##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545975_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1090457
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.92749553627516	31.0	28.0	31.0	23.0	33.0
2	28.726783357803196	31.0	28.0	33.0	16.0	33.0
3	28.126299340551714	31.0	27.0	33.0	16.0	34.0
4	31.992537073905712	35.0	32.0	35.0	25.0	37.0
5	33.6255239775617	35.0	33.0	35.0	28.0	37.0
6	33.54975849574995	35.0	33.0	37.0	28.0	37.0
7	34.22259658106647	35.0	33.0	37.0	30.0	37.0
8	34.68016895668513	35.0	35.0	37.0	32.0	37.0
9	36.573372448432174	39.0	37.0	39.0	32.0	39.0
10	36.31486706949472	38.0	35.0	39.0	32.0	39.0
11	36.436666461859566	39.0	35.0	39.0	32.0	39.0
12	35.37243559351721	37.0	34.0	39.0	30.0	39.0
13	35.660274545442874	37.0	35.0	39.0	30.0	39.0
14	36.380792640149956	38.0	35.0	40.0	30.0	41.0
15	36.82752919188927	38.0	35.0	40.0	31.0	41.0
16	36.42331242772526	38.0	35.0	40.0	31.0	41.0
17	36.00526293104634	38.0	34.0	40.0	30.0	41.0
18	35.59821524370058	38.0	34.0	39.0	29.0	40.0
19	34.96353180363829	37.0	33.0	39.0	27.0	40.0
20	35.432083979469155	36.0	34.0	39.0	30.0	41.0
21	35.56099048380633	36.0	34.0	39.0	30.0	40.0
22	35.87082755211806	36.0	34.0	40.0	31.0	41.0
23	36.18576064897562	37.0	35.0	40.0	31.0	41.0
24	35.75592343393642	36.0	35.0	40.0	31.0	41.0
25	35.44605151784985	36.0	34.0	40.0	30.0	41.0
26	35.556261273942944	36.0	34.0	40.0	30.0	41.0
27	35.906957358245215	37.0	35.0	40.0	31.0	41.0
28	35.82407284285396	36.0	35.0	40.0	31.0	41.0
29	35.91304929951387	37.0	35.0	40.0	31.0	41.0
30	35.563763633045596	36.0	35.0	40.0	30.0	41.0
31	34.84443586496304	36.0	34.0	40.0	28.0	41.0
32	34.773135483563316	36.0	34.0	40.0	26.0	41.0
33	34.39066923317471	36.0	34.0	40.0	23.0	41.0
34	33.865106097718666	36.0	33.0	40.0	20.0	41.0
35	33.49612135095653	36.0	33.0	40.0	17.0	41.0
36	33.077766477724474	36.0	33.0	40.0	15.0	41.0
37	32.987220954150416	36.0	33.0	40.0	15.0	41.0
38	32.80687913416118	36.0	32.0	40.0	12.0	41.0
39	32.525593397997355	35.0	32.0	40.0	11.0	41.0
40	32.36395107739232	35.0	31.0	40.0	10.0	40.0
41	32.037733720816135	35.0	31.0	39.0	10.0	40.0
42	32.19868183706464	35.0	31.0	39.0	10.0	40.0
43	32.01120264256179	35.0	31.0	39.0	10.0	40.0
44	31.763724750265254	35.0	31.0	39.0	10.0	40.0
45	31.736924060279314	35.0	31.0	39.0	10.0	40.0
46	31.56616446132218	35.0	30.0	39.0	10.0	40.0
47	31.545880305229826	35.0	30.0	39.0	10.0	40.0
48	31.52523391568856	35.0	30.0	38.0	10.0	40.0
49	31.558281527836495	35.0	30.0	38.0	10.0	40.0
50	31.640973463419467	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	1.0
11	3.0
12	9.0
13	22.0
14	58.0
15	119.0
16	306.0
17	691.0
18	1273.0
19	2287.0
20	3646.0
21	5363.0
22	7678.0
23	10799.0
24	15561.0
25	23786.0
26	34113.0
27	39050.0
28	36637.0
29	34308.0
30	35769.0
31	41521.0
32	53901.0
33	79667.0
34	120037.0
35	99695.0
36	106188.0
37	146651.0
38	157760.0
39	33556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.88129839140837	21.279885405843604	26.09997459780624	25.738841604941783
