##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545968_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	448730
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.0476723196577	31.0	28.0	33.0	25.0	33.0
2	28.830234216566755	31.0	28.0	33.0	16.0	33.0
3	28.234936375994472	31.0	27.0	33.0	16.0	34.0
4	32.09935596015421	35.0	32.0	35.0	25.0	37.0
5	33.65919595302297	35.0	33.0	35.0	28.0	37.0
6	33.558939674191606	35.0	33.0	37.0	28.0	37.0
7	34.394410892964586	35.0	35.0	37.0	31.0	37.0
8	34.81423350344305	35.0	35.0	37.0	32.0	37.0
9	36.674320861096874	39.0	37.0	39.0	32.0	39.0
10	36.35842934504045	39.0	35.0	39.0	32.0	39.0
11	36.410703541104894	39.0	35.0	39.0	32.0	39.0
12	35.0372807701736	37.0	34.0	39.0	29.0	39.0
13	35.05947451697011	37.0	34.0	39.0	27.0	39.0
14	35.72984868406392	38.0	33.0	40.0	27.0	41.0
15	36.422173690192324	38.0	35.0	40.0	31.0	41.0
16	36.115940543311126	37.0	34.0	40.0	31.0	41.0
17	35.713373297974286	37.0	34.0	40.0	30.0	41.0
18	35.38538542107726	37.0	34.0	39.0	29.0	40.0
19	34.83185211597174	37.0	33.0	39.0	27.0	40.0
20	35.102841352260825	35.0	34.0	39.0	29.0	40.0
21	35.241875961045615	35.0	34.0	39.0	30.0	40.0
22	35.656515053595705	36.0	34.0	39.0	31.0	40.0
23	36.040295946337444	36.0	35.0	40.0	31.0	41.0
24	35.62765360016045	35.0	34.0	40.0	31.0	41.0
25	35.25680476010073	35.0	34.0	39.0	30.0	41.0
26	35.41319947407127	35.0	34.0	39.0	30.0	41.0
27	35.74537026719854	36.0	34.0	40.0	31.0	41.0
28	35.684371448309676	36.0	35.0	40.0	31.0	41.0
29	35.83190114322644	36.0	35.0	40.0	31.0	41.0
30	35.50035210482918	36.0	35.0	40.0	30.0	41.0
31	34.81105341742251	35.0	34.0	39.0	29.0	41.0
32	34.73478929423038	35.0	34.0	40.0	27.0	41.0
33	34.39906625364919	35.0	34.0	40.0	24.0	41.0
34	33.94451229024135	35.0	34.0	40.0	21.0	41.0
35	33.63550687495822	35.0	33.0	40.0	18.0	41.0
36	33.17418492189067	35.0	33.0	40.0	17.0	41.0
37	33.05249704722216	35.0	33.0	40.0	15.0	41.0
38	32.920745214271385	35.0	33.0	40.0	15.0	41.0
39	32.72770262741515	35.0	33.0	39.0	15.0	40.0
40	32.476174982729034	35.0	32.0	39.0	15.0	40.0
41	32.171983152452476	35.0	31.0	39.0	12.0	40.0
42	32.33290620194772	35.0	32.0	39.0	12.0	40.0
43	32.148978227441894	35.0	32.0	39.0	12.0	40.0
44	31.85797027165556	35.0	31.0	39.0	10.0	40.0
45	31.827234639983956	35.0	31.0	39.0	10.0	40.0
46	31.683551356049296	35.0	31.0	38.0	10.0	40.0
47	31.64734918547902	35.0	31.0	38.0	10.0	40.0
48	31.6719809239409	35.0	31.0	38.0	10.0	40.0
49	31.738268000802265	35.0	31.0	38.0	10.0	40.0
50	31.885195997593208	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	7.0
14	15.0
15	45.0
16	100.0
17	264.0
18	543.0
19	899.0
20	1395.0
21	2226.0
22	3019.0
23	4196.0
24	6090.0
25	9130.0
26	13080.0
27	15138.0
28	14128.0
29	13783.0
30	14899.0
31	18060.0
32	23934.0
33	37044.0
34	59154.0
35	41272.0
36	41011.0
37	56974.0
38	60647.0
39	11672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.975018385220512	21.485971519622044	27.569139571680072	23.96987052347737
2	38.41129409667283	19.826844650457957	28.949033940231317	12.81282731263789
3	27.8254184030486	18.825351547701292	40.44837652931607	12.900853519934035
4	24.11516947830544	24.544380808058296	37.959129097675664	13.3813206159606
5	20.21839413455753	29.127983419873864	37.80335613843514	12.850266307133465
6	18.189780045907337	35.84716867604128	35.55991353375081	10.403137744300581
7	75.40191206293316	4.72399884117398	17.565351101998974	2.308737993893878
8	76.86515276446862	3.414748289617365	17.04699930916141	2.673099636752613
9	73.35123570966951	4.510061729770686	18.521382568582446	3.617319991977358
10	46.258551913177186	20.731843201925436	22.872997125220067	10.136607759677311
