##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545978_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1072059
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.07790429444648	28.0	25.0	30.0	16.0	33.0
2	26.15213435081465	30.0	25.0	31.0	10.0	33.0
3	26.357176237501854	30.0	25.0	31.0	16.0	33.0
4	27.81939613398143	32.0	19.0	35.0	10.0	35.0
5	31.17312666560329	33.0	30.0	35.0	19.0	37.0
6	29.823029329542496	33.0	28.0	35.0	17.0	36.0
7	29.883763860011435	32.0	28.0	35.0	17.0	35.0
8	32.45678456129747	33.0	32.0	35.0	28.0	35.0
9	27.132679264853895	31.0	23.0	34.0	10.0	35.0
10	31.93036670556378	34.0	31.0	37.0	18.0	38.0
11	33.84036326358904	35.0	32.0	37.0	27.0	39.0
12	33.240335653168344	35.0	31.0	39.0	25.0	39.0
13	33.12326280549858	35.0	31.0	38.0	22.0	39.0
14	32.87684912863938	35.0	31.0	39.0	21.0	40.0
15	31.92327101400203	34.0	27.0	38.0	18.0	40.0
16	30.56146909824926	34.0	27.0	38.0	17.0	40.0
17	30.733360757197133	33.0	27.0	38.0	17.0	40.0
18	31.66055226438097	34.0	29.0	38.0	18.0	39.0
19	31.29381964985136	34.0	27.0	37.0	18.0	39.0
20	32.02694254700534	34.0	30.0	37.0	23.0	39.0
21	32.71002342221837	34.0	31.0	37.0	23.0	39.0
22	33.025416511591246	34.0	31.0	37.0	25.0	39.0
23	34.31557125120912	35.0	33.0	38.0	29.0	40.0
24	33.94645817067904	35.0	33.0	38.0	27.0	40.0
25	32.69674803345711	35.0	31.0	37.0	23.0	39.0
26	26.32747637956493	33.0	20.0	36.0	0.0	39.0
27	29.605816470921845	34.0	24.0	37.0	15.0	39.0
28	31.171299340801205	34.0	27.0	36.0	19.0	39.0
29	32.528414014527186	34.0	30.0	37.0	23.0	39.0
30	32.21629966261185	34.0	30.0	37.0	22.0	39.0
31	31.095371616674083	34.0	29.0	36.0	19.0	38.0
32	31.320126037839334	34.0	30.0	37.0	18.0	39.0
33	31.004618215975054	34.0	29.0	37.0	18.0	39.0
34	30.931756554443364	34.0	29.0	37.0	15.0	39.0
35	30.056417603881876	34.0	27.0	37.0	10.0	39.0
36	29.72320086860891	34.0	26.0	37.0	10.0	39.0
37	29.7146537643917	34.0	26.0	37.0	10.0	39.0
38	29.476943899542842	33.0	26.0	36.0	10.0	39.0
39	29.346310231060045	33.0	25.0	36.0	9.0	39.0
40	29.29446233836011	33.0	25.0	36.0	10.0	38.0
41	28.790372544794643	33.0	24.0	36.0	9.0	38.0
42	28.958260692741725	33.0	24.0	36.0	8.0	38.0
43	28.50913056091129	33.0	24.0	36.0	8.0	38.0
44	28.239930824702746	32.0	23.0	35.0	8.0	38.0
45	27.715291788978032	31.0	23.0	35.0	8.0	38.0
46	27.311404502923814	31.0	23.0	35.0	8.0	38.0
47	27.47512123866317	31.0	23.0	35.0	8.0	38.0
48	27.55302180197172	31.0	23.0	35.0	8.0	37.0
49	27.402415352140135	31.0	23.0	35.0	8.0	38.0
50	29.140011883674312	34.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	10.0
12	49.0
13	145.0
14	305.0
15	830.0
16	1599.0
17	2925.0
18	5049.0
19	8034.0
20	11867.0
21	16898.0
22	22521.0
23	29526.0
24	38154.0
25	47417.0
26	55199.0
27	59507.0
28	64612.0
29	74999.0
30	89833.0
31	104944.0
32	114143.0
33	111820.0
34	95911.0
35	72235.0
36	35908.0
37	7411.0
38	201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.806712130582365	23.00461075369919	27.43543032612944	25.753246789589006
2	38.5011459257373	17.36154446723548	30.89046405095242	13.246845556074804
3	27.16268414331674	14.367865947676387	44.41723823035859	14.052211678648282
4	24.88864885234861	24.414980891909867	36.94320928232494	13.753160973416575
5	18.890937905469755	29.584192661038244	39.30893728796643	12.215932145525572
6	17.967294710459033	34.14401632745959	37.12855355908584	10.760135402995544
7	77.10331241097738	3.2286469308125767	18.016172617365275	1.6518680408447668
8	79.16579218121392	1.9261999572784707	17.27125092928654	1.6367569322210813
9	67.41522621422888	3.0971243187175332	17.285709088772165	12.201940378281419
10	45.31159199260488	23.274465304614765	23.630229306409444	7.783713396370909
11	39.29895649399893	18.237335818271198	32.16222241499768	10.301485272732192
12	35.5323727518728	19.510679915937462	33.62286963683901	11.334077695350722
