FastQCFastQC Report
Thu 26 May 2016
SRR1545973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545973_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences160773
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21661.3472411412364016No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA1900.11817904747687734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT551.8189894E-1244.0000049
GTGTAGG551.8189894E-1244.0000042
TCACGGG207.8362663E-444.03
GTTGATC254.4257205E-544.016
TTGATCC254.4257205E-544.017
GTTGAAG454.7475623E-1044.01
AAGTAGA207.8362663E-444.028
GGAATAC207.8362663E-444.08
TCGTTTC207.8362663E-444.041
TCGTTGA408.236384E-944.024
AATTTCG207.8362663E-444.037
CGAAAGG207.8362663E-444.02
CGACGGG207.8362663E-444.03
GGCACGG207.8362663E-444.02
ACCGATT207.8362663E-444.010
TGTATCA207.8362663E-444.019
CTAAGTT207.8362663E-444.021
GAATACT207.8362663E-444.09
CCTAAGT207.8362663E-444.020
GCGTTGG207.8362663E-444.02
Produced by FastQC (version 0.11.5)