FastQCFastQC Report
Thu 26 May 2016
SRR1545966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545966_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences385132
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50181.3029299045522054No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT9530.24744762834560619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATC207.849954E-444.00000422
ACGTTAG207.849954E-444.0000041
CCTAACG207.849954E-444.0000041
GCGTTAG207.849954E-444.0000041
TCGTGAT207.849954E-444.00000428
GGAACCT207.849954E-444.0000048
GGTGCGA207.849954E-444.00000441
AGTACGG207.849954E-444.0000042
ACGACGG408.287316E-944.00000427
TCACGAC351.4427314E-744.025
CTCACGA351.4427314E-744.024
CGTGCGG454.783942E-1044.02
AGGCGGT351.4427314E-744.06
CGACAGG254.4373097E-544.02
ATCGTAG351.4427314E-744.01
ATCCGGT254.4373097E-544.026
GTCCGGT254.4373097E-544.026
ATAGTCT254.4373097E-544.024
TACCGGG551.8189894E-1244.03
TAATCGT254.4373097E-544.021
Produced by FastQC (version 0.11.5)