##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545971_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 159228 Sequences flagged as poor quality 0 Sequence length 50 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.029580224583615 31.0 28.0 33.0 25.0 33.0 2 28.590543120556685 31.0 28.0 33.0 16.0 33.0 3 28.091447484110834 31.0 26.0 33.0 16.0 34.0 4 32.05059411661266 35.0 32.0 35.0 25.0 37.0 5 33.61163237621524 35.0 33.0 35.0 28.0 37.0 6 33.445895194312556 35.0 33.0 37.0 28.0 37.0 7 34.40030019845756 35.0 35.0 37.0 32.0 37.0 8 34.82753033386088 35.0 35.0 37.0 32.0 37.0 9 36.74852412892205 39.0 37.0 39.0 32.0 39.0 10 36.550700881754466 39.0 35.0 39.0 32.0 39.0 11 36.40950084156053 39.0 35.0 39.0 32.0 39.0 12 35.02693621724822 35.0 34.0 39.0 30.0 39.0 13 34.67814705956239 37.0 33.0 39.0 26.0 39.0 14 35.3578516341347 37.0 33.0 40.0 26.0 41.0 15 36.11757982264426 38.0 34.0 40.0 31.0 41.0 16 35.78003240636069 36.0 34.0 40.0 30.0 41.0 17 35.31197402466903 36.0 33.0 40.0 29.0 41.0 18 34.98661039515663 36.0 33.0 39.0 29.0 40.0 19 34.395100108021204 36.0 32.0 39.0 27.0 40.0 20 34.9219169995227 35.0 34.0 39.0 29.0 40.0 21 35.011612279247366 35.0 34.0 39.0 30.0 40.0 22 35.37730173085136 35.0 34.0 39.0 30.0 40.0 23 35.782444042505084 35.0 34.0 40.0 31.0 41.0 24 35.384354510513226 35.0 34.0 39.0 31.0 41.0 25 35.047523048710026 35.0 34.0 39.0 30.0 41.0 26 35.139027055542996 35.0 34.0 39.0 30.0 41.0 27 35.44037480845078 35.0 34.0 40.0 31.0 41.0 28 35.428184741377144 36.0 34.0 40.0 31.0 41.0 29 35.60226216494586 36.0 35.0 40.0 31.0 41.0 30 35.256914613007766 36.0 34.0 40.0 30.0 41.0 31 34.56816012259151 35.0 34.0 39.0 29.0 40.0 32 34.46005099605597 35.0 34.0 39.0 27.0 41.0 33 34.1625656291607 35.0 33.0 40.0 24.0 41.0 34 33.81083728992388 35.0 34.0 40.0 22.0 41.0 35 33.521371869269224 35.0 33.0 40.0 20.0 41.0 36 33.12684954907429 35.0 33.0 39.0 18.0 41.0 37 33.03193533800588 35.0 33.0 40.0 18.0 41.0 38 32.89334162333258 35.0 33.0 39.0 18.0 40.0 39 32.697779285050366 35.0 33.0 39.0 17.0 40.0 40 32.54580852613862 35.0 32.0 39.0 16.0 40.0 41 32.25741703720451 35.0 32.0 38.0 15.0 40.0 42 32.451779837716984 35.0 32.0 39.0 15.0 40.0 43 32.22239179038863 35.0 32.0 38.0 15.0 40.0 44 31.914192227497676 35.0 31.0 38.0 11.0 40.0 45 31.87532971587912 35.0 31.0 38.0 12.0 40.0 46 31.75379330268546 35.0 31.0 38.0 12.0 40.0 47 31.785967292184793 35.0 31.0 38.0 12.0 40.0 48 31.85665209636496 35.0 31.0 38.0 12.0 40.0 49 31.95747607204763 35.0 32.0 38.0 12.0 40.0 50 31.968931343733516 35.0 32.0 38.0 12.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 1.0 14 5.0 15 12.0 16 27.0 17 98.0 18 191.0 19 302.0 20 491.0 21 787.0 22 1081.0 23 1577.0 24 2232.0 25 3211.0 26 4647.0 27 5160.0 28 5069.0 29 4853.0 30 5290.0 31 6477.0 32 9038.0 33 14436.0 34 24117.0 35 14838.0 36 13294.0 37 17951.0 38 19720.0 39 4320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.58406812872108 23.86577737583842 27.894591403521996 22.655563091918506 2 36.36546336071545 20.89582234280403 30.419272992187302 12.319441304293214 3 27.51526113497626 19.01675584696159 40.406209963071824 13.061773054990327 4 24.302258396764387 25.303966639033337 36.23860125103625 14.155173713166025 5 20.16793528776346 29.17137689351119 37.45760795839927 13.203079860326072 6 18.149446077323084 36.87102770869445 34.58813776471475 10.391388449267716 7 77.23641570578039 4.938201823799834 15.366644057577814 2.4587384128419627 8 79.30075112417414 3.3360966664154543 14.921370613208731 2.441781596201673 9 76.51920516492075 4.476599593036401 15.980857638103851 3.0233376039390056 