11	40.41717736723642	18.52762240099837	29.28509348606066	11.770106745704544
12	36.09052214026252	18.932320103402937	31.845876139326545	13.131281617008
13	20.91591825819535	32.925812849597754	33.03768413076906	13.120584761437836
14	14.758317919461591	34.66984600985002	36.8194682771377	13.752367793550688
15	14.056559623827244	19.922002094800884	52.00900318677155	14.012435094600317
16	16.76152697613264	16.785594901165513	49.08073897443897	17.372139148262875
17	16.762864083078913	17.087781071022665	36.7530586321396	29.39629621375883
18	21.162837340939987	19.825284692353975	40.683038798386555	18.32883916831948
19	26.25142067613041	21.66336104116061	33.68150112539834	18.40371715731063
20	26.78470349653466	21.718405277115416	33.327836338109776	18.169054888240144
21	20.60281238161032	25.153878724399974	35.965056938470795	18.27825195551891
22	20.422525795021503	21.12673545339068	34.61725313663004	23.83348561495777
23	17.316426358834935	25.958817106054866	33.952710984333564	22.77204555077664
24	18.388786129743945	21.16996857798676	43.98457869988634	16.45666659238295
25	16.839079179016338	22.43375749337018	41.20607046553607	19.52109286207742
26	15.538519822610478	28.711920308426002	35.47188732645466	20.27767254250886
27	16.093864907628195	28.943685512446237	36.42479887683017	18.5376507030954
28	15.002117085998263	24.637309740824104	43.13395583090054	17.22661734227709
29	15.861654001292536	21.81356272145834	42.376261894680546	19.948521382568583
30	18.67136139772246	26.15849174336461	37.7931049851804	17.377041873732534
31	26.80030307757449	22.895505092148955	32.90040781761861	17.403784012657947
32	27.177144385265084	22.716109910190983	33.532859403204604	16.573886301339336
33	26.095202014574465	24.239966126624026	31.491765649722552	18.173066209078957
34	19.39339914870858	24.364539923784903	34.3077128785684	21.934348048938116
35	19.694916765092596	25.378066989058006	34.762106389142694	20.164909856706707
36	27.53214627949992	24.048982684465045	30.181400842377375	18.237470193657657
37	19.828181757404227	30.595681144563546	30.752122657277205	18.82401444075502
38	21.376997303500993	30.22975954360083	27.92481001938805	20.46843313351013
39	21.523633365275334	28.12582176364406	29.10547545294498	21.245069418135625
40	24.157734049428388	24.44097787088004	28.901566643638716	22.499721436052862
41	18.727297038308112	22.658168609185925	30.2803467564014	28.33418759610456
42	21.590043010273437	23.213290843045932	28.663338756044837	26.533327390635797
43	21.628819111715288	24.050988344884452	29.58059412118646	24.739598422213803
44	19.946070019833755	27.15530497180933	30.478238584449446	22.420386423907473
45	17.485347536380452	32.81505582421501	27.507632652151624	22.19196398725291
46	21.692777393978563	28.45029304927239	29.37311969335681	20.483809863392242
47	21.1630601920977	25.62699173222205	30.47489581708377	22.735052258596482
48	21.838299199964347	23.833039912642345	32.95768056515054	21.370980322242776
49	21.26690883159138	22.683350790007353	33.66902146056649	22.380718917834777
50	18.94413121476166	28.96931339558309	30.508546341898246	21.578009047757003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	333.0
1	467.0
2	601.0
3	7565.0
4	14529.0
5	10016.0
6	5503.0
7	5542.0
8	5581.0
9	5927.0
10	6273.0
11	6324.5
12	6376.0
13	5968.0
14	5560.0
15	5171.0
16	4782.0
17	4501.5
18	4221.0
19	3892.0
20	3563.0
21	3369.0
22	3175.0
23	3072.0
24	2969.0
25	2836.5
26	2704.0
27	3044.5
28	3385.0
29	3856.0
30	4327.0
31	4994.5
32	5662.0
33	6706.5
34	7751.0
35	8928.5
36	10106.0
37	10879.0
38	11652.0
39	15159.5
40	18667.0
41	25308.0
42	31949.0
43	36931.0
44	41913.0
45	42133.5
46	42354.0
