##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545974_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	942065
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.93048568835484	28.0	25.0	30.0	16.0	33.0
2	26.171092228243275	30.0	25.0	31.0	10.0	33.0
3	26.409454761614114	30.0	25.0	31.0	16.0	33.0
4	27.820807481437054	32.0	19.0	35.0	10.0	35.0
5	31.161622605658845	33.0	30.0	35.0	19.0	37.0
6	29.933134125564585	33.0	28.0	35.0	17.0	36.0
7	29.925751407811564	32.0	28.0	35.0	17.0	35.0
8	32.39572428654074	33.0	32.0	35.0	28.0	35.0
9	27.159981529936893	31.0	23.0	34.0	10.0	35.0
10	31.3727449804419	34.0	28.0	37.0	17.0	39.0
11	33.692751561728755	35.0	32.0	37.0	27.0	39.0
12	33.257592628958726	35.0	31.0	39.0	24.0	39.0
13	33.53724849134614	35.0	32.0	39.0	25.0	39.0
14	33.346466538933086	36.0	31.0	39.0	22.0	40.0
15	32.14516620403051	34.0	27.0	38.0	18.0	40.0
16	30.652928407275507	34.0	27.0	38.0	17.0	40.0
17	30.921734699834936	33.0	27.0	38.0	17.0	40.0
18	31.783988365983237	34.0	29.0	38.0	18.0	39.0
19	31.393315747851794	34.0	27.0	37.0	18.0	39.0
20	32.01318380366535	34.0	30.0	37.0	21.0	39.0
21	32.85304411054439	35.0	31.0	37.0	24.0	39.0
22	33.200726064549684	35.0	31.0	37.0	25.0	39.0
23	34.40275458699771	35.0	33.0	38.0	28.0	40.0
24	33.995337901312546	35.0	33.0	38.0	26.0	40.0
25	32.811213663600704	35.0	31.0	38.0	23.0	39.0
26	26.420407296736425	33.0	20.0	36.0	0.0	39.0
27	29.723785513738438	34.0	24.0	37.0	15.0	39.0
28	31.249386188851087	34.0	27.0	37.0	20.0	39.0
29	32.47840117189366	35.0	30.0	37.0	23.0	39.0
30	32.31658537362072	35.0	30.0	37.0	22.0	39.0
31	31.147173496520942	34.0	29.0	36.0	18.0	39.0
32	31.407400763216977	34.0	30.0	37.0	18.0	39.0
33	31.013681646170912	34.0	29.0	37.0	17.0	39.0
34	30.810820909385235	34.0	29.0	37.0	15.0	39.0
35	29.937442745458117	34.0	27.0	37.0	10.0	39.0
36	29.67107046753674	34.0	26.0	37.0	10.0	39.0
37	29.73870698943279	34.0	26.0	37.0	10.0	39.0
38	29.47129019759783	33.0	26.0	37.0	10.0	39.0
39	29.299088704070314	33.0	25.0	37.0	9.0	39.0
40	29.182319691316415	33.0	25.0	36.0	9.0	38.0
41	28.56452049487031	33.0	23.0	36.0	8.0	38.0
42	28.697624898494265	33.0	24.0	36.0	8.0	38.0
43	28.29935195554447	32.0	23.0	36.0	8.0	38.0
44	28.090086140552934	32.0	23.0	35.0	8.0	38.0
45	27.604113304283675	31.0	23.0	35.0	8.0	38.0
46	27.17808537627446	31.0	22.0	35.0	7.0	38.0
47	27.201848067808484	31.0	23.0	35.0	8.0	38.0
48	27.253305239022787	31.0	23.0	34.0	8.0	37.0
49	27.06889758137708	31.0	22.0	34.0	7.0	37.0
50	28.819594189360608	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	12.0
11	22.0
12	32.0
13	131.0
14	313.0
15	730.0
16	1571.0
17	2813.0
18	4545.0
19	7335.0
20	10685.0
21	14756.0
22	19823.0
23	26368.0
24	34002.0
25	42270.0
26	49286.0
27	52859.0
28	57398.0
29	64979.0
30	76372.0
31	89032.0
32	97647.0
33	97894.0
34	87362.0
35	65666.0
36	31712.0
37	6304.0
38	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.143700275458702	22.032237690605214	25.770090174244874	27.053971859691213
2	41.80486484478247	16.759777722343998	28.420119630811037	13.01523780206249
3	28.604501812507632	14.161761661881082	43.13821233142087	14.095524194190423
4	25.674343065499727	23.6356302378286	36.907219777828495	13.78280691884318
5	19.43103713650332	27.946797726271544	39.57826689241188	13.043898244813256
6	18.10798617929761	33.34557594221206	38.11966265597385	10.426775222516493
7	74.92890617950991	3.5153625280633505	19.860094579461077	1.6956367129656658
8	76.58961961223483	2.303981147797657	19.313317021649247	1.793082218318269
9	64.40521620058064	3.4691873702982283	19.18997096803299	12.93562546108814
10	40.74453461279211	22.14061662411829	26.302537510681322	10.812311252408273
11	34.718411149973726	20.45962858189191	32.27431228206122	12.547647986073148
