FastQCFastQC Report
Thu 26 May 2016
SRR1545970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545970_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences136467
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11030.8082540101269905No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT2190.16047835740508692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATT600.044.0000049
CGTAGGG600.044.0000043
CAGTGTC302.5112877E-644.00000444
TAAGGCG302.5112877E-644.0000044
CGATGGT302.5112877E-644.00000410
GCGTAGG302.5112877E-644.0000042
TTAGGCG351.4335819E-744.04
AGTTTAG207.8320864E-444.01
ATCTAGG207.8320864E-444.02
AGATAAT207.8320864E-444.019
CGACGGG454.7293724E-1044.03
GCGGTCT207.8320864E-444.036
TTAGCGG551.8189894E-1244.02
TCCGAGT207.8320864E-444.018
TGGACAG207.8320864E-444.01
ACCTGGG207.8320864E-444.02
GTTAGGC207.8320864E-444.03
TCCTCAA700.044.021
TAACGGG254.4221808E-544.03
ACGGGAT351.4335819E-744.05
Produced by FastQC (version 0.11.5)