##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545977_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1101227
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.073850350563507	31.0	28.0	33.0	25.0	33.0
2	28.86509139350924	31.0	28.0	33.0	16.0	33.0
3	28.255984460969447	31.0	27.0	33.0	16.0	34.0
4	32.11262528070961	35.0	32.0	35.0	25.0	37.0
5	33.65745118853788	35.0	33.0	35.0	28.0	37.0
6	33.5245349051558	35.0	33.0	37.0	28.0	37.0
7	34.249024043180924	35.0	33.0	37.0	30.0	37.0
8	34.679462090922215	35.0	35.0	37.0	32.0	37.0
9	36.483843930452124	39.0	37.0	39.0	32.0	39.0
10	36.240388221502016	38.0	35.0	39.0	31.0	39.0
11	36.36586280576121	38.0	35.0	39.0	32.0	39.0
12	35.25574654453623	37.0	34.0	39.0	29.0	39.0
13	35.596409278014434	37.0	35.0	39.0	30.0	39.0
14	36.31121558043891	38.0	35.0	40.0	30.0	41.0
15	36.822419900710756	38.0	35.0	40.0	31.0	41.0
16	36.42872995304329	38.0	35.0	40.0	31.0	41.0
17	36.0016999219961	38.0	34.0	40.0	30.0	41.0
18	35.583472798977866	38.0	34.0	39.0	29.0	40.0
19	34.98598290815608	37.0	33.0	39.0	27.0	40.0
20	35.36386866649655	36.0	34.0	39.0	30.0	40.0
21	35.56391915563276	36.0	34.0	39.0	30.0	40.0
22	35.87175486979524	36.0	34.0	40.0	31.0	41.0
23	36.16592673445166	37.0	35.0	40.0	31.0	41.0
24	35.83127275302912	36.0	35.0	40.0	31.0	41.0
25	35.49642171868289	36.0	34.0	40.0	30.0	41.0
26	35.57324965697354	36.0	34.0	40.0	30.0	41.0
27	35.87456718732832	37.0	35.0	40.0	31.0	41.0
28	35.79377639669205	36.0	35.0	40.0	31.0	41.0
29	35.88626868029934	37.0	35.0	40.0	31.0	41.0
30	35.51470314476489	36.0	35.0	40.0	30.0	41.0
31	34.816846118012	36.0	34.0	40.0	27.0	41.0
32	34.689776040725484	36.0	34.0	40.0	26.0	41.0
33	34.27000336896934	36.0	33.0	40.0	23.0	41.0
34	33.80952337710572	36.0	33.0	40.0	20.0	41.0
35	33.44869677187356	36.0	33.0	40.0	17.0	41.0
36	33.001665415032505	36.0	33.0	40.0	15.0	41.0
37	32.89553743233684	36.0	33.0	40.0	15.0	41.0
38	32.698707895828925	35.0	32.0	40.0	12.0	41.0
39	32.48429796944681	35.0	32.0	40.0	11.0	41.0
40	32.29994451643485	35.0	31.0	39.0	10.0	40.0
41	31.990305359385484	35.0	31.0	39.0	10.0	40.0
42	32.11460852303839	35.0	31.0	39.0	10.0	40.0
43	31.912003610518084	35.0	31.0	39.0	10.0	40.0
44	31.658622609144164	35.0	31.0	39.0	10.0	40.0
45	31.64229990728524	35.0	30.0	39.0	10.0	40.0
46	31.47132335113469	35.0	30.0	39.0	10.0	40.0
47	31.432622883383715	35.0	30.0	38.0	10.0	40.0
48	31.42088325113714	35.0	30.0	38.0	10.0	40.0
49	31.39380981396206	35.0	30.0	38.0	10.0	40.0
50	31.532822932964773	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	6.0
12	4.0
13	27.0
14	75.0
15	157.0
16	330.0
17	794.0
18	1475.0
19	2396.0
20	3767.0
21	5472.0
22	7706.0
23	10951.0
24	16037.0
25	24440.0
26	34872.0
27	39492.0
28	36943.0
29	34694.0
30	36336.0
31	42685.0
32	54839.0
33	80463.0
34	121537.0
35	100984.0
36	107753.0
37	146449.0
38	157542.0
39	32996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.76450904309466	21.25946784813667	26.278596510982748	25.69742659778592
2	41.44713124541988	19.582338609569145	26.96637478013162	12.004155364879358
3	27.352580349010697	18.28124446639975	41.505429852337436	12.860745332252115
4	24.47006838735338	23.826059477292148	37.96319923140279	13.740672903951683
5	20.21508735256219	27.786278396733827	38.64380368443563	13.354830566268353
6	18.134771486714367	34.882453844665996	36.80467333256449	10.178101336055146
7	72.90685753255232	4.8668439840287245	19.72862997365666	2.4976685097622924
8	73.92899011738724	3.702960425053145	19.557094041464655	2.8109554160949557
9	69.96868039014663	5.343948159643743	20.78881102624618	3.8985604239634513
10	39.981493370576636	22.143118539592656	26.121771442218545	11.753616647612164
11	34.05410510276264	20.894420496409914	31.84565943261471	13.205814968212731
12	30.327716265583753	19.686767578346696	36.230495619885815	13.755020536183729