13	18.465121789006016	35.82536035796537	33.465882008359614	12.243635844668997
14	13.035476592239792	35.99876499334459	37.51230109536882	13.453457319046805
15	11.314302664312319	19.56067716422324	52.987009110506044	16.138011060958398
16	13.064672746555928	15.612200447923108	50.88880369457278	20.434323110948185
17	14.302384476973748	17.093368928389204	38.14211717825231	30.46212941638473
18	18.735256175266475	20.20019420572935	43.029721311980026	18.03482830702415
19	25.52508770506101	20.81891015326582	35.702419363113414	17.953582778559763
20	26.902903664816957	20.797456110158116	35.39599966046645	16.90364056455848
21	19.576907614226453	24.79051992474295	37.81918719025725	17.813385270773345
22	19.771673014265073	21.60058354997253	34.98249629917756	23.645247136584832
23	15.957237428163934	26.436418144896876	35.777788349335246	21.82855607760394
24	15.704825947079403	20.511464387687617	48.32028834233937	15.463421322893609
25	14.256957872654397	21.369439555099113	45.24825592621301	19.125346646033474
26	11.358889762597022	41.68324691085099	30.61818426038119	16.3396790661708
27	14.169555966602584	31.267215703613328	38.155829110151586	16.407399219632502
28	12.630368291297401	24.80432513509051	46.72196213081556	15.843344442796525
29	13.3957179595526	21.165905980920826	45.33677717364436	20.101598885882215
30	16.911662511111793	26.990212292420473	39.19233922759848	16.90578596886925
31	25.12884085670658	23.780314329715065	34.505563593048514	16.58528122052984
32	26.594152000962634	22.0572748328217	34.99443594055924	16.354137225656423
33	24.82503295061186	24.478783350543207	32.88345137720965	17.812732321635284
34	17.690444275921383	25.082481467904284	33.97042513518379	23.25664912099054
35	18.515398872636673	24.191579008244883	36.041579801111695	21.251442318006752
36	27.458096989064966	23.341998901179878	30.743084102647334	18.45682000710782
37	18.309533337251025	30.543561501745707	32.45707559005614	18.689829570947122
38	18.94158810289359	30.36157524912342	29.761421712797524	20.93541493518547
39	19.082998230507837	29.09466736438946	31.607868596784318	20.214465808318387
40	22.69968350622494	24.492495282442476	29.781756414525695	23.026064796806892
41	16.509912234308	22.495683539805178	32.149816381374535	28.844587844512287
42	20.350185950586674	22.65817459673395	28.353383535794208	28.638255916885168
43	20.49653983596052	23.819957670240164	29.96215693352698	25.721345560272336
44	18.366806304503765	27.951913094335296	30.741405090578038	22.939875510582905
45	16.27755561960676	35.023165702633904	26.508708942325004	22.190569735434337
46	19.674476871142353	29.639413502428503	28.87966054107097	21.806449085358175
47	19.267129887440895	25.328923128297976	30.146381868908335	25.257565115352794
48	20.135925354854535	22.50752990273856	34.69277343877529	22.66377130363161
49	20.203179116074768	23.13156272182781	33.24257340314292	23.4226847589545
50	18.59393932610052	30.450096496554764	30.292829032730474	20.66313514461424
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	292.0
1	468.5
2	645.0
3	9876.5
4	19108.0
5	15815.0
6	12522.0
7	12817.0
8	13112.0
9	14475.5
10	15839.0
11	16737.5
12	17636.0
13	17565.5
14	17495.0
15	16947.5
16	16400.0
17	15810.5
18	15221.0
19	14127.5
20	13034.0
21	12397.0
22	11760.0
23	11249.5
24	10739.0
25	10286.5
26	9834.0
27	10163.0
28	10492.0
29	12440.0
30	14388.0
31	16766.0
32	19144.0
33	21249.5
34	23355.0
35	26474.5
36	29594.0
37	31723.0
38	33852.0
39	40362.0
40	46872.0
41	62025.5
42	77179.0
43	88794.5
44	100410.0
45	101618.5
46	102827.0
47	96505.0
48	90183.0
49	84112.5
50	78042.0
51	71526.0
52	65010.0
53	58170.5
54	51331.0
55	45605.0
56	39879.0
57	35831.5
58	31784.0
59	27756.5
60	23729.0
61	20866.0
62	18003.0
63	15790.5
64	13578.0
65	11582.0
66	9586.0
67	7844.5
68	6103.0
69	5307.0
70	4511.0
71	3838.0
72	3165.0
73	2671.0
74	2177.0
75	1787.0
76	1397.0
