##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545969_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	448756
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.07026089901862	31.0	28.0	33.0	25.0	33.0
2	28.838442271523945	31.0	28.0	33.0	16.0	33.0
3	28.207636221019886	31.0	27.0	33.0	16.0	34.0
4	32.09513856082147	35.0	32.0	35.0	25.0	37.0
5	33.62096774193548	35.0	33.0	35.0	28.0	37.0
6	33.469956947650836	35.0	33.0	37.0	28.0	37.0
7	34.32561347369172	35.0	35.0	37.0	31.0	37.0
8	34.76664824537165	35.0	35.0	37.0	32.0	37.0
9	36.54161058570805	39.0	37.0	39.0	32.0	39.0
10	36.34737140004813	38.0	35.0	39.0	32.0	39.0
11	36.350201445774545	38.0	35.0	39.0	32.0	39.0
12	35.07839003823904	37.0	34.0	39.0	29.0	39.0
13	35.027732219736336	37.0	34.0	39.0	27.0	39.0
14	35.69236065924467	38.0	33.0	40.0	27.0	41.0
15	36.36284974462737	38.0	35.0	40.0	31.0	41.0
16	36.058564119476955	37.0	34.0	40.0	30.0	41.0
17	35.651679309023166	37.0	34.0	40.0	29.0	41.0
18	35.29311251548726	37.0	33.0	39.0	29.0	40.0
19	34.733977930100096	36.0	33.0	39.0	27.0	40.0
20	35.115755555357474	35.0	34.0	39.0	29.0	40.0
21	35.31347770280509	35.0	34.0	39.0	30.0	40.0
22	35.677631051172575	36.0	34.0	39.0	31.0	40.0
23	36.00712191034771	36.0	35.0	40.0	31.0	41.0
24	35.712173207711984	35.0	34.0	40.0	31.0	41.0
25	35.331110893224825	35.0	34.0	39.0	30.0	41.0
26	35.43625043453458	35.0	34.0	40.0	30.0	41.0
27	35.71696423000473	36.0	34.0	40.0	31.0	41.0
28	35.6551021044844	36.0	35.0	40.0	31.0	41.0
29	35.81132954211197	36.0	35.0	40.0	31.0	41.0
30	35.44451327670271	36.0	34.0	40.0	30.0	41.0
31	34.76307391990302	35.0	34.0	39.0	29.0	41.0
32	34.64934842096819	35.0	34.0	40.0	27.0	41.0
33	34.29748014511227	35.0	34.0	40.0	24.0	41.0
34	33.913400600771915	35.0	34.0	40.0	21.0	41.0
35	33.57663852962412	35.0	33.0	40.0	18.0	41.0
36	33.12457549314104	35.0	33.0	40.0	17.0	41.0
37	33.00880211072387	35.0	33.0	40.0	15.0	41.0
38	32.84210350390858	35.0	33.0	40.0	15.0	41.0
39	32.67704498658514	35.0	32.0	39.0	15.0	40.0
40	32.464285714285715	35.0	32.0	39.0	15.0	40.0
41	32.16690807476669	35.0	31.0	39.0	12.0	40.0
42	32.3153116615711	35.0	32.0	39.0	12.0	40.0
43	32.112551141377494	35.0	31.0	39.0	10.0	40.0
44	31.825123229550133	35.0	31.0	39.0	10.0	40.0
45	31.79844726310066	35.0	31.0	39.0	10.0	40.0
46	31.665470768078865	35.0	31.0	38.0	10.0	40.0
47	31.629725285010117	35.0	31.0	38.0	10.0	40.0
48	31.656372282487588	35.0	31.0	38.0	10.0	40.0
49	31.65194002977119	35.0	31.0	38.0	10.0	40.0
50	31.76871172753122	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	14.0
14	20.0
15	51.0
16	129.0
17	299.0
18	536.0
19	931.0
20	1489.0
21	2152.0
22	3185.0
23	4322.0
24	6141.0
25	9391.0
26	13327.0
27	15151.0
28	14357.0
29	13867.0
30	15106.0
31	18265.0
32	24197.0
33	37001.0
34	57982.0
35	41490.0
36	40509.0
37	55866.0
38	60164.0
39	12807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.83173038354919	21.586118068616354	27.738682045476832	23.843469502357628
2	38.37764843255578	19.92931570831365	29.034709285224043	12.658326573906534
3	27.803082298621078	18.983144515059408	40.37249641230424	12.84127677401528
4	24.28223800907397	24.589309112301564	37.80250291918102	13.32594995944344
5	20.241957767695585	29.23927479521165	37.65743522092183	12.861332216170926
6	18.1091729135655	35.79406180641596	35.59796414978295	10.498801130235584
7	75.41893590280687	4.837372647942312	17.450240219629375	2.2934512296214424
8	76.89635347493962	3.4994518179144127	16.929912914813393	2.674281792332582
9	73.31779407963349	4.509131911328205	18.372790558789188	3.8002834502491334
10	46.35726318979579	20.867241886459457	22.905320485965646	9.870174437779104
11	40.594888981985754	18.5918405547781	29.075934360766208	11.73733610246994
12	36.25957090267317	19.244310939575183	31.638351353519507	12.857766804232144
13	20.792145397498864	33.138498426761984	32.89917015037125	13.170186025367906
