##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545976_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1107378
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.032523673036668	31.0	28.0	33.0	25.0	33.0
2	28.82844791931933	31.0	28.0	33.0	16.0	33.0
3	28.234526963692616	31.0	27.0	33.0	16.0	34.0
4	32.09841084074273	35.0	32.0	35.0	25.0	37.0
5	33.68500457838245	35.0	33.0	35.0	28.0	37.0
6	33.604388925913284	35.0	33.0	37.0	28.0	37.0
7	34.307856937739416	35.0	35.0	37.0	31.0	37.0
8	34.714144582969865	35.0	35.0	37.0	32.0	37.0
9	36.586358045762154	39.0	37.0	39.0	32.0	39.0
10	36.24062695845502	38.0	35.0	39.0	31.0	39.0
11	36.41493419591142	39.0	35.0	39.0	32.0	39.0
12	35.19025752724002	37.0	34.0	39.0	29.0	39.0
13	35.57546655252317	37.0	35.0	39.0	30.0	39.0
14	36.305203823807226	38.0	35.0	40.0	30.0	41.0
15	36.84215958778303	38.0	35.0	40.0	31.0	41.0
16	36.445561497519364	38.0	35.0	40.0	31.0	41.0
17	36.03744791751326	38.0	34.0	40.0	30.0	41.0
18	35.65270846991723	38.0	34.0	39.0	29.0	40.0
19	35.05290244162337	37.0	33.0	39.0	27.0	41.0
20	35.333597019265326	35.0	34.0	39.0	30.0	40.0
21	35.45036473543812	36.0	34.0	39.0	30.0	40.0
22	35.83537057806819	36.0	34.0	40.0	31.0	41.0
23	36.18352450563403	37.0	35.0	40.0	31.0	41.0
24	35.72423147290266	36.0	35.0	40.0	31.0	41.0
25	35.409890750945024	36.0	34.0	40.0	30.0	41.0
26	35.541592843636046	36.0	34.0	40.0	30.0	41.0
27	35.8937481149165	37.0	35.0	40.0	31.0	41.0
28	35.79944607893601	36.0	35.0	40.0	31.0	41.0
29	35.9038485503595	37.0	35.0	40.0	31.0	41.0
30	35.55455228476636	36.0	35.0	40.0	30.0	41.0
31	34.85676706598831	36.0	34.0	40.0	28.0	41.0
32	34.77418189633531	36.0	34.0	40.0	26.0	41.0
33	34.37742396905122	36.0	34.0	40.0	23.0	41.0
34	33.84752993106238	36.0	33.0	40.0	20.0	41.0
35	33.51380468096711	36.0	33.0	40.0	18.0	41.0
36	33.05514828721539	36.0	33.0	40.0	15.0	41.0
37	32.94098582417205	36.0	33.0	40.0	15.0	41.0
38	32.79164296202381	36.0	32.0	40.0	13.0	41.0
39	32.533982976002775	35.0	32.0	40.0	12.0	41.0
40	32.31682225942723	35.0	31.0	39.0	10.0	40.0
41	32.01098179664035	35.0	31.0	39.0	10.0	40.0
42	32.13040804494942	35.0	31.0	39.0	10.0	40.0
43	31.948921687084265	35.0	31.0	39.0	10.0	40.0
44	31.68767033479083	35.0	31.0	39.0	10.0	40.0
45	31.657769072529884	35.0	30.0	39.0	10.0	40.0
46	31.479460491358868	35.0	30.0	38.0	10.0	40.0
47	31.446545804594276	35.0	30.0	38.0	10.0	40.0
48	31.441059872961176	35.0	30.0	38.0	10.0	40.0
49	31.472474620229047	35.0	30.0	38.0	10.0	40.0
50	31.625006998513605	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	0.0
11	2.0
12	13.0
13	26.0
14	50.0
15	132.0
16	330.0
17	710.0
18	1374.0
19	2294.0
20	3636.0
21	5526.0
22	7724.0
23	10890.0
24	15896.0
25	23891.0
26	34349.0
27	39418.0
28	37289.0
29	34937.0
30	36109.0
31	43113.0
32	54722.0
33	81058.0
34	122719.0
35	102661.0
36	109590.0
37	151497.0
38	158054.0
39	29364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.921701532809934	21.220305984045197	26.185457901457315	25.672534581687557
2	41.57126112312146	19.481875204311446	26.940665247097193	12.006198425469893
3	27.35768635461423	18.335834737551224	41.30540790949432	13.001070998340222
4	24.424541574782957	23.816167559767308	37.89717693506643	13.862113930383302
5	20.17368956219105	27.696414413145288	38.75108589840145	13.378810126262216
6	18.108721683110915	34.911114362033565	36.8319580125305	10.148205942325024
7	72.77153781274326	4.947542754145378	19.73084168188279	2.550077751228578
8	73.84903799786522	3.730072296903135	19.573171943094408	2.8477177621372287
9	69.92264610638824	5.366640839893876	20.86866453911853	3.842048514599351
10	39.91238763999284	22.019310479348515	26.060839207569593	12.007462673089044
11	33.95588498236374	20.830827413945375	31.88838860804531	13.32489899564557
12	30.36180960792069	19.35987530906339	36.1474582301617	14.13085685285422
