##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545967_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	441634
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.941494540728296	31.0	28.0	33.0	23.0	33.0
2	28.71340748221378	31.0	28.0	33.0	16.0	33.0
3	28.12527794508575	31.0	26.0	33.0	16.0	34.0
4	32.000403048678315	35.0	32.0	35.0	25.0	37.0
5	33.61486660900202	35.0	33.0	35.0	28.0	37.0
6	33.49696807763895	35.0	33.0	37.0	28.0	37.0
7	34.32208570898074	35.0	35.0	37.0	31.0	37.0
8	34.78274770511329	35.0	35.0	37.0	32.0	37.0
9	36.65228447085143	39.0	37.0	39.0	32.0	39.0
10	36.42503521015139	39.0	35.0	39.0	32.0	39.0
11	36.42057450286889	39.0	35.0	39.0	32.0	39.0
12	35.19499630916098	37.0	34.0	39.0	30.0	39.0
13	35.12741319735346	37.0	34.0	39.0	27.0	39.0
14	35.78333416358342	38.0	34.0	40.0	27.0	41.0
15	36.39036396654243	38.0	35.0	40.0	31.0	41.0
16	36.06737705883152	37.0	34.0	40.0	31.0	41.0
17	35.665748108161964	37.0	34.0	40.0	30.0	41.0
18	35.31231064637234	37.0	33.0	39.0	29.0	40.0
19	34.73345802180086	36.0	33.0	39.0	27.0	40.0
20	35.18462346649035	35.0	34.0	39.0	30.0	40.0
21	35.290924158918926	35.0	34.0	39.0	30.0	40.0
22	35.66488087420805	36.0	34.0	39.0	31.0	40.0
23	36.025235828763186	36.0	35.0	40.0	31.0	41.0
24	35.63330948251267	35.0	34.0	40.0	31.0	41.0
25	35.28374400521699	35.0	34.0	39.0	30.0	41.0
26	35.42337999338819	35.0	34.0	40.0	30.0	41.0
27	35.747940602399275	36.0	34.0	40.0	31.0	41.0
28	35.68821920413736	36.0	35.0	40.0	31.0	41.0
29	35.833094825126686	36.0	35.0	40.0	31.0	41.0
30	35.487310759588254	36.0	35.0	40.0	30.0	41.0
31	34.781187589723615	35.0	34.0	39.0	29.0	41.0
32	34.723474641898044	35.0	34.0	40.0	27.0	41.0
33	34.413086401862174	35.0	34.0	40.0	24.0	41.0
34	33.955071393959706	35.0	34.0	40.0	21.0	41.0
35	33.61319101337306	35.0	33.0	40.0	18.0	41.0
36	33.19252593776747	35.0	33.0	40.0	18.0	41.0
37	33.097080387832456	35.0	33.0	40.0	16.0	41.0
38	32.93339280943043	35.0	33.0	40.0	15.0	41.0
39	32.72090237617575	35.0	33.0	40.0	15.0	40.0
40	32.52397686772305	35.0	32.0	39.0	15.0	40.0
41	32.20893998197602	35.0	32.0	39.0	12.0	40.0
42	32.39568285050517	35.0	32.0	39.0	12.0	40.0
43	32.19345430831865	35.0	32.0	39.0	12.0	40.0
44	31.926805907153888	35.0	31.0	39.0	10.0	40.0
45	31.90445934869145	35.0	31.0	39.0	10.0	40.0
46	31.750247942866718	35.0	31.0	38.0	10.0	40.0
47	31.736573723943355	35.0	31.0	38.0	10.0	40.0
48	31.746817500464186	35.0	31.0	38.0	10.0	40.0
49	31.805182571994003	35.0	31.0	38.0	10.0	40.0
50	31.872450943541484	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	8.0
14	14.0
15	51.0
16	122.0
17	270.0
18	546.0
19	881.0
20	1421.0
21	1991.0
22	2992.0
23	4247.0
24	6066.0
25	9135.0
26	13030.0
27	14741.0
28	13972.0
29	13761.0
30	14523.0
31	17436.0
32	23622.0
33	36592.0
34	57456.0
35	40728.0
36	39818.0
37	55419.0
38	59810.0
39	12976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.86432656905945	21.380600225526113	27.69125565513525	24.06381755027919
2	38.445183115430424	19.762291852529472	29.113926916858755	12.67859811518135
3	27.784545573936793	18.850450825796926	40.51794019482195	12.84706340544433
4	24.247680205781258	24.56332619318259	37.83336427901837	13.355629322017778
5	20.15537752980975	29.18344149227641	37.839704370587405	12.82147660732643
6	18.11364161273815	35.7803067698592	35.74928560753928	10.356766009863371
7	75.35447904826168	4.812355932740686	17.50408709474361	2.3290779242540203
8	76.73050535058442	3.5074292287278603	17.046694774405957	2.715370646281763
9	73.23417128210238	4.564186634181245	18.555636567836714	3.646005515879665
10	46.15201728127816	20.956266954084153	22.933469796256627	9.958245968381057
11	40.43370754969047	18.686514172368977	29.21899129143137	11.66078698650919
12	36.080102528337946	19.15251090269318	31.77585964848721	12.991526920481666
