	32.0	39.0	12.0	40.0
41	32.168669078369604	35.0	32.0	39.0	10.0	40.0
42	32.32435121699687	35.0	32.0	39.0	10.0	40.0
43	32.11499794548692	35.0	31.0	39.0	10.0	40.0
44	31.925583119153703	35.0	31.0	39.0	10.0	40.0
45	31.855415454772512	35.0	31.0	39.0	10.0	40.0
46	31.6746738964042	35.0	31.0	39.0	10.0	40.0
47	31.65884286600546	35.0	31.0	38.0	10.0	40.0
48	31.675747883045837	35.0	31.0	38.0	10.0	40.0
49	31.739721391912468	35.0	31.0	39.0	10.0	40.0
50	31.863574691621615	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	7.0
13	16.0
14	47.0
15	117.0
16	316.0
17	676.0
18	1415.0
19	2442.0
20	3891.0
21	5823.0
22	8441.0
23	12047.0
24	17457.0
25	27161.0
26	39021.0
27	43759.0
28	39312.0
29	37285.0
30	38960.0
31	46759.0
32	62735.0
33	98041.0
34	158074.0
35	111453.0
36	112207.0
37	161221.0
38	177949.0
39	34535.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.8528646357872	22.10317684120628	27.716751129982192	24.327207393024324
2	38.630324613066705	20.22245139666605	29.066284231813533	12.080939758453717
3	25.95470402926271	18.72185115657001	42.51722165376218	12.806223160405104
4	23.55035974121192	24.624265813708032	38.04523151542496	13.780142929655085
5	19.692306452782454	29.32201068346802	38.43478330929687	12.550899554452656
6	18.03266272952134	35.84496886002723	35.817011368305714	10.305357042145717
7	75.06731551681075	4.558924240837275	17.990444499947632	2.3833157424043443
8	76.51989654922372	3.2062489425300322	17.559641306186904	2.714213202059347
9	73.14179363020375	4.857835751750365	18.93076693764754	3.069603680398334
10	44.0094427032558	23.344344449189073	23.49782866166601	9.148384185889121
11	37.935738053610706	18.835211937123844	31.98989662979286	11.239153379472594
12	34.82302988309417	19.1666733807617	33.3179177711353	12.692378965008821
13	19.069345859149028	34.62611890393742	33.406060410741475	12.89847482617208
14	13.722535994263477	35.61051266144041	36.882779957620635	13.784171386675476
15	12.79139843857006	21.186461161645866	52.4752451316097	13.546895268174383
16	14.767034330510729	17.45232321116366	50.72334974258159	17.057292715744016
17	15.136121562719046	18.18034596388891	37.25049751444202	29.43303495895002
18	19.17601940104901	20.726089093355462	41.823763062271894	18.27412844332364
19	24.655123794484236	21.906427000330332	34.976836372132745	18.461612833052683
20	26.300184503331536	21.10822852630985	34.783712142575155	17.80787482778346
21	19.806634063021182	25.13314050452396	37.102008588670365	17.958216843784495
22	20.01329390816729	21.889024066002243	34.832134195960265	23.265547829870204
23	16.662262220324372	26.611906507569472	34.89062739189636	21.835203880209804
24	16.669674581241892	21.289509092227497	45.871798383783045	16.16901794274757
25	15.0688463304785	22.498529613187557	43.1915853589758	19.241038697358135
26	14.474568350830264	29.824842688753357	36.744765020102	18.95582394031438
27	15.172861090745023	30.182972517866208	37.35757390204404	17.28659248934473
28	13.495250449172957	24.92527212227173	45.039116317668004	16.540361110887307
29	14.302875512621155	21.372253599426347	44.00259432632113	20.322276561631362
30	17.359910407115866	26.893898498996915	38.38732808559666	17.358863008290566