2	41.611727926915044	19.42726765016869	26.962548729569345	11.998455693346918
3	27.360455295348647	18.391738509634035	41.31267899605395	12.93512719896337
4	24.39023271894261	23.882463957771833	37.788835323171845	13.938468000113712
5	20.10193891185072	27.71333486785815	38.77209280145847	13.412633418832653
6	18.171647300168644	34.849884039444014	36.821809571583294	10.156659088804052
7	72.68805647540435	5.004874103242953	19.758780034425932	2.5482893869267658
8	73.77191397735078	3.772363330236772	19.594628674032997	2.86109401837945
9	69.86822955879965	5.4101170426711	20.900044660174586	3.821608738354653
10	39.864387133101076	22.310921017518343	26.128678159707352	11.69601368967323
11	33.996388670071354	20.84217901301931	31.837110495874665	13.324321821034669
12	30.322882974752787	19.71045167301416	36.19436621526571	13.772299136967344
13	20.571833644059325	28.467514078959553	36.188955639699685	14.771696637281433
14	16.036670863683756	30.673286521155806	38.34511585509562	14.944926760064817
15	15.76595867604133	20.785688935923197	48.302959218015936	15.145393170019542
16	18.1833854980068	17.966870770695223	46.709590566157125	17.140153165140852
17	18.087554117218744	18.26445242682655	37.403675706607416	26.24431774934729
18	20.49195887595751	20.806872714834242	40.67744074273447	18.023727666473782
19	24.42691458718684	21.670180483962227	35.4531173627204	18.449787566130528
20	25.68583630532887	21.327113311208056	35.176811190170724	17.810239193292354
21	21.639367714637075	23.21797191452758	37.38340897440248	17.75925139643287
22	21.33004786066759	20.744513538819046	35.924479369658776	22.000959230854587
23	18.822291938150702	24.390141014272	35.38012044491438	21.40744660266292
24	18.860807899807146	21.57389057982112	42.248250045623074	17.31705147474866
25	18.305352709918868	21.810213515984582	40.197091678076255	19.68734209602029
26	17.02671448759557	25.972780219669367	36.76018403293298	20.240321259802084
27	17.49624240112173	26.0102874299491	37.77627178329819	18.71719838563098
28	16.9290490133953	23.617070641024817	41.7465337927126	17.707346552867286
29	17.630314629554398	22.011872086657245	41.03793180290465	19.3198814808837
30	19.59857197486925	24.19572711257757	38.573643894257174	17.632057018296
31	25.01162356699989	22.77769779092619	34.43831347774374	17.772365164330182
32	25.083611733429194	22.47846545072387	34.7353449058514	17.702577909995533
33	24.139328740152063	23.933635163972536	33.02028415609236	18.906751939783046
34	19.824073759900664	24.78492962125054	34.578254805095476	20.812741813753316
35	21.161311266744125	24.121538034053614	34.39915558339302	20.317995115809243
36	26.00331787498269	24.091550606763953	31.06440694130993	18.84072457694343
37	21.285020867397797	28.001562647587203	31.60179631108792	19.111620173927076
38	21.295933723200456	28.04200440732647	29.625010431406285	21.037051438066793
