##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545973_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	160773
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.152929907384948	31.0	28.0	33.0	25.0	33.0
2	28.73811523079124	31.0	28.0	33.0	16.0	33.0
3	28.216622194025117	31.0	27.0	33.0	16.0	34.0
4	32.16248997033084	35.0	32.0	35.0	25.0	37.0
5	33.6539904088373	35.0	33.0	35.0	28.0	37.0
6	33.42925740018536	35.0	33.0	37.0	28.0	37.0
7	34.42846746655222	35.0	35.0	37.0	32.0	37.0
8	34.819577914202014	35.0	35.0	37.0	32.0	37.0
9	36.658132895448865	39.0	37.0	39.0	32.0	39.0
10	36.48274274909344	39.0	35.0	39.0	32.0	39.0
11	36.37687920235363	39.0	35.0	39.0	32.0	39.0
12	34.927730402492955	35.0	33.0	39.0	29.0	39.0
13	34.57517742406996	37.0	33.0	39.0	25.0	39.0
14	35.31086065446313	37.0	33.0	40.0	25.0	41.0
15	36.09432553973615	38.0	34.0	40.0	30.0	41.0
16	35.78554234852867	36.0	34.0	40.0	30.0	41.0
17	35.310344398624146	36.0	33.0	40.0	29.0	41.0
18	34.98969354307004	36.0	33.0	39.0	29.0	40.0
19	34.446293842871626	36.0	33.0	39.0	27.0	40.0
20	34.899585129343855	35.0	34.0	39.0	29.0	40.0
21	35.06473101826799	35.0	34.0	39.0	30.0	40.0
22	35.40964589825406	35.0	34.0	39.0	30.0	40.0
23	35.7959483246565	35.0	34.0	40.0	31.0	41.0
24	35.48223271320433	35.0	34.0	40.0	31.0	41.0
25	35.1122203355041	35.0	34.0	39.0	30.0	41.0
26	35.210725681551004	35.0	34.0	39.0	30.0	41.0
27	35.45846006481188	35.0	34.0	40.0	31.0	41.0
28	35.43595006624247	36.0	34.0	40.0	31.0	41.0
29	35.623226536794114	36.0	35.0	40.0	31.0	41.0
30	35.25146635318119	36.0	34.0	40.0	30.0	41.0
31	34.604131290701794	35.0	34.0	39.0	29.0	40.0
32	34.46130258190119	35.0	34.0	39.0	27.0	40.0
33	34.1177374310363	35.0	33.0	39.0	24.0	41.0
34	33.82434239580029	35.0	34.0	40.0	23.0	41.0
35	33.5466651738787	35.0	33.0	40.0	21.0	41.0
36	33.12856014380524	35.0	33.0	39.0	18.0	41.0
37	33.01389536800333	35.0	33.0	39.0	18.0	41.0
38	32.86347210041487	35.0	33.0	39.0	18.0	40.0
39	32.73187040112457	35.0	33.0	39.0	17.0	40.0
40	32.55610705777711	35.0	32.0	39.0	16.0	40.0
41	32.26875781381202	35.0	32.0	38.0	15.0	40.0
42	32.412444875694305	35.0	32.0	39.0	15.0	40.0
43	32.20115317870538	35.0	32.0	38.0	15.0	40.0
44	31.891636033413572	35.0	31.0	38.0	12.0	40.0
45	31.86056116387702	35.0	31.0	38.0	12.0	40.0
46	31.736784161519658	35.0	31.0	38.0	12.0	40.0
47	31.74296679168766	35.0	31.0	38.0	12.0	40.0
48	31.84062622455263	35.0	31.0	38.0	12.0	40.0
49	31.863378801166863	35.0	31.0	38.0	12.0	40.0
50	31.94703090693089	35.0	32.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	5.0
15	16.0
16	37.0
17	91.0
18	194.0
19	355.0
20	545.0
21	742.0
22	1127.0
23	1554.0
24	2210.0
25	3168.0
26	4514.0
27	5171.0
28	5138.0
29	4845.0
30	5440.0
31	6684.0
32	9145.0
33	14729.0
34	24290.0
35	15032.0
36	13335.0
37	18401.0
38	19636.0
39	4366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.77982621460071	23.86656963544874	27.923842933825956	22.429761216124597
2	36.17336244269872	20.93386327306202	30.415554850627903	12.477219433611365
3	27.61346743545247	19.037400558551496	40.1566183376562	13.192513668339833
4	24.37784951453291	25.213810776685143	36.29900543001624	14.109334278765715
5	20.135843705099738	29.483184365534015	37.25127975468518	13.12969217468107
6	17.963837211472075	37.17664035627873	34.37019897619626	10.489323456052944
7	77.80535288885572	4.780031472946328	15.103282267544923	2.3113333706530326
8	79.64832403450828	3.3936046475465407	14.575830518806018	2.382240799139159
9	76.72805757185597	4.548027342899616	15.661833765619848	3.0620813196245638
10	52.246956889527475	19.124479856692357	19.539350512834865	9.089212740945309
11	47.09621640449578	16.270144862632407	26.24818843960118	10.385450293270635
12	41.860884601270115	18.79544450871726	27.916378993985308	11.427291896027318