12	30.715184196419564	19.590049518876086	36.81179111844724	12.882975166257104
13	19.888755022211843	29.16433579423925	36.58229527686518	14.36461390668372
14	15.350851586673956	30.758811759273513	39.22139130527087	14.668945348781664
15	14.438918758259781	19.12001825776353	48.51278839570518	17.92827458827151
16	16.355347030194306	16.057278425586343	46.822034572985935	20.765339971233406
17	17.406123781267745	17.354004235376543	38.13388672756126	27.10598525579445
18	20.034498681088884	20.262189976275522	41.87970044529836	17.823610897337232
19	24.909533843206148	20.879026394144777	36.25057718947207	17.96086257317701
20	26.097456120331397	20.768206015508483	35.88648341674938	17.24785444741074
21	21.340777971796	22.8125447819418	38.27517209534374	17.571505150918462
22	21.044832362947353	20.199349301799767	36.349614941644155	22.40620339360872
23	18.28217798134948	24.19291662464904	36.259918370813054	21.26498702318842
24	18.13494822544092	20.88295393629951	44.241533227537374	16.74056461072219
25	17.841762511079384	20.539983971382018	41.83331298795731	19.78494052958129
26	13.449496584630571	38.215091315355096	30.760404006092994	17.575008093921333
27	16.74183840817778	26.570565725294966	38.42080960443282	18.266786262094442
28	16.277963834767238	23.218567720910976	43.28745893330078	17.216009511021003
29	16.94543370149618	21.693938316358214	42.15462839612978	19.205999586015825
30	19.056540684559984	24.070844368488373	39.3274349434487	17.545180003502946
31	25.364810283791456	22.427008752050018	35.00958001836391	17.198600945794613
32	24.94488172259876	21.856559791521814	35.47324229219852	17.725316193680904
33	24.008322143376517	23.589773529427376	33.748414387542255	18.653489939653845
34	19.135622276594503	24.297898764947217	34.85980266754417	21.70667629091411
35	20.726382999049957	23.536167886504646	34.80598472504551	20.93146438939988
36	25.972517819895657	23.4313980457824	31.04711458338862	19.54896955093332
37	20.373859553215542	28.25983345098268	31.573829831274914	19.792477164526865
38	20.54348691438489	28.35791585506308	29.739030746286083	21.359566484265947
39	20.829560592952713	27.25969014876893	30.314256447272747	21.59649281100561
40	22.79630386438303	23.956627196637175	30.595765684958042	22.65130325402175
41	18.957609082175857	22.957863841666974	31.494323640088528	26.590203436068638
42	21.04270936718804	24.223912362735057	28.62668711819248	26.106691151884426
43	20.882317037571717	24.265629229405615	30.53876324882041	24.31329048420226
44	19.77613009718013	26.254027057580952	30.915382696523064	23.05446014871585
45	18.534814476708085	30.993615090253858	28.363647943613234	22.107922489424826
46	20.9006809508898	26.5294857573522	30.405545264923333	22.164288026834665
47	20.671503558671642	25.02820930615191	30.808914459193364	23.49137267598308
48	20.942503967348326	23.78540758864834	33.23231411845255	22.039774325550784
49	20.802067797869576	23.287140483936884	32.67407238354041	23.23671933465313
50	19.351849394680833	27.481755505193377	31.557482763928178	21.60891233619761
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	252.0
1	587.5
2	923.0
3	12718.0
4	24513.0
5	19445.5
6	14378.0
7	13992.5
8	13607.0
9	14447.0
10	15287.0
11	15708.0
12	16129.0
13	15780.5
14	15432.0
15	14834.5
16	14237.0
17	13561.5
18	12886.0
19	12086.5
20	11287.0
21	10534.0
22	9781.0
23	9724.0
24	9667.0
25	9079.5
26	8492.0
27	8707.5
28	8923.0
29	10027.5
30	11132.0
31	12623.0
32	14114.0
33	15662.5
34	17211.0
35	19262.5
36	21314.0
37	23067.0
38	24820.0
39	29576.0
40	34332.0
41	43282.0
42	52232.0
43	59484.0
44	66736.0
45	68804.0
46	70872.0
47	68891.5
48	66911.0
49	65670.0
50	64429.0
51	62782.5