13	20.87678937762944	33.04546298518683	32.9288505866849	13.148897050498828
14	14.783282084259817	34.65471408451342	36.7523786664976	13.809625164729164
15	14.019527482032634	19.932115733842956	52.05668041862719	13.991676365497222
16	16.667195007630752	16.80645059030781	49.11125502112609	17.415099380935345
17	16.60152977352287	17.13522962453072	36.83774347083784	29.425497131108564
18	21.11431637962657	19.888414388384952	40.67621605220613	18.321053179782353
19	26.4255016597454	21.614957181738724	33.543386605197966	18.416154553317906
20	26.914141574244738	21.847276251375575	33.162754679214004	18.07582749516568
21	20.474419994837355	25.284058745477022	35.82763102478523	18.413890234900393
22	20.460834084332273	21.215304981047655	34.614409216681686	23.709451717938386
23	17.49185977528904	25.93799390445482	33.96454983085541	22.605596489400725
24	18.44785501116309	21.106844128848774	43.978045168623794	16.467255691364343
25	16.68032805445233	22.59970926151519	41.108021574425884	19.611941109606597
26	15.710520476231448	28.726954899305763	35.41529864095608	20.147225983506704
27	16.156138340798037	29.030147135410772	36.28389118591413	18.529823337877065
28	14.94404869190325	24.793833808085427	42.9896701793793	17.27244732063202
29	15.771883505346057	21.995362675880934	42.42245841579226	19.81029540298075
30	18.79769220666887	26.127970219684173	37.77290697727077	17.301430596376186
31	26.724391690857136	22.809385146976908	32.86295892073527	17.603264241430686
32	27.1668395096392	22.885239813963597	33.47387202978032	16.474048646616883
33	26.05913493979177	24.26036038891933	31.42647531666493	18.254029354623967
34	19.35222378711784	24.363160445074428	34.437565948273914	21.847049819533822
35	19.532010669468384	25.583401640272264	34.79012032588071	20.094467364378648
36	27.68695345014197	24.091895098656355	30.211215621985627	18.009935829216047
37	19.807804652721483	30.580299524040267	30.749896973512	18.861998849726245
38	21.431094526236656	30.26737071873995	27.752392252408104	20.54914250261529
39	21.520082240044925	28.057848806930625	29.09966171082842	21.322407242196025
40	24.03279638795926	24.43561863443485	29.06773482114149	22.463850156464403
41	18.76644461250719	22.661751586155052	30.237028851945276	28.334774949392482
42	21.646204775900408	23.281042673344896	28.621211229207894	26.451541321546802
43	21.66771580086678	24.155748878030224	29.57177210087991	24.60476322022308
44	19.881847864974166	27.246090654252164	30.580299524040267	22.291761956733403
45	17.62975676691559	32.83329634946585	27.4245189455522	22.11242793806636
46	21.46415357513235	28.62370197946716	29.498408184152485	20.413736261248
47	21.177037999791683	25.623706508103993	30.463234261854836	22.73602123024948
48	21.8257652264092	23.90690028394553	32.852090192331204	21.415244297314064
49	21.253345530461875	22.898372860785173	33.76166690064623	22.086614708106715
50	18.786597046423058	29.014976202013433	30.622868710289513	21.575558041273997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	305.0
1	457.5
2	610.0
3	7473.5
4	14337.0
5	9912.5
6	5488.0
7	5557.0
8	5626.0
9	5982.5
10	6339.0
11	6215.0
12	6091.0
13	5865.5
14	5640.0
15	5228.5
16	4817.0
17	4326.5
18	3836.0
19	3637.0
20	3438.0
21	3230.5
22	3023.0
23	2918.5
24	2814.0
25	2845.5
26	2877.0
27	3111.0
28	3345.0
29	3817.0
30	4289.0
31	4893.0
32	5497.0
33	6446.5
34	7396.0
35	8740.0
36	10084.0
37	10843.0
38	11602.0
39	15122.5
40	18643.0
41	25087.0
42	31531.0
43	36596.5
44	41662.0
45	41707.0
46	41752.0
47	39102.5
48	36453.0
49	33715.0
50	30977.0
51	28611.5
52	26246.0
53	23715.0
54	21184.0
55	19474.0
56	17764.0
57	16910.5
58	16057.0
59	14727.0
60	13397.0
61	12179.5
62	10962.0
63	9884.5
64	8807.0
65	7462.0
66	6117.0
67	5153.5