13	18.96213916515833	40.13733649306786	28.40962101845459	12.490903323319213
14	13.166389878897574	40.61254066292226	32.55957156985315	13.66149788832702
15	11.970915514420954	20.10971991565748	54.89478954799625	13.024575021925322
16	14.216939411468344	15.108258227438686	53.06114832714448	17.613654033948485
17	15.2793068487868	15.921205675082259	33.916764630877076	34.882722845253866
18	19.975990993512593	19.986564908286837	40.66230026185989	19.37514383634068
19	27.428734924396508	21.46753497166813	31.57246552592786	19.5312645780075
20	29.599497428050732	20.35851791034564	30.7172224191873	19.324762242416327
21	20.113451885577803	26.941090854807708	34.01006387888514	18.93539338072935
22	21.419019362703935	21.767958550254086	30.510720083596127	26.302302003445853
23	17.505426906259135	28.13283324936401	30.462204474631932	23.89953536974492
24	17.554564510210046	20.483538902676443	45.833566581453354	16.128330005660153
25	15.487675169338136	21.782264434948654	43.18386793802442	19.54619245768879
26	14.843910358082512	31.84303334515124	33.86949301188633	19.443563284879925
27	15.712215359544201	32.22369427702413	34.578567296747586	17.485523066684085
28	13.50786512660708	25.27414429039702	44.86760836707656	16.35038221591934
29	14.350046338626509	21.64107157296312	43.63232632344983	20.376555764960536
30	17.000366977042162	28.789659955340756	36.67095843207503	17.53901463554204
31	28.31196780553949	23.785088292188362	30.47899833927338	17.423945562998764
32	29.81035372854895	22.942285085182213	30.944872584326973	16.30248860194187
33	27.37462136055183	24.826929894945046	29.52734600959116	18.271102734911956
34	18.38181784254819	25.47567066609443	31.720500332767315	24.422011158590063
35	19.170507485709663	24.6030116997257	35.16759654917181	21.058884265392823
36	30.401870960920057	22.788030328475553	28.599951484391035	18.21014722621336
37	19.717241079036903	31.331753466067063	30.91128485504407	18.039720599851965
38	19.88144775553109	31.29816573678416	27.019462223134482	21.800924284550266
39	20.1439296399271	30.51196407356957	29.118695303315857	20.225410983187476
40	24.581241875190486	24.481722677315222	27.207304709124045	23.72973073837025
41	17.778482705429393	22.498180664663842	29.84456345281857	29.878773177088192
42	21.808388224390914	23.033096353243394	26.483302544581488	28.675212877784205
43	21.888625577677846	24.520286366491888	27.762124237278645	25.82896381855162
44	19.150603646134613	28.643491133461463	29.970206440136092	22.235698780267832
45	16.596070235673903	36.80779732915352	24.772816331100373	21.8233161040722
46	21.191369197564267	31.336729425960826	27.242758423367107	20.229142953107797
47	21.190747202577548	25.54720008956728	29.34136950856176	23.920683199293414
48	22.256224614829602	21.92843325682795	33.51495586945569	22.300386258886753
49	20.668271413732406	22.576552032990612	34.304889502590605	22.45028705068637
50	18.416649561804533	31.128361105409493	29.11247535344865	21.342513979337326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	120.0
2	159.0
3	1801.0
4	3443.0
5	2551.0
6	1659.0
7	1744.0
8	1829.0
9	1986.0
10	2143.0
11	2167.0
12	2191.0
13	2061.5
14	1932.0
15	1809.0
16	1686.0
17	1540.5
18	1395.0
19	1273.5
20	1152.0
21	1039.5
22	927.0
23	902.5
24	878.0
25	874.5
26	871.0
27	972.5
28	1074.0
29	1293.0
30	1512.0
31	2014.0
32	2516.0
33	2535.0
34	2554.0
35	2972.5
36	3391.0
37	3736.5
38	4082.0
39	5461.5
40	6841.0
41	9657.5
42	12474.0
43	14952.5
44	17431.0
45	16982.5
46	16534.0
47	15078.5
48	13623.0
49	12755.5
50	11888.0
51	11213.5
52	10539.0
53	9336.0
54	8133.0
55	7109.0
56	6085.0
57	5487.0
58	4889.0
59	4464.0
60	4039.0
61	3653.0
62	3267.0
63	3058.0
64	2849.0
65	2531.0
66	2213.0
67	1887.5