52	61136.0
53	55780.0
54	50424.0
55	47065.5
56	43707.0
57	40906.5
58	38106.0
59	35126.0
60	32146.0
61	29692.5
62	27239.0
63	24196.0
64	21153.0
65	18167.5
66	15182.0
67	13125.0
68	11068.0
69	9486.0
70	7904.0
71	6857.5
72	5811.0
73	4674.0
74	3537.0
75	2855.0
76	2173.0
77	1721.0
78	1269.0
79	946.5
80	624.0
81	480.5
82	337.0
83	239.5
84	142.0
85	126.0
86	110.0
87	76.0
88	42.0
89	36.0
90	30.0
91	24.0
92	18.0
93	11.0
94	4.0
95	5.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	942065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.65529556296332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.01807124770319	39.90613498061182
2	7.104907583196566	6.913827568159365
3	2.9227325649141878	4.26619250392193
4	1.6645199309836607	3.2395083684981327
5	1.1128693771229043	2.7073494233442905
6	0.7641241713571425	2.230721244251172
7	0.5598260217894591	1.9066950387802852
8	0.4679017526581908	1.8212718456010266
9	0.3794541678812546	1.6616209221774672
>10	2.826246984579318	27.053295024538933
>50	0.160946150868714	4.941485351566872
>100	0.017468398999573056	1.323057880696719
>500	4.658239728972228E-4	0.12137002050211895
>1k	2.329119864486114E-4	0.3011019089394305
>5k	0.0	0.0
>10k+	2.329119864486114E-4	1.606367918410398
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14175	1.5046732444151942	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	2657	0.28203998662512675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020805358441296513	0.0
2	0.0	0.0	0.0	0.09118266786262094	0.0
3	0.0	0.0	0.0	0.13640247753605111	0.0
4	0.0	0.0	0.0	0.189158922155053	0.0
5	0.0	0.0	0.0	0.3135664736509689	0.0
6	0.0	0.0	0.0	0.44784595542770406	0.0
7	0.0	0.0	0.0	0.5397716718060855	0.0
8	0.0	0.0	0.0	0.7717089585113553	0.0
9	0.0	0.0	0.0	0.9377272268898643	0.0
10	0.0	0.0	0.0	1.1751843025693556	0.0
11	0.0	0.0	0.0	1.3629632774808533	0.0
12	0.0	0.0	0.0	1.519534214730406	0.0
13	0.0	0.0	0.0	1.586408581148859	0.0
14	0.0	0.0	0.0	1.6126275787764115	0.0
15	0.0	0.0	0.0	1.64702011007733	0.0
16	0.0	0.0	0.0	1.7103915334929118	0.0
17	0.0	0.0	0.0	1.8021049502953619	0.0
18	0.0	0.0	0.0	1.9224788098485772	0.0
19	0.0	0.0	0.0	1.9713077123128446	0.0
20	0.0	0.0	0.0	2.0299023952699655	0.0
21	0.0	0.0	0.0	2.083401888404728	0.0
22	0.0	0.0	0.0	2.150700853975044	0.0
23	0.0	0.0	0.0	2.2174690706055316	0.0
24	0.0	0.0	0.0	2.273516158651473	0.0
25	0.0	0.0	0.0	2.3117300823191607	0.0
26	0.0	0.0	0.0	2.356312993264796	0.0
27	0.0	0.0	0.0	2.4193659673164802	0.0
28	0.0	0.0	0.0	2.4788098485773276	0.0
29	0.0	0.0	0.0	2.5451534660559516	0.0
30	0.0	0.0	0.0	2.626570353425719	2.1229957593159709E-4
31	0.0	0.0	0.0	2.693975468784001	2.1229957593159709E-4
32	0.0	0.0	0.0	2.7647773773571886	2.1229957593159709E-4
33	0.0	0.0	0.0	2.8368530833859658	2.1229957593159709E-4
34	0.0	0.0	0.0	2.908398040474914	2.1229957593159709E-4
35	0.0	0.0	0.0	2.9940609193633136	2.1229957593159709E-4
36	0.0	0.0	0.0	3.0648628279365013	2.1229957593159709E-4
37	0.0	0.0	0.0	3.141502974847808	2.1229957593159709E-4
38	0.0	0.0	0.0	3.2280150520399333	2.1229957593159709E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	25	4.4422275E-5	44.0	31
TACGTCA	20	7.855758E-4	44.0	35
CGTTTTT	14985	0.0	43.38338	1
CACGACG	70	0.0	40.85714	26
CGCATGG	130	0.0	40.615387	2
ATAGGCG	115	0.0	40.173916	4
TAACGGG	170	0.0	40.11765	3
CTCGAAT	55	7.8216544E-11	40.0	22
TAGGACG	55	7.8216544E-11	40.0	1
GTTTTTT	16885	0.0	39.140064	2
CGCACGG	85	0.0	38.823532	2
CGTTAGG	125	0.0	38.72	2
ACGGGTG	40	4.1234853E-7	38.5	5
CGACGGT	110	0.0	38.0	28
ACGACGG	110	0.0	38.0	27
CGGTCTA	105	0.0	37.714287	31
ACGGTCT	105	0.0	37.714287	30
TCGCAAT	35	7.2888106E-6	37.714283	21
ATACGTT	35	7.2888106E-6	37.714283	11
TTAGCGG	235	0.0	37.44681	2
>>END_MODULE