39	21.48026011112772	27.23885490211902	30.329302301695527	20.951582685057733
40	22.994395927578985	24.4933087687089	30.490610817299533	22.02168448641258
41	19.687617210032123	23.85678665000087	31.36354757684164	25.092048563125367
42	21.65486580397026	24.84994823271344	29.34907107753905	24.146114885777248
43	21.40753830733353	25.05105657536244	30.405233768961086	23.136171348342945
44	20.49957036361819	26.833887076702705	30.83312776203005	21.83341479764906
45	19.248993770501727	30.221090790375044	28.937867334521215	21.592048104602014
46	21.652298073193165	27.09166890578904	30.61679644405969	20.6392365769581
47	21.299143386671826	25.612472568840406	31.32154683770199	21.76683720678578
48	21.688704827425564	24.389957604930775	32.774974162209055	21.146363405434602
49	21.29318258308214	23.58579934834661	33.11290587340904	22.008112195162212
50	19.936503686069234	27.223907040809497	31.593909709415406	21.24567956370586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	463.0
1	970.0
2	1477.0
3	21828.5
4	42180.0
5	29229.5
6	16279.0
7	16275.5
8	16272.0
9	16718.0
10	17164.0
11	16923.0
12	16682.0
13	15834.5
14	14987.0
15	14009.0
16	13031.0
17	12093.0
18	11155.0
19	10228.0
20	9301.0
21	8831.5
22	8362.0
23	8368.0
24	8374.0
25	8238.5
26	8103.0
27	8561.5
28	9020.0
29	10467.5
30	11915.0
31	13776.0
32	15637.0
33	17659.0
34	19681.0
35	22460.0
36	25239.0
37	27292.0
38	29345.0
39	35627.0
40	41909.0
41	52811.0
42	63713.0
43	72168.5
44	80624.0
45	82238.5
46	83853.0
47	81722.0
48	79591.0
49	78577.5
50	77564.0
51	75779.0
52	73994.0
53	66752.5
54	59511.0
55	54528.5
56	49546.0
57	46488.0
58	43430.0
59	39780.5
60	36131.0
61	33392.0
62	30653.0
63	27150.5
64	23648.0
65	20050.0
66	16452.0
67	14192.0
68	11932.0
69	10214.5
70	8497.0
71	7273.0
72	6049.0
73	4880.0
74	3711.0
75	3024.0
76	2337.0
77	1843.0
78	1349.0
79	1000.0
80	651.0
81	485.5
82	320.0
83	231.5
84	143.0
85	126.5
86	110.0
87	72.5
88	35.0
89	27.5
90	20.0
91	14.5
92	9.0
93	7.0
94	5.0
95	3.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1090457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.852693581303882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5962216538012	24.07943284242859
2	7.445995694629332	4.7435003853747215
3	3.448505650314304	3.295325813785699
4	2.1937991128760923	2.795136436855138
5	1.5995971285315849	2.547573859432507
6	1.2287892151351212	2.348414780742594
7	0.9518979146672645	2.1224358817604974
8	0.802628693009645	2.0452708654398912
9	0.6609528896995757	1.8947816880550135
>10	5.397936901981912	35.37176664000348
>50	0.6103286400259234	13.011374095203815
>100	0.06247874498131194	3.081179164077231
>500	2.8925344893761213E-4	0.046343912426015786
>1k	2.8925344893761213E-4	0.11636652364623845
>5k	0.0	0.0
>10k+	2.8925344893761213E-4	2.5010971107686375
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27146	2.489414988394774	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1263	0.11582299898116111	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020816960228601404	0.0
