FastQCFastQC Report
Thu 26 May 2016
SRR1545974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545974_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences942065
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141751.5046732444151942No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT26570.28203998662512675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG254.4422275E-544.031
TACGTCA207.855758E-444.035
CGTTTTT149850.043.383381
CACGACG700.040.8571426
CGCATGG1300.040.6153872
ATAGGCG1150.040.1739164
TAACGGG1700.040.117653
CTCGAAT557.8216544E-1140.022
TAGGACG557.8216544E-1140.01
GTTTTTT168850.039.1400642
CGCACGG850.038.8235322
CGTTAGG1250.038.722
ACGGGTG404.1234853E-738.55
CGACGGT1100.038.028
ACGACGG1100.038.027
CGGTCTA1050.037.71428731
ACGGTCT1050.037.71428730
TCGCAAT357.2888106E-637.71428321
ATACGTT357.2888106E-637.71428311
TTAGCGG2350.037.446812
Produced by FastQC (version 0.11.5)