10 52.23829979651819 18.874821011379908 19.70193684527847 9.184942346823423 11 47.12173738287236 16.290476549350615 26.347752907780038 10.240033159996985 12 41.755218931343734 18.789408897932525 28.034014118119927 11.421358052603814 13 19.121008867787072 39.79136835229984 28.715426934961187 12.372195844951893 14 13.252694249755068 40.432587233401165 32.670761423870175 13.643957092973597 15 12.169970105760292 19.95628909488281 54.92250106765142 12.95123973170548 16 14.16773431808476 14.967844851408044 53.25194061345995 17.612480217047253 17 15.118572110432837 15.786796292109429 34.14726053206722 34.947371065390506 18 19.775416384053056 20.21064134448715 40.678147059562384 19.335795211897405 19 27.224483131107597 21.226166252166703 31.924661491697442 19.62468912502826 20 29.413796568442734 20.140301956942245 30.89343582786947 19.55246564674555 21 20.161026955058155 26.613409701811243 34.36518702740724 18.860376315723364 22 21.493079106689777 21.65950712186299 30.747104780566232 26.100308990881 23 17.398949933428796 28.232471675835907 30.56120782776899 23.807370562966312 24 17.365036300148216 20.29354133695079 46.31534654708971 16.02607581581129 25 15.479689501846408 21.461049564147007 43.600371793905595 19.458889140100986 26 14.843494862712589 31.3858115406838 34.15605295551033 19.614640641093274 27 15.776747808174441 32.103650111789385 34.63963624488156 17.47996583515462 28 13.827341924787099 25.355465119200144 44.857688346270756 15.959504609742007 29 14.381264601703217 21.39761850930741 43.63679754816992 20.584319340819455 30 16.739518175195318 28.57223603888763 36.851558771070415 17.836687014846635 31 28.190393649358153 23.70814178410832 30.783530534830554 17.31793403170297 32 29.52935413369508 22.820735046599843 31.330544879041373 16.3193659406637 33 27.311779335292783 24.80342653302183 29.92815333986485 17.956640791820533 34 18.530032406360693 25.455949958550008 31.80470771472354 24.209309920365765 35 19.148642198608286 24.477478835380712 35.459215715828876 20.914663250182127 36 30.53859873891527 22.571407038962995 28.620594367824754 18.26939985429698 37 19.79865350315271 31.39460396412691 30.800487351470846 18.00625518124953 38 19.970733790539352 31.06488808501017 27.28477403471751 21.679604089732962 39 20.13402165448288 30.306227547918706 29.44959429246112 20.110156505137287 40 24.542165950712185 24.399603084884568 27.35636948275429 23.701861481648955 41 17.724897631069915 22.6040646117517 29.952646519456376 29.71839123772201 42 21.598588188007135 23.23335091818022 26.764136960836034 28.40392393297661 43 21.915115431959205 24.626322003667696 27.737583842037832 25.720978722335268 44 19.20579295098852 28.57726028085513 30.147335895696735 22.069610872459617 45 16.34071896902555 36.9181299771397 24.91395985630668 21.827191197528073 46 21.368729117994324 31.261461551988347 27.178008892908284 20.191800437109052 47 21.303413972416912 25.14381892631949 29.470319290577034 24.082447810686563 48 22.163187379104176 21.952797246715402 33.73966890245434 22.144346471726077 49 20.89205416132841 22.452081292235036 34.519054437661715 22.13681010877484 50 18.731002085060418 31.109478232471677 29.027557967191697 21.13196171527621 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 67.0 1 117.0 2 167.0 3 1878.0 4 3589.0 5 2632.0 6 1675.0 7 1804.0 8 1933.0 9 2046.5 10 2160.0 11 2162.0 12 2164.0 13 2071.0 14 1978.0 15 1757.0 16 1536.0 17 1466.0 18 1396.0 19 1257.0 20 1118.0 21 1032.0 22 946.0 23 904.0 24 862.0 25 862.5 26 863.0 27 942.5 28 1022.0 29 1257.0 30 1492.0 31 1991.5 32 2491.0 33 2512.5 34 2534.0 35 2927.0 