13	20.49205113932005	28.500572543172296	36.159756344513895	14.847619972993762
14	15.99633862954686	30.62184272634071	38.41315187513564	14.968666768976787
15	15.806096290773835	20.714893477911456	48.296854327037025	15.182155904277684
16	18.116791542524837	17.962872323326618	46.74567550559512	17.174660628553422
17	18.136587642693105	18.19579432760003	37.420895056151004	26.24672297355586
18	20.529282336884222	20.762476764554446	40.70677526068649	18.001465637874844
19	24.530183150249677	21.581199879770473	35.49213740672904	18.39647956325081
20	25.712591500208404	21.37197871101962	35.10393406627335	17.81149572249863
21	21.63532132793693	23.273857251956226	37.39628614263907	17.69453527746777
22	21.329571468916036	20.824044452233736	35.777364703190166	22.06901937566006
23	18.853061176306067	24.386888443527084	35.42784548508164	21.332204895085212
24	18.84134697024319	21.589100158277994	42.21654572581311	17.353007145665696
25	18.336001569158768	21.78379207919893	40.21959141938946	19.660614932252845
26	16.891794334864656	25.96694414503095	36.78033684244938	20.360924677655014
27	17.514645027773565	26.03913634518587	37.81209505397162	18.63412357306895
28	16.98260213380166	23.582785383939914	41.80037358328483	17.6342388989736
29	17.653217728951436	21.99165113096573	40.97138918678892	19.383741953293917
30	19.638730252709024	24.156690673221778	38.561350203000835	17.643228871068363
31	24.927648886196945	22.90308900889644	34.37828894496775	17.79097315993887
32	25.274534678136295	22.42062717314414	34.601131283559155	17.703706865160406
33	24.16786003249103	23.905607109160965	32.961505665952615	18.965027192395393
34	19.886272312611297	24.810506825568208	34.39172849921043	20.91149236261007
35	21.31758483945635	24.03873134240261	34.290750226792476	20.35293359134856
36	26.055572556793464	24.129902372535366	30.8311547028905	18.983370367780665
37	21.302601552631746	27.940833270524607	31.56560818069299	19.190956996150657
38	21.261647235311155	28.042083966339366	29.619233818277245	21.077034980072227
39	21.376609908765403	27.318890655605067	30.197043842913406	21.107455592716125
40	23.001161431748404	24.470159195152316	30.478093980623434	22.050585392475845
41	19.635097940751546	23.74233468667223	31.408419880733035	25.214147491843192
42	21.654572581311573	24.85890738240163	29.331373095646946	24.15514694063985
43	21.2470271796823	24.981225487569773	30.46456361858182	23.307183714166108
44	20.443650582486626	26.82762046335587	30.79119927135822	21.93752968279928
45	19.332526354693446	30.062375877089824	28.87515471378744	21.729943054429288
46	21.545330799190356	27.074436060866653	30.59387392426811	20.786359215674878
47	21.312772026112693	25.518262810483215	31.330597597044026	21.83836756636007
48	21.70342717713968	24.195011564373196	32.72894689287495	21.37261436561218
49	21.286619380018834	23.523851122429797	33.07546945361855	22.114060043932813
50	19.958736936163028	27.146265029825823	31.551169740662004	21.343828293349144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	495.0
1	1015.5
2	1536.0
3	21643.5
4	41751.0
5	29070.5
6	16390.0
7	16349.0
8	16308.0
9	16738.5
10	17169.0
11	17098.5
12	17028.0
13	16148.5
14	15269.0
15	14272.0
16	13275.0
17	12330.5
18	11386.0
19	10606.5
20	9827.0
21	9295.5
22	8764.0
23	8630.0
24	8496.0
25	8225.5
26	7955.0
27	8447.5
28	8940.0
29	10540.0
30	12140.0
31	13849.0
32	15558.0
33	17609.0
34	19660.0
35	22436.0
36	25212.0
37	27302.5
38	29393.0
39	35744.0
40	42095.0
41	53139.5
42	64184.0
43	72703.0
44	81222.0
45	82854.0
46	84486.0
47	82455.5
48	80425.0
49	79767.0
50	79109.0
51	77028.0
52	74947.0
53	67194.0
54	59441.0
55	54923.0
56	50405.0
57	47377.5
58	44350.0
59	40398.0
60	36446.0
61	33648.0
62	30850.0
63	27484.5
64	24119.0
65	20519.0
66	16919.0
67	14526.0