68	4190.0
69	3592.5
70	2995.0
71	2620.5
72	2246.0
73	1811.5
74	1377.0
75	1104.5
76	832.0
77	648.0
78	464.0
79	344.5
80	225.0
81	172.5
82	120.0
83	95.5
84	71.0
85	58.5
86	46.0
87	39.0
88	32.0
89	25.5
90	19.0
91	11.5
92	4.0
93	5.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	441634.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.803110375518294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.65834551671436	26.730009849885057
2	8.322238681166224	5.959240601464042
3	3.920962042333678	4.211479103396709
4	2.32105830553625	3.324044268045113
5	1.5830382978013577	2.83388474524273
6	1.1493563657195063	2.4690319693596026
7	0.9170443572005372	2.298312823806794
8	0.7665918845175809	2.195709908348762
9	0.6169561557730432	1.988005440779795
>10	5.5016568759105	39.6742022010669
>50	0.22050988615826525	4.901915787912353
>100	0.020970680816203898	1.135551714897682
>500	6.35475176248603E-4	0.1183103369558795
>1k	0.0	0.0
>5k	6.35475176248603E-4	2.160301248838607
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9495	2.1499703374287304	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	520	0.1177445577106835	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04143702704049054	0.0
2	0.0	0.0	0.0	0.17661683656602525	0.0
3	0.0	0.0	0.0	0.23865916120588543	0.0
4	0.0	0.0	0.0	0.36749887916238333	0.0
5	0.0	0.0	0.0	0.7030708686378313	0.0
6	0.0	0.0	0.0	1.004678081850582	0.0
7	0.0	0.0	0.0	1.2234112409823519	0.0
8	0.0	0.0	0.0	1.823455621623335	0.0
9	0.0	0.0	0.0	2.100155332243441	0.0
10	0.0	0.0	0.0	2.5937767472613067	0.0
11	0.0	0.0	0.0	2.9080641436121315	0.0
12	2.2643184175131443E-4	0.0	0.0	3.2438625649293304	0.0
13	2.2643184175131443E-4	0.0	0.0	3.3826652839228863	0.0
14	2.2643184175131443E-4	0.0	0.0	3.4361031985761965	0.0
15	2.2643184175131443E-4	0.0	0.0	3.5072027968861095	0.0
16	2.2643184175131443E-4	0.0	0.0	3.638759696943623	0.0
17	2.2643184175131443E-4	0.0	0.0	3.800884895637564	0.0
18	2.2643184175131443E-4	0.0	0.0	4.035921147375428	0.0
19	2.2643184175131443E-4	0.0	0.0	4.131022520910981	0.0
20	2.2643184175131443E-4	0.0	0.0	4.24989923783042	0.0
21	2.2643184175131443E-4	0.0	0.0	4.381229706046183	0.0
22	2.2643184175131443E-4	0.0	0.0	4.5107487195279345	0.0
23	2.2643184175131443E-4	0.0	0.0	4.642984915110702	0.0
24	4.5286368350262886E-4	0.0	0.0	4.747143562316307	0.0
25	6.792955252539433E-4	0.0	0.0	4.834546253232315	0.0
26	6.792955252539433E-4	0.0	0.0	4.928062603875607	0.0
27	6.792955252539433E-4	0.0	0.0	5.040825661067762	0.0
28	6.792955252539433E-4	0.0	0.0	5.130266238559531	0.0
29	6.792955252539433E-4	0.0	0.0	5.238953522600162	0.0
30	6.792955252539433E-4	0.0	0.0	5.3861342197385165	0.0
31	6.792955252539433E-4	0.0	0.0	5.507048823233719	0.0
32	6.792955252539433E-4	0.0	0.0	5.610981038597572	0.0
33	6.792955252539433E-4	0.0	0.0	5.724196959473229	0.0
34	6.792955252539433E-4	0.0	0.0	5.839677198766399	0.0
35	6.792955252539433E-4	0.0	0.0	5.980291372493966	0.0
36	6.792955252539433E-4	0.0	0.0	6.0882993610093425	0.0
37	6.792955252539433E-4	0.0	0.0	6.2053646231947726	0.0
38	6.792955252539433E-4	0.0	0.0	6.356847525326401	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	4.4383734E-5	44.0	1
TAGACGG	50	2.7284841E-11	44.0	2
ACGTGTT	20	7.8512105E-4	44.0	44
GCGATAC	25	4.4383734E-5	44.0	9
CCGACGG	30	2.5241643E-6	44.0	2
AGTTACG	25	4.4383734E-5	44.0	1
CGGTCTA	50	2.7284841E-11	44.0	31
ACGTAAG	20	7.8512105E-4	44.0	1
GACGCCT	20	7.8512105E-4	44.0	11
AGTACGG	35	1.4433681E-7	44.0	2
CGTTTTT	5825	0.0	43.131332	1
TAACGGG	140	0.0	42.428574	3
GTAGGGT	140	0.0	40.857143	4
TACGGGA	70	0.0	40.857143	4
GTAACGG	65	0.0	40.615387	2
CGACGGT	55	7.8216544E-11	40.0	28
TAGGGTA	165	0.0	40.0	5
GCATAGG	100	0.0	39.600002	2
TAGCGGG	180	0.0	39.11111	3
ATGTACG	45	2.345223E-8	39.11111	1
>>END_MODULE