47	39638.0
48	36922.0
49	34333.5
50	31745.0
51	29243.5
52	26742.0
53	24347.0
54	21952.0
55	19860.5
56	17769.0
57	17137.0
58	16505.0
59	15173.5
60	13842.0
61	12427.0
62	11012.0
63	9919.0
64	8826.0
65	7552.0
66	6278.0
67	5357.5
68	4437.0
69	3793.0
70	3149.0
71	2721.5
72	2294.0
73	1873.0
74	1452.0
75	1158.5
76	865.0
77	650.5
78	436.0
79	352.0
80	268.0
81	199.5
82	131.0
83	102.0
84	73.0
85	57.5
86	42.0
87	31.0
88	20.0
89	18.0
90	16.0
91	13.5
92	11.0
93	8.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	448730.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.10995317213372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88129058927356	27.039598966476053
2	8.47654414543742	6.12175224306539
3	3.823122880386124	4.141583645461678
4	2.294432748936847	3.3140743648287825
5	1.518997382042423	2.7425462167072805
6	1.1282214127489774	2.4444013429298477
7	0.871976346456667	2.2040917526430945
8	0.7183725146163555	2.0752318290355634
9	0.6410116932141422	2.083221200227754
>10	5.391050834715638	39.325822449670454
>50	0.23388626148129854	5.178039940192383
>100	0.019852414767643378	1.0582789944392494
>500	6.203879614888556E-4	0.11693938084193231
>1k	0.0	0.0
>5k	6.203879614888556E-4	2.1544176734805807
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9617	2.1431595837140374	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	522	0.11632830432554096	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04011320838811758	0.0
2	0.0	0.0	0.0	0.16646981481068795	0.0
3	0.0	0.0	0.0	0.2304280970739643	0.0
4	0.0	0.0	0.0	0.370601475274664	0.0
5	0.0	0.0	0.0	0.7071067234194282	0.0
6	0.0	0.0	0.0	1.0213268557930157	0.0
7	0.0	0.0	0.0	1.2372696276157156	0.0
8	0.0	0.0	0.0	1.8213625119782497	0.0
9	0.0	0.0	0.0	2.109286207741849	0.0
10	0.0	0.0	0.0	2.6298665121565308	0.0
11	0.0	0.0	0.0	2.9641432487241772	0.0
12	0.0	0.0	0.0	3.319813696432153	0.0
13	0.0	0.0	0.0	3.459764223475141	0.0
14	0.0	0.0	0.0	3.5125799478528292	0.0
15	0.0	0.0	0.0	3.588126490317117	0.0
16	0.0	0.0	0.0	3.71983152452477	0.0
17	0.0	0.0	0.0	3.8713703117687697	0.0
18	0.0	0.0	0.0	4.123860673456199	0.0
19	0.0	0.0	0.0	4.223475140953357	0.0
20	0.0	0.0	0.0	4.338243487174916	0.0
21	0.0	0.0	0.0	4.467497158647739	0.0
22	0.0	0.0	0.0	4.6085619414792856	0.0
23	0.0	0.0	0.0	4.752078087045662	0.0
24	0.0	0.0	0.0	4.86038374969358	0.0
25	0.0	0.0	0.0	4.951084170882268	0.0
26	0.0	0.0	0.0	5.052481447641121	0.0
27	0.0	0.0	0.0	5.167249793862679	0.0
28	0.0	0.0	0.0	5.271766986829497	0.0
29	0.0	0.0	0.0	5.391660909678426	0.0
30	0.0	0.0	0.0	5.546765315445814	0.0
31	0.0	0.0	0.0	5.674013326499231	0.0
32	2.2285115771176431E-4	0.0	0.0	5.7887816727207895	0.0
33	2.2285115771176431E-4	0.0	0.0	5.891293205268202	0.0
34	4.4570231542352863E-4	0.0	0.0	6.009404318855436	0.0
35	4.4570231542352863E-4	0.0	0.0	6.154480422525795	0.0
36	4.4570231542352863E-4	0.0	0.0	6.281059880106077	0.0
37	4.4570231542352863E-4	0.0	0.0	6.409867849263477	0.0
38	4.4570231542352863E-4	0.0	0.0	6.578120473335859	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	20	7.851347E-4	44.0	26
TTAATCG	20	7.851347E-4	44.0	20
TACTTAG	25	4.4384877E-5	44.0	1
ACGTTAG	35	1.4434409E-7	44.0	1
TACGGGA	45	4.783942E-10	44.0	4
CGGATGG	20	7.851347E-4	44.0	2
CACTAGT	25	4.4384877E-5	44.0	36
TGTTACG	20	7.851347E-4	44.0	1
CGATGGT	20	7.851347E-4	44.0	10
CGATGAT	20	7.851347E-4	44.0	10
CAATCGT	20	7.851347E-4	44.0	17
ATCATAC	20	7.851347E-4	44.0	18
GATTAGT	20	7.851347E-4	44.0	43
GGCCTAT	20	7.851347E-4	44.0	8
TATCCGG	25	4.4384877E-5	44.0	2
GCATATC	20	7.851347E-4	44.0	28
CGTTTTT	6165	0.0	43.10787	1
ATGGGCG	105	0.0	41.90476	5
CGACCCG	95	0.0	41.68421	32
ACGGGAT	90	0.0	41.555557	5
>>END_MODULE