68	1562.0
69	1326.5
70	1091.0
71	872.0
72	653.0
73	592.5
74	532.0
75	446.0
76	360.0
77	239.0
78	118.0
79	105.0
80	92.0
81	63.0
82	34.0
83	31.0
84	28.0
85	22.0
86	16.0
87	9.0
88	2.0
89	2.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	160773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.459194018896206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.76005951610817	27.98293245756439
2	8.579699831802303	6.599366809103518
3	3.8798680294992884	4.47649791942677
4	2.505175313753396	3.853880937719642
5	1.7353473929356968	3.337003103754984
6	1.3827791434855738	3.1908342818756883
7	1.1692974511579766	3.14791662779198
8	0.9444947599948246	2.9059605779577415
9	0.8878897658170527	3.073277229385531
>10	6.124660370034933	39.343670890012625
>50	0.027493854314917843	0.6232389766938479
>100	0.0016172855479363434	0.11817904747687734
>500	0.0	0.0
>1k	0.0016172855479363434	1.3472411412364016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2166	1.3472411412364016	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	190	0.11817904747687734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041051669123546866	0.0
2	0.0	0.0	0.0	0.10884912267607123	0.0
3	0.0	0.0	0.0	0.1418148569722528	0.0
4	0.0	0.0	0.0	0.22640617516622816	0.0
5	0.0	0.0	0.0	0.3794169418994483	0.0
6	0.0	0.0	0.0	0.5971151872515907	0.0
7	0.0	0.0	0.0	0.7339540843300802	0.0
8	0.0	0.0	0.0	1.123322946017055	0.0
9	0.0	0.0	0.0	1.3198733618207037	0.0
10	6.21994986720407E-4	0.0	0.0	1.6731665142778949	0.0
11	6.21994986720407E-4	0.0	0.0	1.8809128398425108	0.0
12	6.21994986720407E-4	0.0	0.0	2.0681333308453533	0.0
13	6.21994986720407E-4	0.0	0.0	2.1496146741057265	0.0
14	6.21994986720407E-4	0.0	0.0	2.1794704334683064	0.0
15	6.21994986720407E-4	0.0	0.0	2.2186561176316917	0.0
16	6.21994986720407E-4	0.0	0.0	2.3051134207858284	0.0
17	6.21994986720407E-4	0.0	0.0	2.3953026938602875	0.0
18	6.21994986720407E-4	0.0	0.0	2.5613753553146363	0.0
19	6.21994986720407E-4	0.0	0.0	2.619220889079634	0.0
20	6.21994986720407E-4	0.0	0.0	2.6957262724462443	0.0
21	6.21994986720407E-4	0.0	0.0	2.794623475334789	0.0
22	6.21994986720407E-4	0.0	0.0	2.8960086581702154	0.0
23	6.21994986720407E-4	0.0	0.0	2.9911738911384376	0.0
24	6.21994986720407E-4	0.0	0.0	3.0552393747706392	0.0
25	6.21994986720407E-4	0.0	0.0	3.1168168784559596	0.0
26	6.21994986720407E-4	0.0	0.0	3.1653324874201516	0.0
27	6.21994986720407E-4	0.0	0.0	3.2206900412382677	0.0
28	6.21994986720407E-4	0.0	0.0	3.2729376201227818	0.0
29	6.21994986720407E-4	0.0	0.0	3.337625098741704	0.0
30	6.21994986720407E-4	0.0	0.0	3.434656316670088	0.0
31	6.21994986720407E-4	0.0	0.0	3.521113619824224	0.0
32	6.21994986720407E-4	0.0	0.0	3.5752271836688996	0.0
33	6.21994986720407E-4	0.0	0.0	3.6455126171683054	0.0
34	6.21994986720407E-4	0.0	0.0	3.734457900269324	0.0
35	6.21994986720407E-4	0.0	0.0	3.8314891181977075	0.0
36	6.21994986720407E-4	0.0	0.0	3.8974205867900706	0.0
37	0.001243989973440814	0.0	0.0	3.9863658698910887	0.0
38	0.001243989973440814	0.0	0.0	4.077177137952268	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGAT	55	1.8189894E-12	44.000004	9
GTGTAGG	55	1.8189894E-12	44.000004	2
TCACGGG	20	7.8362663E-4	44.0	3
GTTGATC	25	4.4257205E-5	44.0	16
TTGATCC	25	4.4257205E-5	44.0	17
GTTGAAG	45	4.7475623E-10	44.0	1
AAGTAGA	20	7.8362663E-4	44.0	28
GGAATAC	20	7.8362663E-4	44.0	8
TCGTTTC	20	7.8362663E-4	44.0	41
TCGTTGA	40	8.236384E-9	44.0	24
AATTTCG	20	7.8362663E-4	44.0	37
CGAAAGG	20	7.8362663E-4	44.0	2
CGACGGG	20	7.8362663E-4	44.0	3
GGCACGG	20	7.8362663E-4	44.0	2
ACCGATT	20	7.8362663E-4	44.0	10
TGTATCA	20	7.8362663E-4	44.0	19
CTAAGTT	20	7.8362663E-4	44.0	21
GAATACT	20	7.8362663E-4	44.0	9
CCTAAGT	20	7.8362663E-4	44.0	20
GCGTTGG	20	7.8362663E-4	44.0	2
>>END_MODULE