FastQCFastQC Report
Thu 26 May 2016
SRR1545969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545969_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences448756
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92462.060362424123577No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4720.10517965219406537No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG207.8513444E-444.0000041
CTATGAC207.8513444E-444.00000440
GATGTAC207.8513444E-444.0000049
CCGGCTA207.8513444E-444.00000422
TACGGGA800.044.0000044
CTAGTAA207.8513444E-444.00000415
ATATACG207.8513444E-444.0000041
GCGATCG207.8513444E-444.0000049
CGTCGGG207.8513444E-444.0000043
TTCGGCA207.8513444E-444.00000418
GCTAACG207.8513444E-444.0000041
CGATAGT408.292773E-944.00000410
CGACGGT700.044.028
AGGCGAT254.438489E-544.06
ACGGGAA454.783942E-1044.05
TTGAGCG302.5242553E-644.01
CGTAGTA254.438489E-544.029
CGGTCTA700.044.031
ACGTAAG254.438489E-544.01
TCGACGG254.438489E-544.02
Produced by FastQC (version 0.11.5)