14	14.636238846945778	34.80421431691164	36.803296223337405	13.756250612805177
15	14.033461391045469	19.894107265418178	52.04387239390671	14.028558949629645
16	16.689024770699447	16.943951724322346	48.9972278922176	17.369795612760612
17	16.719553610425265	17.028630257868418	36.736444749485244	29.515371382221073
18	21.25698597901755	20.008423285705373	40.42731462086301	18.30727611441407
19	26.39318471507902	21.630685717851126	33.539830108121116	18.43629945894874
20	26.931116241342735	22.006613839146443	33.07632655607947	17.985943363431353
21	20.58111757837221	25.3351487222455	35.700469743022936	18.383263956359357
22	20.520951251905267	21.2224460508606	34.47530506555901	23.78129763167512
23	17.5583167690237	26.012131314121707	33.731916676322996	22.697635240531604
24	18.39150897146779	21.25520327304816	43.89133515763577	16.461952597848274
25	16.725793081318134	22.701200652470384	41.07844797618305	19.494558290028436
26	15.597563040939843	28.87671696868677	35.384485109948386	20.141234880424996
27	16.17359990729929	29.09710399415272	36.276729447628554	18.45256665091943
28	14.839021650963998	24.877438964604373	42.93513624330371	17.348403141127918
29	15.773605255417197	22.006168162654095	42.23564698856394	19.984579593364767
30	18.743147723930154	26.222044942017487	37.66857713323053	17.36623020082183
31	26.832398898287714	22.85696458654592	32.773266541283014	17.537369973883358
32	27.227936785246325	22.89328722067226	33.3216714651169	16.557104528964516
33	26.203772205831232	24.361791262958043	31.304093984258706	18.13034254695202
34	19.400743388389234	24.50396206401697	34.17491910971664	21.920375437877155
35	19.743468611004644	25.386178680619313	34.628172102434284	20.24218060594176
36	27.650883776484324	24.179732415833993	30.072689835901915	18.096693971779764
37	19.83015268876628	30.679924056725703	30.65206927595397	18.837853978554048
38	21.329854085516406	30.3414327607876	27.772330620649083	20.556382533046914
39	21.510130226671066	28.275945057002023	28.995935430389792	21.217989285937126
40	24.098173617734357	24.516441005802708	28.734991844120188	22.650393532342743
41	18.691003574325467	22.766715096845502	30.090516895595826	28.45176443323321
42	21.61530987886513	23.29551025501609	28.476945155050853	26.612234711067927
43	21.616201231849825	24.124245692536704	29.452530996800043	24.807022078813432
44	19.828369982796886	27.32219736337787	30.510344151387393	22.33908850243785
45	17.63853853764629	32.832987191257615	27.21055540204476	22.317918869051333
46	21.509238873686368	28.63694301580369	29.30166950414033	20.55214860636961
47	21.0749271318935	25.622387221563613	30.40783855814741	22.894847088395476
48	21.857089375963774	23.773052616566687	32.912763283387854	21.45709472408168
49	21.25765449375607	22.795015554109582	33.63030243606771	22.317027516066638
50	18.866377274064302	29.042954300332475	30.396919484084894	21.693748941518333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	328.0
1	466.0
2	604.0
3	7273.5
4	13943.0
5	9739.0
6	5535.0
7	5634.5
8	5734.0
9	5952.0
10	6170.0
11	6151.5
12	6133.0
13	5901.5
14	5670.0
15	5280.0
16	4890.0
17	4544.5
18	4199.0
19	3900.0
20	3601.0
21	3405.0
22	3209.0
23	3117.0
24	3025.0
25	2905.5
26	2786.0
27	3056.5
28	3327.0
29	3881.5
30	4436.0
31	5039.0
32	5642.0
33	6635.0
34	7628.0
35	8810.5
36	9993.0
37	10791.0
38	11589.0
39	15327.5
40	19066.0
41	25632.0
42	32198.0
43	37176.0
44	42154.0
45	42481.0
46	42808.0
47	40015.5
48	37223.0
49	34353.5
50	31484.0
51	29109.0
52	26734.0
53	24226.5
54	21719.0
55	19773.5
56	17828.0
57	17118.5
58	16409.0
59	15057.0
60	13705.0
61	12358.5
62	11012.0
63	9885.5
64	8759.0
65	7487.0
66	6215.0
67	5315.5
68	4416.0
69	3772.0
70	3128.0
71	2710.5
72	2293.0
73	1803.5
74	1314.0
75	1077.5
76	841.0
77	644.0
78	447.0
79	355.0
80	263.0
81	187.5
82	112.0
83	95.0
