FastQCFastQC Report
Thu 26 May 2016
SRR1545977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545977_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1101227
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269902.4509024933097354No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12290.11160278489357779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG408.312782E-944.01
ATCGCTA207.8563386E-444.025
CGTTTTT169650.043.4034771
CGGTCTA1200.042.16666431
TATACGG900.041.5555572
CGGGTAT603.6379788E-1240.3333326
TGTAACG603.6379788E-1240.3333321
TCGCCGT557.8216544E-1140.025
GTTTTTT191450.039.4149862
TCTAGCG452.3508619E-839.111111
GTTAGCG750.038.133331
TCACGAC1400.037.71428725
CGACGGT1400.037.71428728
CGTAAGG2100.037.7142872
CGCATGG1350.037.481482
GGGTACC6250.036.9600037
ACGTAGG1250.036.962
GCCAGTA5900.036.91525325
GGGCGAT14700.036.8163267
AGGGTAC3600.036.6666686
Produced by FastQC (version 0.11.5)