13	20.608951956784406	28.3865130063989	36.16569951723802	14.83883551957868
14	16.030117990424227	30.719230470534903	38.292886439860645	14.957765099180225
15	15.788375784962316	20.70828569828911	48.28540931822738	15.21792919852119
16	18.086597349775776	17.928024576973716	46.740498727625074	17.24487934562543
17	18.133916332092564	18.2134736286977	37.417936784006905	26.23467325520283
18	20.509347305075593	20.787391477887407	40.67825078699414	18.025010430042858
19	24.43050159927324	21.64256468884157	35.46620937024214	18.460724341643054
20	25.585391799367514	21.251460657517125	35.08828963551741	18.074857907597945
21	21.599851179994545	23.219984503936324	37.464172125507275	17.71599219056185
22	21.353322894260135	20.672706158150152	35.892712334902804	22.081258612686906
23	18.790241453234575	24.318886595182494	35.427198300851195	21.463673650731728
24	18.859233251879665	21.555150996317426	42.16338052589089	17.42223522591202
25	18.32129588993099	21.734945068440947	40.18844513797457	19.755313903653494
26	16.954192696622112	25.99166680212177	36.761340752660786	20.292799748595332
27	17.60076505041639	25.93495626606272	37.716931345936075	18.74734733758482
28	16.985257066692675	23.583094480836717	41.71249564286089	17.719152809609724
29	17.595708059939785	22.009467408599413	40.96071982647299	19.434104704987817
30	19.598818109082895	24.22596439517491	38.48288479633874	17.692332699403458
31	24.90360111903975	22.890557695746168	34.39936498648158	17.8064761987325
32	25.127282644228078	22.446445567818756	34.70639655113249	17.71987523682067
33	24.11019543462124	23.987292505359505	33.00255197412266	18.899960085896595
34	19.93447585196744	24.715860347595854	34.49635083955072	20.853312960885983
35	21.260581301055286	24.024045989716246	34.342022326612955	20.373350382615513
36	26.110054561315106	23.955325101275264	30.913292480074556	19.021327857335073
37	21.30374632690915	27.980779824052853	31.570972152237086	19.144501696800912
38	21.349439848001314	27.9192832077213	29.675232847320427	21.056044096956956
39	21.39693943712084	27.342154169578954	30.244144275938297	21.01676211736191
40	22.980319276705877	24.48025877342696	30.479113726297612	22.06030822356955
41	19.74167809004694	23.83720825228603	31.342504546776258	25.07860911089077
42	21.759326986810283	24.840388738082208	29.374974037772105	24.025310237335397
43	21.31620819629792	24.980810527209318	30.42773109091927	23.27525018557349
44	20.499684841129227	26.85821824164829	30.79553684469079	21.84656007253169
45	19.289709566200518	30.04078101605775	28.99010094114205	21.67940847659968
46	21.60265058543695	27.03187168247879	30.62883676576562	20.736640966318635
47	21.269160124185237	25.59324819528652	31.358939765825223	21.77865191470302
48	21.715529837146846	24.319247808787967	32.60819702034897	21.35702533371622
49	21.34320891330693	23.559796203283792	33.05212854147364	22.044866341935634
50	19.992721545849747	27.134546649834114	31.466762027058508	21.40596977725763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	465.0
1	966.0
2	1467.0
3	21994.0
4	42521.0
5	29399.0
6	16277.0
7	16308.5
8	16340.0
9	16843.5
10	17347.0
11	17086.5
12	16826.0
13	16037.5
14	15249.0
15	14284.0
16	13319.0
17	12362.0
18	11405.0
19	10486.0
20	9567.0
21	9020.0
22	8473.0
23	8636.0
24	8799.0
25	8366.0
26	7933.0
27	8450.5
28	8968.0
29	10463.5
30	11959.0
31	13954.0
32	15949.0
33	17910.0
34	19871.0
35	22581.5
36	25292.0
37	27585.0
38	29878.0
39	36194.5
40	42511.0
41	53333.5
42	64156.0
43	72880.0
44	81604.0
45	83216.5
46	84829.0
47	83006.0
48	81183.0
49	80114.5
50	79046.0
51	77296.5
52	75547.0
53	67951.0
54	60355.0
55	55423.5
56	50492.0
57	47517.5
58	44543.0
59	40746.5
60	36950.0
61	34079.0
62	31208.0
63	27701.0
64	24194.0
65	20414.5
66	16635.0
67	14306.0
