##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545979_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1224998
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.99944652970862	31.0	28.0	33.0	25.0	33.0
2	28.678670495788566	31.0	28.0	33.0	16.0	33.0
3	28.072200117877742	31.0	26.0	33.0	16.0	34.0
4	31.97174934163158	35.0	32.0	35.0	25.0	37.0
5	33.60719772603711	35.0	33.0	35.0	28.0	37.0
6	33.492524069427056	35.0	33.0	37.0	28.0	37.0
7	34.28894985950998	35.0	33.0	37.0	31.0	37.0
8	34.78115147943099	35.0	35.0	37.0	32.0	37.0
9	36.70413421083136	39.0	37.0	39.0	32.0	39.0
10	36.4139933289687	39.0	35.0	39.0	32.0	39.0
11	36.39971330565437	39.0	35.0	39.0	32.0	39.0
12	35.254666538231085	37.0	34.0	39.0	30.0	39.0
13	35.246026524124936	37.0	34.0	39.0	27.0	39.0
14	35.988287327816046	38.0	34.0	40.0	29.0	41.0
15	36.58726789758024	38.0	35.0	40.0	31.0	41.0
16	36.21985586915244	38.0	34.0	40.0	31.0	41.0
17	35.8354903436577	37.0	34.0	40.0	30.0	41.0
18	35.46537300469062	37.0	34.0	39.0	29.0	40.0
19	34.86569773991468	37.0	33.0	39.0	27.0	40.0
20	35.326503390209616	35.0	34.0	39.0	30.0	40.0
21	35.41169291704966	35.0	34.0	39.0	30.0	40.0
22	35.744641215740764	36.0	34.0	40.0	31.0	41.0
23	36.10599527509433	36.0	35.0	40.0	32.0	41.0
24	35.68250233877933	35.0	34.0	40.0	31.0	41.0
25	35.33451564818881	35.0	34.0	39.0	30.0	41.0
26	35.45755503274291	35.0	34.0	40.0	30.0	41.0
27	35.79325598898937	36.0	35.0	40.0	31.0	41.0
28	35.7595408319034	36.0	35.0	40.0	31.0	41.0
29	35.92092313620104	36.0	35.0	40.0	31.0	41.0
30	35.569236031405765	36.0	35.0	40.0	30.0	41.0
31	34.82214011777978	35.0	34.0	39.0	29.0	41.0
32	34.74593917704355	35.0	34.0	40.0	27.0	41.0
33	34.36132140623903	36.0	34.0	40.0	24.0	41.0
34	33.87465612188755	36.0	34.0	40.0	21.0	41.0
35	33.50913634144709	36.0	33.0	40.0	18.0	41.0
36	33.085245037134754	35.0	33.0	40.0	15.0	41.0
37	32.96947995017135	35.0	33.0	40.0	15.0	41.0
38	32.838141776558004	35.0	33.0	40.0	15.0	41.0
39	32.61142956968093	35.0	32.0	40.0	12.0	41.0
40	32.46002442452967	35.0	32.0	39.0	12.0	40.0
41	32.185961936264384	35.0	31.0	39.0	10.0	40.0
42	32.36546998444079	35.0	32.0	39.0	10.0	40.0
43	32.15212841163822	35.0	32.0	39.0	10.0	40.0
44	31.974128937353367	35.0	31.0	39.0	10.0	40.0
45	31.919132929196618	35.0	31.0	39.0	10.0	40.0
46	31.739904065149496	35.0	31.0	39.0	10.0	40.0
47	31.740431412949246	35.0	31.0	39.0	10.0	40.0
48	31.74502325718083	35.0	31.0	39.0	10.0	40.0
49	31.81555806621725	35.0	31.0	39.0	10.0	40.0
50	31.866969578725843	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	1.0
12	9.0
13	18.0
14	50.0
15	110.0
16	322.0
17	655.0
18	1408.0
19	2489.0
20	3973.0
21	5687.0
22	8303.0
23	12130.0
24	17635.0
25	27004.0
26	38494.0
27	43146.0
28	39108.0
29	36550.0
30	38573.0
31	46505.0
32	61575.0
33	95235.0
34	155993.0
35	109275.0
36	108740.0
37	156571.0
38	176863.0
39	38572.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.750164490064474	22.18036274344938	27.599065467861987	24.47040729862416
2	38.64626717757906	20.102400167183944	29.149108814871532	12.102223840365452
3	26.084695648482693	18.71513259613485	42.481457112583044	12.718714642799418
4	23.57318134396954	24.587305448661954	37.97377628371638	13.86573692365212
5	19.788767002068575	29.17335375241429	38.464879126333265	12.573000119183869
6	17.97758037155979	35.91393618601826	35.73058894790032	10.377894494521623
7	75.08061237651	4.5884156545561705	17.92802927025187	2.402942698681957
8	76.51457390134514	3.2186174997836727	17.549987836714838	2.7168207621563463
9	73.13032347807915	4.867436518263704	18.951296247014284	3.0509437566428677
10	44.048398446364814	23.562079285027405	23.514242472232606	8.875279796375178
11	37.94757215930149	18.843051172328444	31.965848107507117	11.243528560862956
12	34.73491385292057	19.607623849181792	33.25278898414528	12.40467331375235
13	19.04933722340771	34.72805669886808	33.32862584265443	12.89398023506977