31	24.42292353182884	24.13255234979898	33.91968868084147	17.52483543753072
32	26.025524303681202	23.04019594414947	34.23793678545244	16.696342966716887
33	24.563758389261743	24.88893543994779	32.45204121917224	18.09526495161823
34	18.289275441720314	25.602133470838	33.7772424406004	22.33134864684129
35	18.631049735330375	24.836243222121063	35.75062239660965	20.782084645938912
36	27.188862122030024	23.731640307129563	30.782326353360133	18.297171217480283
37	18.90554879669989	29.844501559012865	32.9501196451735	18.29982999911374
38	19.806070079038328	29.392992096167326	29.968578035240945	20.832359789553408
39	19.429731624193302	28.833681123456095	31.836090140754287	19.900497111596316
40	22.706559133720603	24.91737634651176	29.943279325152876	22.43278519461476
41	17.115705342539698	23.238718306114393	32.217826728006635	27.42774962333927
42	21.079948758026703	23.43949660401073	29.087071070038757	26.393483567923816
43	21.0490907772505	24.59936994932201	29.909118009619956	24.44242126380754
44	19.276327980856774	28.20242190836066	30.609263839764093	21.911986271018474
45	17.117800140190305	33.658564096779656	27.264838821434612	21.95879694159543
46	20.570510083227923	29.64130618690429	29.398551366855468	20.389632363012318
47	20.039881724501882	25.785186557844614	30.72890901326974	23.44602270438377
48	20.961914967329214	23.060982782374694	33.85136604977561	22.12573620052048
49	20.56196975434469	23.142599321607836	33.80479708661988	22.490633837427588
50	19.120265555886785	29.450115616716488	30.646728490053736	20.78289033734299
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	459.0
1	779.5
2	1100.0
3	17381.5
4	33663.0
5	24553.5
6	15444.0
7	16166.5
8	16889.0
9	17799.5
10	18710.0
11	18983.0
12	19256.0
13	18529.5
14	17803.0
15	16665.5
16	15528.0
17	14449.0
18	13370.0
19	12235.5
20	11101.0
21	10466.0
22	9831.0
23	9597.5
24	9364.0
25	9383.0
26	9402.0
27	10028.0
28	10654.0
29	12878.0
30	15102.0
31	18106.5
32	21111.0
33	23384.5
34	25658.0
35	30333.5
36	35009.0
37	37413.5
38	39818.0
39	47470.5
40	55123.0
41	73436.5
42	91750.0
43	105199.5
44	118649.0
45	120414.5
46	122180.0
47	114938.5
48	107697.0
49	100906.5
50	94116.0
51	85552.0
52	76988.0
53	68564.0
54	60140.0
55	52648.0
56	45156.0
57	40487.0
58	35818.0
59	31398.5
60	26979.0
61	23688.0
62	20397.0
63	18034.5
64	15672.0
65	13222.0
66	10772.0
67	8653.5
68	6535.0
69	5743.0
70	4951.0
71	4177.5
72	3404.0
73	2782.5
74	2161.0
75	1815.0
76	1469.0
77	1228.5
78	988.0
79	748.0
80	508.0
81	371.0
82	234.0
83	164.0
84	94.0
85	68.5
86	43.0
87	37.0
88	31.0
89	26.5
90	22.0
91	20.5
92	19.0
93	10.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1241170.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.5368099166758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.09591582158032	22.286083050691516
2	7.20867816905886	4.114253573218464
3	3.0015247591582033	2.5696182453688126
4	1.8091449768677195	2.065089052663318
5	1.271063624588107	1.813605052343589
6	0.9471257293209674	1.6216768142895226
7	0.7302642562486605	1.4587588588657454
8	0.6118277977465345	1.3967690852824977
9	0.5079724442031038	1.304632177481986
>10	4.587815776828264	28.394213468845276
