FastQCFastQC Report
Thu 26 May 2016
SRR1545975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545975_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1090457
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271462.489414988394774No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12630.11582299898116111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC207.856302E-444.00000434
ATACGAG207.856302E-444.0000041
TACGTCA207.856302E-444.00000435
CGTTTTT161550.043.4280431
CGGTCTA1250.040.4831
TATAGCG501.3478711E-939.6000021
TATACGG1000.039.6000022
GCGATAT950.039.368429
GTTTTTT183500.038.9645772
TCACGAC1300.038.9230825
CGACGGT1300.038.9230828
ACGGTCT1300.038.9230830
TCGTTGA2100.038.76190624
TATGGGC2450.038.6122444
ACGTAGG1150.038.260872
TAGGGCG3800.038.2105265
TCTAGCG357.289864E-637.7142871
CGTAAGG1600.037.1250042
ACACGTG950.037.0526342
CGTTGAT2200.037.025
Produced by FastQC (version 0.11.5)