14	13.746716321169503	35.598915263535126	36.819080520947786	13.835287894347584
15	12.829000537143735	21.11334059320913	52.51780002906127	13.539858840585861
16	14.727697514608188	17.505089804228252	50.653552087431976	17.11366059373158
17	15.166228842822601	18.193499091427086	37.22381587561775	29.416456190132557
18	19.246317136844304	20.773013506960826	41.73459875036531	18.246070605829562
19	24.73791793945786	21.89007655522703	34.912056999276736	18.459948506038376
20	26.329185843568727	21.301014369003052	34.73573018078397	17.634069606644257
21	19.84876710002792	25.102653228821598	37.04748905712499	18.001090614025493
22	19.9843591581374	22.009586954427682	34.74217917090477	23.26387471653015
23	16.67578232780788	26.49457386869203	34.90895495339584	21.920688850104245
24	16.64696595423013	21.224687713775857	45.97240158759443	16.155944744399584
25	15.02933066013169	22.599955265233085	43.14709085239323	19.223623222241994
26	14.47398281466582	29.925436612957736	36.70716197087669	18.893418601499757
27	15.150718613418142	30.18029417190885	37.37018346152402	17.298803753148984
28	13.478960781976786	25.048122527546983	45.005379600619754	16.467537089856474
29	14.3379009598383	21.40142269620032	43.90186759488587	20.358808749075507
30	17.39635493282438	27.021431871725504	38.26438900308409	17.317824192366025
31	24.524284937608062	24.0436310916426	33.86944305215192	17.56264091859742
32	26.122001831839725	23.02118044274358	34.17491293863337	16.681904786783324
33	24.519305337641367	24.924122325097674	32.43066519292277	18.125907144338193
34	18.290642107170786	25.644531664541493	33.7204632170828	22.344363011204916
35	18.70435706833807	24.746326116450803	35.783078829516455	20.766237985694673
36	27.179064782146583	23.82028378821843	30.847723833018502	18.152927596616482
37	19.02370452849719	29.873763059204993	32.899237386510016	18.203295025787796
38	19.74084855648744	29.467721579953597	29.994334684628054	20.797095178930906
39	19.48974610570793	28.831067479293843	31.865358147523505	19.813828267474722
40	22.667955376253676	25.001836737692635	29.93572234403648	22.39448554201721
41	17.193905622703056	23.23056853970374	32.16478720781585	27.410738629777352
42	21.15219779950661	23.408038217205252	29.046414769656764	26.393349213631367
43	21.04680987234265	24.614489166512925	29.917273334323813	24.421427626820616
44	19.345664237819165	28.23514813901737	30.568458070951955	21.850729552211515
45	17.07512991857946	33.786422508444915	27.323309915608025	21.815137657367604
46	20.573666242720396	29.6684566015618	29.46110932426012	20.296767831457686
47	19.938563165001085	25.805348253629802	30.806499275917187	23.449589305451926
48	20.969503623679387	23.031547806608664	33.83099400978614	22.16795455992581
49	20.64664595370768	23.19465011371447	33.742748967753414	22.415954964824433
50	19.126643472070974	29.58984422831711	30.721029748619998	20.56248255099192
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	463.0
1	776.0
2	1089.0
3	17221.5
4	33354.0
5	24542.5
6	15731.0
7	16159.5
8	16588.0
9	17664.5
10	18741.0
11	19132.5
12	19524.0
13	18444.0
14	17364.0
15	16276.0
16	15188.0
17	14120.0
18	13052.0
19	11818.5
20	10585.0
21	10158.5
22	9732.0
23	9459.0
24	9186.0
25	9096.0
26	9006.0
27	9643.0
28	10280.0
29	12712.5
30	15145.0
31	17812.0
32	20479.0
33	23093.0
34	25707.0
35	30051.5
36	34396.0
37	36976.0
38	39556.0
39	47410.5
40	55265.0
41	72820.5
42	90376.0
43	103789.5
44	117203.0
45	118842.5
46	120482.0
47	113012.5
48	105543.0
49	99086.5
50	92630.0
51	84193.0
52	75756.0
53	67086.0
54	58416.0
55	51511.5
56	44607.0
57	40308.0
58	36009.0
59	31364.0
60	26719.0
61	23380.5
62	20042.0
63	17853.5
64	15665.0
65	13146.5
66	10628.0
67	8595.5
68	6563.0
69	5708.0
70	4853.0
71	4046.5
72	3240.0
73	2823.5
74	2407.0
75	1962.0
76	1517.0
77	1264.0
78	1011.0
79	738.5
80	466.0
81	338.0
82	210.0
83	159.0
84	108.0