>50	0.8955370931544993	18.012351487420375
>100	0.33199452722014683	12.99161489840455
>500	5.675120122990984E-4	0.10607713485368256
>1k	2.837560061495492E-4	0.09627917048933479
>5k	0.0	0.0
>10k+	2.837560061495492E-4	1.7689779297813353
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21846	1.7601134413496942	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04294335183737925	0.0
2	0.0	0.0	0.0	0.152517382792043	0.0
3	0.0	0.0	0.0	0.20867407365630816	0.0
4	0.0	0.0	0.0	0.34137144790802226	0.0
5	0.0	0.0	0.0	0.5860599273266354	0.0
6	0.0	0.0	0.0	0.8993127452323211	0.0
7	0.0	0.0	0.0	1.1189442219840957	0.0
8	0.0	0.0	0.0	1.7278857851865579	0.0
9	0.0	0.0	0.0	2.0311480296816713	0.0
10	8.056914040784099E-5	0.0	0.0	2.510695553389141	0.0
11	8.056914040784099E-5	0.0	0.0	2.8251569084009445	0.0
12	8.056914040784099E-5	0.0	0.0	3.127855168913203	0.0
13	1.6113828081568197E-4	0.0	0.0	3.2462112361723214	0.0
14	1.6113828081568197E-4	0.0	0.0	3.290524263396634	0.0
15	1.6113828081568197E-4	0.0	0.0	3.3490174593327264	0.0
16	1.6113828081568197E-4	0.0	0.0	3.4786532062489424	0.0
17	1.6113828081568197E-4	0.0	0.0	3.641161162451558	0.0
18	2.4170742122352296E-4	0.0	0.0	3.8929397262260608	0.0
19	3.2227656163136395E-4	0.0	0.0	3.9965516407905444	0.0
20	3.2227656163136395E-4	0.0	0.0	4.119419579912502	0.0
21	3.2227656163136395E-4	0.0	0.0	4.2577567939927645	0.0
22	3.2227656163136395E-4	0.0	0.0	4.405923443202784	0.0
23	3.2227656163136395E-4	0.0	0.0	4.561019038487879	0.0
24	4.0284570203920496E-4	0.0	0.0	4.6818727490996395	0.0
25	4.834148424470459E-4	0.0	0.0	4.77404384572621	0.0
26	4.834148424470459E-4	0.0	0.0	4.874755271236011	0.0
27	4.834148424470459E-4	0.0	0.0	4.979172877204573	0.0
28	6.445531232627279E-4	0.0	0.0	5.085685280823739	8.056914040784099E-5
29	6.445531232627279E-4	0.0	0.0	5.206941837137539	8.056914040784099E-5
30	6.445531232627279E-4	0.0	0.0	5.3721085749736135	8.056914040784099E-5
31	6.445531232627279E-4	0.0	0.0	5.507948145701233	8.056914040784099E-5
32	6.445531232627279E-4	0.0	0.0	5.619214128604462	8.056914040784099E-5
33	7.251222636705689E-4	0.0	0.0	5.741276376322341	8.056914040784099E-5
34	8.056914040784099E-4	0.0	0.0	5.873732043152832	8.056914040784099E-5
35	8.862605444862508E-4	0.0	0.0	6.030841866948122	8.056914040784099E-5
36	8.862605444862508E-4	0.0	0.0	6.167728836501043	8.056914040784099E-5
37	8.862605444862508E-4	0.0	0.0	6.303084992386216	8.056914040784099E-5
38	8.862605444862508E-4	0.0	0.0	6.4572943271268235	8.056914040784099E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACACG	20	7.856724E-4	44.000004	1
CGTGCAT	20	7.856724E-4	44.000004	37
ATACCGG	40	8.312782E-9	44.000004	2
ATCGAGT	20	7.856724E-4	44.000004	12
ATCGTAG	30	2.5278805E-6	44.0	1
ATCCGGG	30	2.5278805E-6	44.0	3
ACGATGC	25	4.4430453E-5	44.0	28
ACGCTAG	25	4.4430453E-5	44.0	1
CGTTTTT	17570	0.0	43.323845	1
TATGGGC	235	0.0	41.19149	4
ACCCGCT	220	0.0	41.0	34
ATTAACG	70	0.0	40.857143	1
TACGCGG	70	0.0	40.857143	2
CGATGCG	55	7.8216544E-11	40.0	10
GTTTTTT	19400	0.0	39.702065	2
CTACGGG	190	0.0	39.36842	3
AGGGCGA	825	0.0	39.2	6
TGCGGAT	45	2.3512257E-8	39.11111	4
TAACGGG	310	0.0	39.032257	3
CGGTCTA	85	0.0	38.823532	31
>>END_MODULE