36 3320.0 37 3692.0 38 4064.0 39 5364.0 40 6664.0 41 9541.5 42 12419.0 43 14802.0 44 17185.0 45 16791.0 46 16397.0 47 14986.5 48 13576.0 49 12581.5 50 11587.0 51 11050.0 52 10513.0 53 9265.0 54 8017.0 55 7010.0 56 6003.0 57 5345.0 58 4687.0 59 4339.5 60 3992.0 61 3658.0 62 3324.0 63 3080.0 64 2836.0 65 2508.0 66 2180.0 67 1883.5 68 1587.0 69 1319.5 70 1052.0 71 855.0 72 658.0 73 585.0 74 512.0 75 422.0 76 332.0 77 231.5 78 131.0 79 117.5 80 104.0 81 69.5 82 35.0 83 30.5 84 26.0 85 21.5 86 17.0 87 11.5 88 6.0 89 7.0 90 8.0 91 5.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 159228.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.63516466953049 #Duplication Level Percentage of deduplicated Percentage of total 1 72.57713189635554 28.040294420579293 2 8.621866770701258 6.662144848895924 3 3.9776975844468287 4.610370035420906 4 2.509834519977893 3.8787147989047153 5 1.8092265678338049 3.4949883186374255 6 1.3995903637959621 3.2444042505087047 7 1.2012744237458957 3.2488004622302613 8 1.030592672063461 3.18536940739066 9 0.8826684872720179 3.0691838118923807 >10 5.9641080659319226 38.49762604567036 >50 0.022757566890991256 0.5275454065867812 >100 0.0016255404922136609 0.10802120230110283 >500 0.0 0.0 >1k 0.0016255404922136609 1.4325369909814856 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2281 1.4325369909814856 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 172 0.10802120230110283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03328560303464215 0.0 2 0.0 0.0 0.0 0.09546059738236994 0.0 3 0.0 0.0 0.0 0.12560604918732887 0.0 4 0.0 0.0 0.0 0.2110181626347125 0.0 5 0.0 0.0 0.0 0.35609314944607734 0.0 6 0.0 0.0 0.0 0.5576908583917402 0.0 7 0.0 0.0 0.0 0.6971135729896751 0.0 8 0.0 0.0 0.0 1.0852362649785214 0.0 9 0.0 0.0 0.0 1.260456703594845 0.0 10 0.0 0.0 0.0 1.5619112216444344 0.0 11 0.0 0.0 0.0 1.7452960534579345 0.0 12 0.0 0.0 0.0 1.9393573994523576 0.0 13 0.0 0.0 0.0 2.007812696259452 0.0 14 0.0 0.0 0.0 2.0423543597859672 0.0 15 0.0 0.0 0.0 2.075011932574673 0.0 16 0.0 0.0 0.0 2.1516316225789436 0.0 17 0.0 0.0 0.0 2.2602808551259828 0.0 18 0.0 0.0 0.0 2.419172507347954 0.0 19 0.0 0.0 0.0 2.478835380711935 0.0 20 0.0 0.0 0.0 2.5504308287487127 0.0 21 0.0 0.0 0.0 2.6283065792448563 0.0 22 0.0 0.0 0.0 2.7024141482653805 0.0 23 0.0 0.0 0.0 2.792850503680257 0.0 24 0.0 0.0 0.0 2.8738664054060843 0.0 25 0.0 0.0 0.0 2.9197126133594593 0.0 26 0.0 0.0 0.0 2.978747456477504 0.0 27 0.0 0.0 0.0 3.0302459366443086 0.0 28 0.0 0.0 0.0 3.0748360841058107 0.0 29 0.0 0.0 0.0 3.1414072901750947 0.0 30 0.0 0.0 0.0 3.2305875850980983 0.0 31 0.0 0.0 0.0 3.3185118195292285 0.0 32 0.0 0.0 0.0 3.382570904614766 0.0 33 0.0 0.0 0.0 3.441605747732811 0.0 34 0.0 0.0 0.0 3.5106890747858417 0.0 35 0.0 0.0 0.0 3.611173914135705 0.0 36 0.0 0.0 0.0 3.6852814831562286 0.0 37 0.0 0.0 0.0 3.7669254151279925 0.0 38 0.0 0.0 0.0 3.84040495390258 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGGG 30 2.5139452E-6 44.0 3 TCGCCCA 20 7.836038E-4 44.0 18 CTTAACG 25 4.4255263E-5 44.0 1 CTGCTAG 20 7.836038E-4 44.0 1 CTAGAAA 20 7.836038E-4 44.0 41 TCGTTTC 30 2.5139452E-6 44.0 41 TCCGCAA 30 2.5139452E-6 44.0 32 AGTTTAG 20 7.836038E-4 44.0 1 ATAGGGC 25 4.4255263E-5 44.0 4 CATGCAT 20 7.836038E-4 44.0 25 GGCACTA 20 7.836038E-4 44.0 37 ACACCAT 20 7.836038E-4 44.0 23 TGTGGGC 20 7.836038E-4 44.0 4 ATAGCGG 35 1.435601E-7 44.0 2 TAAGTTC 30 2.5139452E-6 44.0 22 GTTAGAG 20 7.836038E-4 44.0 1 CACTGGG 40 8.234565E-9 44.0 3 TACGGGC 20 7.836038E-4 44.0 4 TACGGGA 25 4.4255263E-5 44.0 4 TGCGGGA 30 2.5139452E-6 44.0 4 >>END_MODULE