2	9.170467061057887E-5	0.0	0.0	0.10445161982544933	0.0
3	9.170467061057887E-5	0.0	0.0	0.15553112135554176	0.0
4	9.170467061057887E-5	0.0	0.0	0.23714827819895695	0.0
5	9.170467061057887E-5	0.0	0.0	0.4376146881536824	0.0
6	9.170467061057887E-5	0.0	0.0	0.6698109141396681	0.0
7	9.170467061057887E-5	0.0	0.0	0.8247001028009358	0.0
8	9.170467061057887E-5	0.0	0.0	1.1878505984188281	0.0
9	9.170467061057887E-5	0.0	0.0	1.3961119053754527	0.0
10	9.170467061057887E-5	0.0	0.0	1.7426638556128302	0.0
11	9.170467061057887E-5	0.0	0.0	1.9995286379930617	0.0
12	9.170467061057887E-5	0.0	0.0	2.2460307925942975	0.0
13	1.8340934122115774E-4	0.0	0.0	2.3383773958991507	0.0
14	1.8340934122115774E-4	0.0	0.0	2.381386886415512	0.0
15	2.751140118317366E-4	0.0	0.0	2.4319161599219408	0.0
16	2.751140118317366E-4	0.0	0.0	2.5351756190294528	0.0
17	3.668186824423155E-4	0.0	0.0	2.6532912347758786	0.0
18	3.668186824423155E-4	0.0	0.0	2.8410106955157333	0.0
19	4.5852335305289433E-4	0.0	0.0	2.9180426188286197	0.0
20	4.5852335305289433E-4	0.0	0.0	3.013782294946064	0.0
21	5.502280236634732E-4	0.0	0.0	3.1140154999234264	0.0
22	5.502280236634732E-4	0.0	0.0	3.213423362865294	0.0
23	5.502280236634732E-4	0.0	0.0	3.3205344181384504	0.0
24	5.502280236634732E-4	0.0	0.0	3.4138897728200197	0.0
25	5.502280236634732E-4	0.0	0.0	3.4848691878726075	0.0
26	5.502280236634732E-4	0.0	0.0	3.5599753131026715	0.0
27	5.502280236634732E-4	0.0	0.0	3.647644978206385	0.0
28	6.419326942740521E-4	0.0	0.0	3.7296289537322425	0.0
29	6.419326942740521E-4	0.0	0.0	3.8159230487767974	0.0
30	6.419326942740521E-4	0.0	0.0	3.9303704776987995	1.8340934122115774E-4
31	6.419326942740521E-4	0.0	0.0	4.022625376333042	1.8340934122115774E-4
32	6.419326942740521E-4	0.0	0.0	4.111487202154693	1.8340934122115774E-4
33	7.33637364884631E-4	0.0	0.0	4.204659147495041	1.8340934122115774E-4
34	7.33637364884631E-4	0.0	0.0	4.300123709600654	1.8340934122115774E-4
35	7.33637364884631E-4	0.0	0.0	4.4150296618757086	1.8340934122115774E-4
36	8.253420354952098E-4	0.0	0.0	4.50554217176835	1.8340934122115774E-4
37	9.170467061057887E-4	0.0	0.0	4.603941283333501	1.8340934122115774E-4
38	9.170467061057887E-4	0.0	0.0	4.716738028184514	1.8340934122115774E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGTC	20	7.856302E-4	44.000004	34
ATACGAG	20	7.856302E-4	44.000004	1
TACGTCA	20	7.856302E-4	44.000004	35
CGTTTTT	16155	0.0	43.428043	1
CGGTCTA	125	0.0	40.48	31
TATAGCG	50	1.3478711E-9	39.600002	1
TATACGG	100	0.0	39.600002	2
GCGATAT	95	0.0	39.36842	9
GTTTTTT	18350	0.0	38.964577	2
TCACGAC	130	0.0	38.92308	25
CGACGGT	130	0.0	38.92308	28
ACGGTCT	130	0.0	38.92308	30
TCGTTGA	210	0.0	38.761906	24
TATGGGC	245	0.0	38.612244	4
ACGTAGG	115	0.0	38.26087	2
TAGGGCG	380	0.0	38.210526	5
TCTAGCG	35	7.289864E-6	37.714287	1
CGTAAGG	160	0.0	37.125004	2
ACACGTG	95	0.0	37.05263	42
CGTTGAT	220	0.0	37.0	25
>>END_MODULE