FastQCFastQC Report
Thu 26 May 2016
SRR1545976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545976_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1107378
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274422.4781059403383487No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12840.11594956735640405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG207.856356E-444.0000049
ATCGCTC207.856356E-444.00000428
TACCGGC207.856356E-444.00000442
CGGGTAT750.044.06
TATAGCG454.802132E-1044.01
GCGTATA302.5276331E-644.025
CGTTTTT168200.043.4114151
CGGTCTA1550.042.58064731
TCACGAC1500.042.53333325
ACGTAGG1500.041.0666662
TCTAGCG603.6379788E-1240.3333321
GTACGAG603.6379788E-1240.3333321
ACGGTCT1600.039.87500430
GTTTTTT188300.039.6887972
ATAACGG501.3478711E-939.62
TCGTTGA1450.039.44827724
TATCGCG452.3508619E-839.111111
CGACAGT404.1243584E-738.50000444
ACGACGG1600.038.50000427
GCCCTAC2900.037.93103826
Produced by FastQC (version 0.11.5)