FastQCFastQC Report
Thu 26 May 2016
SRR1545967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545967_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences441634
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94952.1499703374287304No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5200.1177445577106835No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG254.4383734E-544.01
TAGACGG502.7284841E-1144.02
ACGTGTT207.8512105E-444.044
GCGATAC254.4383734E-544.09
CCGACGG302.5241643E-644.02
AGTTACG254.4383734E-544.01
CGGTCTA502.7284841E-1144.031
ACGTAAG207.8512105E-444.01
GACGCCT207.8512105E-444.011
AGTACGG351.4433681E-744.02
CGTTTTT58250.043.1313321
TAACGGG1400.042.4285743
GTAGGGT1400.040.8571434
TACGGGA700.040.8571434
GTAACGG650.040.6153872
CGACGGT557.8216544E-1140.028
TAGGGTA1650.040.05
GCATAGG1000.039.6000022
TAGCGGG1800.039.111113
ATGTACG452.345223E-839.111111
Produced by FastQC (version 0.11.5)