68	12133.0
69	10416.0
70	8699.0
71	7473.0
72	6247.0
73	4934.5
74	3622.0
75	2958.0
76	2294.0
77	1808.0
78	1322.0
79	970.5
80	619.0
81	477.0
82	335.0
83	260.0
84	185.0
85	151.0
86	117.0
87	76.0
88	35.0
89	30.5
90	26.0
91	23.5
92	21.0
93	18.0
94	15.0
95	9.5
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1101227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.4545330756072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3426175560276	24.776640065505287
2	7.251525905254723	4.706897746814238
3	3.3566022988291992	3.2681088098703426
4	2.1014362191956395	2.7280452512865527
5	1.561556888935557	2.5339799850700664
6	1.1822736802576301	2.3022084156204667
7	0.9418822547360085	2.139784415276009
8	0.7590805460457791	1.9708483750954169
9	0.6089707422299048	1.7787474986199763
>10	5.237588004283675	35.22858280028898
>50	0.5978082358013919	13.004689991321353
>100	0.05753503360135926	2.889080679913353
>500	5.613174007911319E-4	0.10201684723938208
>1k	2.8065870039556596E-4	0.11194527255107194
>5k	0.0	0.0
>10k+	2.8065870039556596E-4	2.458423845527609
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26990	2.4509024933097354	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1229	0.11160278489357779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024427297914054052	0.0
2	0.0	0.0	0.0	0.11296490187763286	0.0
3	0.0	0.0	0.0	0.16645069545152816	0.0
4	0.0	0.0	0.0	0.2519916420501858	0.0
5	0.0	0.0	0.0	0.45458384147864156	0.0
6	0.0	0.0	0.0	0.6848724195828835	0.0
7	0.0	0.0	0.0	0.8511414994365376	0.0
8	0.0	0.0	0.0	1.2378011073103001	0.0
9	0.0	0.0	0.0	1.4543777077750546	0.0
10	0.0	0.0	0.0	1.812886897978346	0.0
11	0.0	0.0	0.0	2.0826768686201844	0.0
12	0.0	0.0	0.0	2.3321258923001342	0.0
13	0.0	0.0	0.0	2.4366456688766256	0.0
14	9.08077989370039E-5	0.0	0.0	2.4801426045674506	0.0
15	9.08077989370039E-5	0.0	0.0	2.536261824310519	0.0
16	9.08077989370039E-5	0.0	0.0	2.648318648198782	0.0
17	9.08077989370039E-5	0.0	0.0	2.775721990107398	0.0
18	9.08077989370039E-5	0.0	0.0	2.97540833996987	0.0
19	9.08077989370039E-5	0.0	0.0	3.0568629356163624	0.0
20	1.816155978740078E-4	0.0	0.0	3.1543905116747046	0.0
21	1.816155978740078E-4	0.0	0.0	3.2575481712671412	0.0
22	1.816155978740078E-4	0.0	0.0	3.3657910676000498	0.0
23	1.816155978740078E-4	0.0	0.0	3.486202208990517	0.0
24	1.816155978740078E-4	0.0	0.0	3.579371010699883	0.0
25	1.816155978740078E-4	0.0	0.0	3.65855541137295	0.0
26	1.816155978740078E-4	0.0	0.0	3.7357420404694035	0.0
27	1.816155978740078E-4	0.0	0.0	3.8263682238085335	0.0
28	3.632311957480156E-4	0.0	0.0	3.906460702470971	0.0
29	3.632311957480156E-4	0.0	0.0	3.998449002794156	0.0
30	3.632311957480156E-4	0.0	0.0	4.121130339158048	0.0
31	3.632311957480156E-4	0.0	0.0	4.217023374835525	0.0
32	3.632311957480156E-4	0.0	0.0	4.306559864587411	0.0
33	3.632311957480156E-4	0.0	0.0	4.403451786053194	0.0
34	4.5403899468501955E-4	0.0	0.0	4.508879640619055	0.0
35	5.448467936220234E-4	0.0	0.0	4.63392197975531	0.0
36	6.356545925590274E-4	0.0	0.0	4.7325392494008955	0.0
37	6.356545925590274E-4	0.0	0.0	4.828250669480497	0.0
38	7.264623914960312E-4	0.0	0.0	4.945392730109233	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAG	40	8.312782E-9	44.0	1
ATCGCTA	20	7.8563386E-4	44.0	25
CGTTTTT	16965	0.0	43.403477	1
CGGTCTA	120	0.0	42.166664	31
TATACGG	90	0.0	41.555557	2
CGGGTAT	60	3.6379788E-12	40.333332	6
TGTAACG	60	3.6379788E-12	40.333332	1
TCGCCGT	55	7.8216544E-11	40.0	25
GTTTTTT	19145	0.0	39.414986	2
TCTAGCG	45	2.3508619E-8	39.11111	1
GTTAGCG	75	0.0	38.13333	1
TCACGAC	140	0.0	37.714287	25
CGACGGT	140	0.0	37.714287	28
CGTAAGG	210	0.0	37.714287	2
CGCATGG	135	0.0	37.48148	2
GGGTACC	625	0.0	36.960003	7
ACGTAGG	125	0.0	36.96	2
GCCAGTA	590	0.0	36.915253	25
GGGCGAT	1470	0.0	36.816326	7
AGGGTAC	360	0.0	36.666668	6
>>END_MODULE