85	77.0
86	46.0
87	33.0
88	20.0
89	22.0
90	24.0
91	23.5
92	23.0
93	12.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1224998.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.056765216973563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85609932295023	21.843902994133888
2	7.229315693224406	4.0566242616835915
3	2.926595812723039	2.463324348076447
4	1.8494370598924261	2.075568854918867
5	1.3219857147425118	1.854532140936182
6	0.9506359811145729	1.6003062317363328
7	0.7591460170973359	1.4909427096970385
8	0.6510320857859999	1.4612683503691513
9	0.5180136640928243	1.3080408977372922
>10	4.667377748948532	28.527148865405326
>50	0.9270682149475444	18.180984712314153
>100	0.34182812524658934	13.111332915572488
>500	8.787355404519036E-4	0.14866649478000468
>1k	2.9291184681730115E-4	0.10075462830308773
>5k	0.0	0.0
>10k+	2.9291184681730115E-4	1.7766015943361753
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21618	1.7647375750817553	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1226	0.10008179605191193	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.163278633924301E-5	0.0	0.0	0.04122455710131771	0.0
2	8.163278633924301E-5	0.0	0.0	0.15371453667679458	0.0
3	8.163278633924301E-5	0.0	0.0	0.2135513690634597	0.0
4	8.163278633924301E-5	0.0	0.0	0.3413883124707142	0.0
5	8.163278633924301E-5	0.0	0.0	0.5826131961031773	0.0
6	8.163278633924301E-5	0.0	0.0	0.8844912399856979	0.0
7	8.163278633924301E-5	0.0	0.0	1.0960017893906766	0.0
8	8.163278633924301E-5	0.0	0.0	1.701227267309824	0.0
9	8.163278633924301E-5	0.0	0.0	1.9977991800802941	0.0
10	8.163278633924301E-5	0.0	0.0	2.4658815769495135	0.0
11	8.163278633924301E-5	0.0	0.0	2.769800440490515	0.0
12	8.163278633924301E-5	0.0	0.0	3.0483315074800124	0.0
13	1.6326557267848602E-4	0.0	0.0	3.1626990411412916	0.0
14	1.6326557267848602E-4	0.0	0.0	3.204168496601627	0.0
15	1.6326557267848602E-4	0.0	0.0	3.256331847072403	0.0
16	1.6326557267848602E-4	0.0	0.0	3.3826994003255515	0.0
17	2.44898359017729E-4	0.0	0.0	3.541720068114397	0.0
18	2.44898359017729E-4	0.0	0.0	3.7858837320550727	0.0
19	3.2653114535697203E-4	0.0	0.0	3.8881696133381443	0.0
20	4.89796718035458E-4	0.0	0.0	4.006455520743708	0.0
21	5.71429504374701E-4	0.0	0.0	4.140741454271762	0.0
22	6.530622907139441E-4	0.0	0.0	4.281068213988921	0.0
23	6.530622907139441E-4	0.0	0.0	4.434864383452054	0.0
24	6.530622907139441E-4	0.0	0.0	4.547191097454853	0.0
25	6.530622907139441E-4	0.0	0.0	4.638864716513823	0.0
26	6.530622907139441E-4	0.0	0.0	4.73184446015422	0.0
27	6.530622907139441E-4	0.0	0.0	4.832579318496847	0.0
28	7.34695077053187E-4	0.0	0.0	4.938865206310541	0.0
29	7.34695077053187E-4	0.0	0.0	5.057477644861461	0.0
30	7.34695077053187E-4	0.0	0.0	5.215600352000575	0.0
31	7.34695077053187E-4	0.0	0.0	5.348988324878897	0.0
32	7.34695077053187E-4	0.0	0.0	5.460661976590982	0.0
33	8.163278633924301E-4	0.0	0.0	5.576743798765386	0.0
34	8.163278633924301E-4	0.0	0.0	5.697886853692823	0.0
35	8.163278633924301E-4	0.0	0.0	5.855927928045597	0.0
36	8.163278633924301E-4	0.0	0.0	5.981968950153388	0.0
37	8.163278633924301E-4	0.0	0.0	6.112826306655195	0.0
38	8.163278633924301E-4	0.0	0.0	6.258295931911726	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	30	2.5278532E-6	44.0	12
ACGTTCA	20	7.8566844E-4	44.0	40
CTATCGT	30	2.5278532E-6	44.0	13
ATCGTAC	30	2.5278532E-6	44.0	15
CGTTACA	20	7.8566844E-4	44.0	37
GTTCGGA	20	7.8566844E-4	44.0	31
AACGCTC	20	7.8566844E-4	44.0	34
TGTTACG	25	4.4430133E-5	44.0	1
CGTACAT	50	2.7284841E-11	44.0	35
TCGTACT	30	2.5278532E-6	44.0	16
CCGAATC	20	7.8566844E-4	44.0	28
ATCGACG	25	4.4430133E-5	44.0	1
CGTTTTT	17105	0.0	43.215435	1
CGTTGAT	110	0.0	42.000004	25
TACGCGG	95	0.0	41.68421	2
CGACGGT	170	0.0	41.411766	28
CGGTCTA	175	0.0	40.22857	31
TCGTACA	55	7.8216544E-11	40.000004	34
ATCGTAG	50	1.3478711E-9	39.6	1
ACGTATG	50	1.3478711E-9	39.6	1
>>END_MODULE