##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545966_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	385132
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.938140689425964	28.0	25.0	30.0	16.0	33.0
2	26.13334648899598	30.0	25.0	31.0	10.0	33.0
3	26.364649003458556	30.0	25.0	31.0	16.0	33.0
4	27.92764039342355	32.0	19.0	35.0	10.0	35.0
5	31.179123001983736	33.0	30.0	35.0	19.0	37.0
6	29.879166104088988	33.0	28.0	35.0	17.0	37.0
7	29.84574898995669	32.0	28.0	35.0	17.0	35.0
8	32.40739538651683	33.0	32.0	35.0	28.0	35.0
9	27.095769762055607	30.0	23.0	34.0	10.0	35.0
10	31.68822377782163	34.0	30.0	37.0	17.0	37.0
11	33.73259298110778	35.0	32.0	37.0	27.0	39.0
12	33.08119553815315	35.0	31.0	37.0	23.0	39.0
13	32.94528629145332	35.0	31.0	38.0	22.0	39.0
14	32.68630755169656	35.0	30.0	38.0	21.0	40.0
15	31.747432049271417	34.0	27.0	38.0	18.0	40.0
16	30.426090275541892	33.0	26.0	38.0	17.0	40.0
17	30.549634930361538	32.0	26.0	37.0	17.0	40.0
18	31.49623246055898	34.0	28.0	38.0	18.0	39.0
19	31.17486212519344	34.0	27.0	37.0	18.0	39.0
20	31.838494334410022	34.0	30.0	37.0	21.0	39.0
21	32.61029205571077	34.0	31.0	37.0	23.0	39.0
22	32.985516654030306	34.0	31.0	37.0	25.0	39.0
23	34.28920474019297	35.0	33.0	38.0	29.0	40.0
24	33.98123760165346	35.0	33.0	38.0	27.0	40.0
25	32.72680275853474	35.0	31.0	37.0	23.0	39.0
26	26.43689436349096	33.0	21.0	36.0	0.0	39.0
27	29.65762128309255	34.0	24.0	37.0	15.0	39.0
28	31.1794190043933	34.0	27.0	36.0	20.0	39.0
29	32.510915737980746	34.0	30.0	37.0	23.0	39.0
30	32.23516352834872	34.0	30.0	37.0	22.0	39.0
31	31.162803921772277	34.0	29.0	36.0	20.0	38.0
32	31.375676391470975	34.0	30.0	37.0	19.0	39.0
33	31.128023638648568	34.0	29.0	37.0	18.0	39.0
34	31.041326090794843	34.0	30.0	37.0	15.0	39.0
35	30.21440441199381	34.0	28.0	37.0	10.0	39.0
36	29.884247478786495	34.0	27.0	37.0	10.0	39.0
37	29.930766594310523	34.0	27.0	37.0	10.0	39.0
38	29.654588556650708	33.0	27.0	36.0	10.0	38.0
39	29.583942648234892	33.0	26.0	36.0	10.0	39.0
40	29.47093983361549	33.0	26.0	36.0	10.0	38.0
41	28.909760809280975	33.0	24.0	36.0	9.0	38.0
42	29.066847210826417	33.0	25.0	36.0	9.0	38.0
43	28.62420936198498	33.0	24.0	35.0	8.0	38.0
44	28.37520382622062	32.0	23.0	35.0	8.0	38.0
45	27.823473510380857	31.0	23.0	35.0	8.0	38.0
46	27.41369971853806	31.0	23.0	35.0	8.0	38.0
47	27.500314177996117	31.0	23.0	34.0	8.0	37.0
48	27.58424903669391	31.0	23.0	34.0	8.0	37.0
49	27.421042655505126	31.0	23.0	34.0	8.0	37.0
50	29.21876914927869	33.0	25.0	36.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	22.0
13	54.0
14	108.0
15	306.0
16	586.0
17	994.0
18	1793.0
19	2720.0
20	4193.0
21	5864.0
22	7682.0
23	10265.0
24	13273.0
25	16621.0
26	19507.0
27	22063.0
28	24038.0
29	28075.0
30	32970.0
31	38354.0
32	41444.0
33	40002.0
34	33757.0
35	25488.0
36	12398.0
37	2479.0
38	70.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.100744679746168	22.3845330951466	27.32699438114724	25.187727843959994
2	38.102001391730624	17.247333381801564	30.813850835557677	13.83681439091013
3	29.074966504990496	14.721186502290124	42.01053145415079	14.193315538568593
4	25.431280703758713	24.527694400880737	36.63055783471641	13.410467060644143
5	19.462937382507818	29.481580341285586	38.44084625530987	12.614636020896732
6	18.2911313523675	34.076887924140294	36.93305152519136	10.698929198300842
7	77.08629768494959	3.6753632520798067	17.540219976527528	1.6981190864430895
8	79.2021956108555	2.3677596252713355	16.59301226592441	1.8370324979487553
9	67.23902454223487	2.9935191051379784	16.747764402854088	13.019691949773065
10	47.40816135766438	20.66382435113156	22.8508667158273	9.077147575376754
11	41.82903523986582	17.99746580393216	29.182981419357517	10.99051753684451
12	36.76505717520227	19.287153495424946	31.95216185619476	11.995627473178027
13	20.431955796973504	34.10778642127894	32.786161627701674	12.674096154045886
14	14.219280662214514	35.247395698098316	37.10727750485548	13.426046134831694
15	12.632032653739497	18.382268936364675	52.200024926518694	16.785673483377128
16	14.876457941692719	15.01692926061714	49.08914346250117	21.017469335188974
17	15.756935284525825	16.072930839296657	37.49467714965259	30.67545672652493
18	20.751326817818306	19.36141374905227	41.71115357851335	18.17610585461608
19	27.125245370418455	20.754702283892275	34.208790752261564	17.911261593427707
20	27.73360821744233	21.209870901405232	33.652617803765985	17.403903077386452
