FastQCFastQC Report
Thu 26 May 2016
SRR1545979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545979_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1224998
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216181.7647375750817553No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12260.10008179605191193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG302.5278532E-644.012
ACGTTCA207.8566844E-444.040
CTATCGT302.5278532E-644.013
ATCGTAC302.5278532E-644.015
CGTTACA207.8566844E-444.037
GTTCGGA207.8566844E-444.031
AACGCTC207.8566844E-444.034
TGTTACG254.4430133E-544.01
CGTACAT502.7284841E-1144.035
TCGTACT302.5278532E-644.016
CCGAATC207.8566844E-444.028
ATCGACG254.4430133E-544.01
CGTTTTT171050.043.2154351
CGTTGAT1100.042.00000425
TACGCGG950.041.684212
CGACGGT1700.041.41176628
CGGTCTA1750.040.2285731
TCGTACA557.8216544E-1140.00000434
ATCGTAG501.3478711E-939.61
ACGTATG501.3478711E-939.61
Produced by FastQC (version 0.11.5)