84	78.0
85	61.0
86	44.0
87	33.0
88	22.0
89	21.0
90	20.0
91	16.0
92	12.0
93	10.0
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	448756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.48863846418194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43171106215395	27.52400433681569
2	8.199489699119908	5.983764304439406
3	3.770026339042419	4.1268938425728665
4	2.255477037777194	3.291971447828643
5	1.508962165468973	2.7529987455963223
6	1.096357227338887	2.4002749497576947
7	0.8812260155180779	2.250831623982951
8	0.718974443219087	2.0987518818886217
9	0.5482216750997935	1.800347625085012
>10	5.345492046972399	39.42331898781996
>50	0.22204163069932686	5.037076128916073
>100	0.021408972656959895	1.2461004975070202
>500	0.0	0.0
>1k	0.0	0.0
>5k	6.116849330559969E-4	2.0636656277897023
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9246	2.060362424123577	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	472	0.10517965219406537	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.456764923477346E-4	0.0	0.0	0.045459002219468934	0.0
2	4.456764923477346E-4	0.0	0.0	0.1860699355551792	0.0
3	4.456764923477346E-4	0.0	0.0	0.24980167396090525	0.0
4	4.456764923477346E-4	0.0	0.0	0.3821675921881824	0.0
5	4.456764923477346E-4	0.0	0.0	0.7141965789872448	0.0
6	4.456764923477346E-4	0.0	0.0	1.0275071531077022	0.0
7	4.456764923477346E-4	0.0	0.0	1.2588132526361764	0.0
8	4.456764923477346E-4	0.0	0.0	1.8386383691805792	0.0
9	4.456764923477346E-4	0.0	0.0	2.155960031732166	0.0
10	8.913529846954692E-4	0.0	0.0	2.678738557256059	0.0
11	8.913529846954692E-4	0.0	0.0	3.015001470732425	0.0
12	8.913529846954692E-4	0.0	0.0	3.3777821355034807	0.0
13	0.0011141912308693365	0.0	0.0	3.522849833762668	0.0
14	0.001337029477043204	0.0	0.0	3.5790050717984827	0.0
15	0.001337029477043204	0.0	0.0	3.648753442850903	0.0
16	0.001337029477043204	0.0	0.0	3.780228008093485	0.0
17	0.001337029477043204	0.0	0.0	3.9395573541078	0.0
18	0.001337029477043204	0.0	0.0	4.189359028068705	0.0
19	0.001337029477043204	0.0	0.0	4.298772606940075	0.0
20	0.001337029477043204	0.0	0.0	4.427350274982396	0.0
21	0.0015598677232170712	0.0	0.0	4.577320414657408	0.0
22	0.0015598677232170712	0.0	0.0	4.725285010116856	0.0
23	0.0015598677232170712	0.0	0.0	4.869907031883696	0.0
24	0.0015598677232170712	0.0	0.0	4.979543449001239	0.0
25	0.0015598677232170712	0.0	0.0	5.0682330709784384	0.0
26	0.0015598677232170712	0.0	0.0	5.158928237171202	0.0
27	0.0015598677232170712	0.0	0.0	5.280152243089786	0.0
28	0.0015598677232170712	0.0	0.0	5.379538100883331	0.0
29	0.0015598677232170712	0.0	0.0	5.501430621540436	0.0
30	0.0015598677232170712	0.0	0.0	5.662765511770316	0.0
31	0.0015598677232170712	0.0	0.0	5.794017238766724	0.0
32	0.0017827059693909385	0.0	0.0	5.903653655884267	0.0
33	0.0017827059693909385	0.0	0.0	6.015741293709722	0.0
34	0.0017827059693909385	0.0	0.0	6.149221403167869	0.0
35	0.0017827059693909385	0.0	0.0	6.2956261309040995	0.0
36	0.0017827059693909385	0.0	0.0	6.428660563869898	0.0
37	0.0020055442155648058	0.0	0.0	6.568157305974739	0.0
38	0.0020055442155648058	0.0	0.0	6.744199520452094	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.8513444E-4	44.000004	1
CTATGAC	20	7.8513444E-4	44.000004	40
GATGTAC	20	7.8513444E-4	44.000004	9
CCGGCTA	20	7.8513444E-4	44.000004	22
TACGGGA	80	0.0	44.000004	4
CTAGTAA	20	7.8513444E-4	44.000004	15
ATATACG	20	7.8513444E-4	44.000004	1
GCGATCG	20	7.8513444E-4	44.000004	9
CGTCGGG	20	7.8513444E-4	44.000004	3
TTCGGCA	20	7.8513444E-4	44.000004	18
GCTAACG	20	7.8513444E-4	44.000004	1
CGATAGT	40	8.292773E-9	44.000004	10
CGACGGT	70	0.0	44.0	28
AGGCGAT	25	4.438489E-5	44.0	6
ACGGGAA	45	4.783942E-10	44.0	5
TTGAGCG	30	2.5242553E-6	44.0	1
CGTAGTA	25	4.438489E-5	44.0	29
CGGTCTA	70	0.0	44.0	31
ACGTAAG	25	4.438489E-5	44.0	1
TCGACGG	25	4.438489E-5	44.0	2
>>END_MODULE