FastQCFastQC Report
Thu 26 May 2016
SRR1545978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545978_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1072059
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110731.0328722579634142No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT20510.19131409745172606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCACG454.802132E-1044.0000041
GATCGAA302.5275567E-644.09
CTATCGT207.8562455E-444.013
AGGCGTA302.5275567E-644.06
TCGTCGG207.8562455E-444.02
GCGATAA800.044.09
TCGTAAT302.5275567E-644.033
CGTAATC302.5275567E-644.034
GCGTACA207.8562455E-444.08
GCGTAAG351.4459692E-744.01
CGTTTTT160600.043.397261
GCGGAAT5800.042.482768
AGGCGAT1350.040.740746
CGGAATT5900.040.644079
GTAGGGT3000.040.3333364
ACGGGAT3950.040.1012655
TACGGGA3150.039.809524
GTTTTTT177450.039.660752
TACGAAG1000.039.6000021
TAACGGG2900.039.4482773
Produced by FastQC (version 0.11.5)