FastQCFastQC Report
Thu 26 May 2016
SRR1545968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545968_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences448730
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96172.1431595837140374No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5220.11632830432554096No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC207.851347E-444.026
TTAATCG207.851347E-444.020
TACTTAG254.4384877E-544.01
ACGTTAG351.4434409E-744.01
TACGGGA454.783942E-1044.04
CGGATGG207.851347E-444.02
CACTAGT254.4384877E-544.036
TGTTACG207.851347E-444.01
CGATGGT207.851347E-444.010
CGATGAT207.851347E-444.010
CAATCGT207.851347E-444.017
ATCATAC207.851347E-444.018
GATTAGT207.851347E-444.043
GGCCTAT207.851347E-444.08
TATCCGG254.4384877E-544.02
GCATATC207.851347E-444.028
CGTTTTT61650.043.107871
ATGGGCG1050.041.904765
CGACCCG950.041.6842132
ACGGGAT900.041.5555575
Produced by FastQC (version 0.11.5)