68	11977.0
69	10374.0
70	8771.0
71	7625.5
72	6480.0
73	5160.5
74	3841.0
75	3118.5
76	2396.0
77	1870.5
78	1345.0
79	1014.5
80	684.0
81	529.0
82	374.0
83	260.0
84	146.0
85	124.0
86	102.0
87	74.0
88	46.0
89	39.0
90	32.0
91	24.5
92	17.0
93	10.5
94	4.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1107378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.02505597521998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76630861640521	24.264202744761683
2	7.40742780779098	4.7444658035381435
3	3.4355162052334562	3.3006779632913035
4	2.209348561686518	2.8301804542712996
5	1.5802034693927571	2.530305227976993
6	1.2093393896389903	2.323749698773622
7	0.9379556293835167	2.1026657073295865
8	0.788899978397344	2.021165277361982
9	0.643631417998037	1.855109896991761
>10	5.3522406903685305	35.30876486398557
>50	0.6060248431343278	12.963637481331592
>100	0.06196639254237602	3.0447159889957356
>500	5.684990140024469E-4	0.09540055478529721
>1k	2.8424950700122343E-4	0.11688388582473438
>5k	0.0	0.0
>10k+	2.8424950700122343E-4	2.4980744507806545
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27442	2.4781059403383487	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1284	0.11594956735640405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023659491158393973	0.0
2	0.0	0.0	0.0	0.10249436055258457	0.0
3	0.0	0.0	0.0	0.16083035783625826	0.0
4	0.0	0.0	0.0	0.243819183693373	0.0
5	0.0	0.0	0.0	0.44320909391373137	0.0
6	0.0	0.0	0.0	0.6754694422320111	0.0
7	0.0	0.0	0.0	0.8322361470067131	0.0
8	0.0	0.0	0.0	1.22234684091611	0.0
9	0.0	0.0	0.0	1.4309476980759956	0.0
10	0.0	0.0	0.0	1.7883685606902069	0.0
11	0.0	0.0	0.0	2.0566599661542853	0.0
12	0.0	0.0	0.0	2.3077937253584593	0.0
13	0.0	0.0	0.0	2.406043826046752	0.0
14	0.0	0.0	0.0	2.445506412444531	0.0
15	0.0	0.0	0.0	2.498966026054337	0.0
16	9.030340136791592E-5	0.0	0.0	2.6085040519136196	0.0
17	9.030340136791592E-5	0.0	0.0	2.729510609746627	0.0
18	9.030340136791592E-5	0.0	0.0	2.918425325408307	0.0
19	9.030340136791592E-5	0.0	0.0	2.999698386639431	0.0
20	9.030340136791592E-5	0.0	0.0	3.0946975648784787	0.0
21	9.030340136791592E-5	0.0	0.0	3.1933991825736108	0.0
22	9.030340136791592E-5	0.0	0.0	3.309168143127279	0.0
23	9.030340136791592E-5	0.0	0.0	3.420873450619391	0.0
24	9.030340136791592E-5	0.0	0.0	3.517227179878957	0.0
25	9.030340136791592E-5	0.0	0.0	3.591456575803384	0.0
26	9.030340136791592E-5	0.0	0.0	3.664963544516868	0.0
27	9.030340136791592E-5	0.0	0.0	3.7544542152724723	0.0
28	1.8060680273583184E-4	0.0	0.0	3.836810917320012	0.0
29	1.8060680273583184E-4	0.0	0.0	3.9188064057620795	0.0
30	1.8060680273583184E-4	0.0	0.0	4.041619031622445	0.0
31	1.8060680273583184E-4	0.0	0.0	4.140410952718945	0.0
32	1.8060680273583184E-4	0.0	0.0	4.232520422114219	0.0
33	1.8060680273583184E-4	0.0	0.0	4.328874151373785	0.0
34	1.8060680273583184E-4	0.0	0.0	4.431458815327738	0.0
35	1.8060680273583184E-4	0.0	0.0	4.5445186738403685	0.0
36	1.8060680273583184E-4	0.0	0.0	4.634551165004181	0.0
37	1.8060680273583184E-4	0.0	0.0	4.734426726917096	0.0
38	1.8060680273583184E-4	0.0	0.0	4.850556901076236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	20	7.856356E-4	44.000004	9
ATCGCTC	20	7.856356E-4	44.000004	28
TACCGGC	20	7.856356E-4	44.000004	42
CGGGTAT	75	0.0	44.0	6
TATAGCG	45	4.802132E-10	44.0	1
GCGTATA	30	2.5276331E-6	44.0	25
CGTTTTT	16820	0.0	43.411415	1
CGGTCTA	155	0.0	42.580647	31
TCACGAC	150	0.0	42.533333	25
ACGTAGG	150	0.0	41.066666	2
TCTAGCG	60	3.6379788E-12	40.333332	1
GTACGAG	60	3.6379788E-12	40.333332	1
ACGGTCT	160	0.0	39.875004	30
GTTTTTT	18830	0.0	39.688797	2
ATAACGG	50	1.3478711E-9	39.6	2
TCGTTGA	145	0.0	39.448277	24
TATCGCG	45	2.3508619E-8	39.11111	1
CGACAGT	40	4.1243584E-7	38.500004	44
ACGACGG	160	0.0	38.500004	27
GCCCTAC	290	0.0	37.931038	26
>>END_MODULE