77	1137.5
78	878.0
79	695.0
80	512.0
81	368.0
82	224.0
83	158.0
84	92.0
85	69.5
86	47.0
87	42.0
88	37.0
89	28.5
90	20.0
91	17.0
92	14.0
93	9.0
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1072059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.17289369728133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89682235106682	35.788956992050224
2	6.780843708939345	5.854972892478344
3	2.6156715144933003	3.3877832472667837
4	1.440189260457701	2.4870855138282617
5	0.9381280327132452	2.02508509153843
6	0.6894930174430829	1.7860445248252779
7	0.5240751742091012	1.5838089249859602
8	0.39964252556886365	1.3802979418638068
9	0.33068162295465	1.2848834299919014
>10	2.9008487617979712	26.248817870845574
>50	0.39357246770856824	11.566008716470034
>100	0.08933001800406504	5.228567779998229
>500	2.338482144261689E-4	0.05270061941619861
>1k	2.338482144261689E-4	0.207066993146788
>5k	0.0	0.0
>10k+	2.338482144261689E-4	1.1179194612941898
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11073	1.0328722579634142	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	2051	0.19131409745172606	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03040877414396036	0.0
2	0.0	0.0	0.0	0.12378050088661165	0.0
3	0.0	0.0	0.0	0.17144578796502805	0.0
4	0.0	0.0	0.0	0.25940736470660664	0.0
5	9.327844829435693E-5	0.0	0.0	0.42525644577397326	0.0
6	9.327844829435693E-5	0.0	0.0	0.608362039775796	0.0
7	9.327844829435693E-5	0.0	0.0	0.7344745018697665	0.0
8	9.327844829435693E-5	0.0	0.0	1.0997529053904682	0.0
9	9.327844829435693E-5	0.0	0.0	1.3248338011247516	0.0
10	9.327844829435693E-5	0.0	0.0	1.6362905399796093	0.0
11	9.327844829435693E-5	0.0	0.0	1.8503645788151586	0.0
12	9.327844829435693E-5	0.0	0.0	2.0493275090270218	0.0
13	9.327844829435693E-5	0.0	0.0	2.131412543526056	0.0
14	9.327844829435693E-5	0.0	0.0	2.159955748704129	0.0
15	9.327844829435693E-5	0.0	0.0	2.196334343538928	0.0
16	9.327844829435693E-5	0.0	0.0	2.268345305622172	0.0
17	9.327844829435693E-5	0.0	0.0	2.3874618840940656	0.0
18	9.327844829435693E-5	0.0	0.0	2.548180650505243	0.0
19	9.327844829435693E-5	0.0	0.0	2.6052670608613893	0.0
20	9.327844829435693E-5	0.0	0.0	2.683154565187177	0.0
21	9.327844829435693E-5	0.0	0.0	2.7637471445135016	0.0
22	9.327844829435693E-5	0.0	0.0	2.8462985712540076	0.0
23	9.327844829435693E-5	0.0	0.0	2.938644235065421	0.0
24	9.327844829435693E-5	0.0	0.0	3.0015139092158174	0.0
25	9.327844829435693E-5	0.0	0.0	3.054682624743601	0.0
26	9.327844829435693E-5	0.0	0.0	3.1105564152719207	0.0
27	9.327844829435693E-5	0.0	0.0	3.1840598325278737	0.0
28	9.327844829435693E-5	0.0	0.0	3.2559775161628233	0.0
29	9.327844829435693E-5	0.0	0.0	3.3444054851458733	0.0
30	9.327844829435693E-5	0.0	0.0	3.4541942187883317	0.0
31	9.327844829435693E-5	0.0	0.0	3.548312173117338	0.0
32	9.327844829435693E-5	0.0	0.0	3.63207621968567	0.0
33	9.327844829435693E-5	0.0	0.0	3.725727781773205	0.0
34	9.327844829435693E-5	0.0	0.0	3.8218045835163923	0.0
35	9.327844829435693E-5	0.0	0.0	3.9446522999200604	0.0
36	9.327844829435693E-5	0.0	0.0	4.046419087009204	0.0
37	9.327844829435693E-5	0.0	0.0	4.146227026684166	0.0
38	9.327844829435693E-5	0.0	0.0	4.2560157603266235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCACG	45	4.802132E-10	44.000004	1
GATCGAA	30	2.5275567E-6	44.0	9
CTATCGT	20	7.8562455E-4	44.0	13
AGGCGTA	30	2.5275567E-6	44.0	6
TCGTCGG	20	7.8562455E-4	44.0	2
GCGATAA	80	0.0	44.0	9
TCGTAAT	30	2.5275567E-6	44.0	33
CGTAATC	30	2.5275567E-6	44.0	34
GCGTACA	20	7.8562455E-4	44.0	8
GCGTAAG	35	1.4459692E-7	44.0	1
CGTTTTT	16060	0.0	43.39726	1
GCGGAAT	580	0.0	42.48276	8
AGGCGAT	135	0.0	40.74074	6
CGGAATT	590	0.0	40.64407	9
GTAGGGT	300	0.0	40.333336	4
ACGGGAT	395	0.0	40.101265	5
TACGGGA	315	0.0	39.80952	4
GTTTTTT	17745	0.0	39.66075	2
TACGAAG	100	0.0	39.600002	1
TAACGGG	290	0.0	39.448277	3
>>END_MODULE