21	20.50154232834457	24.93275032975707	36.158252235597146	18.407455106301214
22	19.93809914522813	20.84376265799778	34.62215552070459	24.595982676069504
23	16.91264293800567	25.88567036756229	34.37626579977774	22.8254208946543
24	17.613960927681937	20.411962651766146	46.03434666555882	15.939729754993092
25	16.124341784115575	21.336840356033775	42.94683381282262	19.59198404702803
26	12.361995367821942	40.79068994526552	29.522605236646136	17.3247094502664
27	15.233997694297019	30.232232065889097	36.69027761910203	17.84349262071186
28	14.18890146754879	24.625063614552932	44.49980785808502	16.68622705981326
29	15.127021384875835	21.729952328032986	43.49132245567753	19.651703831413645
30	18.286717281347695	26.291245598911544	38.19261967325488	17.229417446485883
31	27.730232751368362	22.625749093817184	33.03750402459416	16.606514130220287
32	27.79177009440919	22.088271034346665	33.6575511772587	16.46240769398544
33	26.628532555072027	23.625146702948598	31.624481995783267	18.121838746196108
34	19.153173457412002	23.70408067883219	34.24072785434604	22.902018009409762
35	19.520579957001754	24.754629581546066	34.98904271782142	20.735747743630757
36	27.985210265571286	23.49012805998982	30.10032923776783	18.424332436671065
37	19.155510318540138	31.396248558935635	30.175108793868077	19.273132328656146
38	20.72120727438904	31.029101710582346	27.538090836388562	20.71160017864005
39	21.202600666784374	28.532035769554337	28.77377107069784	21.491592492963452
40	24.182358256390017	23.866882004092105	28.893989593178443	23.056770146339435
41	18.080813850835558	21.925469709086755	30.144729599202353	29.848986840875334
42	21.152487978147754	22.366617159830916	27.674407735529638	28.806487126491696
43	21.29971022922011	23.190490533115916	29.55064757018373	25.959151667480242
44	19.139152290643207	26.853390525845683	30.763738146921053	23.24371903659005
45	16.902256888547303	34.03534372630683	26.705648972300406	22.356750412845468
46	20.70536854896503	28.6836720916465	28.82518201551676	21.78577734387171
47	20.621760850825172	25.179159353156837	29.721757735010335	24.477322061007655
48	21.224671021883406	23.25436473728488	33.49630775941755	22.024656481414166
49	20.749768910399553	22.640808865531817	33.52305183677285	23.086370387295784
50	18.362535442393778	29.936229656325626	30.15225948505967	21.54897541622093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	189.0
1	291.5
2	394.0
3	4434.5
4	8475.0
5	6608.0
6	4741.0
7	4634.0
8	4527.0
9	4877.0
10	5227.0
11	5479.0
12	5731.0
13	5628.0
14	5525.0
15	5346.5
16	5168.0
17	4879.5
18	4591.0
19	4300.0
20	4009.0
21	3815.5
22	3622.0
23	3506.5
24	3391.0
25	3172.0
26	2953.0
27	3093.5
28	3234.0
29	3721.5
30	4209.0
31	4562.5
32	4916.0
33	5823.5
34	6731.0
35	7601.5
36	8472.0
37	9150.5
38	9829.0
39	12736.0
40	15643.0
41	21023.5
42	26404.0
43	30700.5
44	34997.0
45	35390.0
46	35783.0
47	33560.0
48	31337.0
49	29259.0
50	27181.0
51	24949.0
52	22717.0
53	20689.0
54	18661.0
55	17212.5
56	15764.0
57	14874.0
58	13984.0
59	12985.0
60	11986.0
61	10827.0
62	9668.0
63	8611.5
64	7555.0
65	6597.0
66	5639.0
67	4774.5
68	3910.0
69	3394.0
70	2878.0
71	2459.5
72	2041.0
73	1660.5
74	1280.0
75	1048.0
76	816.0
77	611.5
78	407.0
79	327.0
80	247.0
81	180.0
82	113.0
83	96.0
84	79.0
85	59.5
86	40.0
87	37.0
88	34.0
89	24.5
90	15.0
91	13.5
92	12.0
93	9.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	385132.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.95474910633825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17968840663103	42.69625092803569
2	7.073914271743896	7.350468823763991
3	2.8802062780050726	4.489211836447616
4	1.5890991571304554	3.3024499205522555
5	1.1345736021993296	2.947324342247029
6	0.7476787599148386	2.330727743010813
7	0.6208166208890542	2.257806024553487
8	0.49569797018654954	2.0603090938850652
9	0.37396360324104816	1.748626666316179
>10	2.841151944745824	26.88749944651882
>50	0.05834712490423696	1.947400390160294
>100	0.003781758095644985	0.30594788487672514
>500	5.402511565207121E-4	0.2674938846674487
>1k	0.0	0.0
>5k	5.402511565207121E-4	1.4084830149645935
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5018	1.3029299045522054	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	953	0.24744762834560619	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03011954342926581	0.0
2	0.0	0.0	0.0	0.13657655037753288	0.0
3	0.0	0.0	0.0	0.1908436587975032	0.0
4	0.0	0.0	0.0	0.27315310075506577	0.0
5	0.0	0.0	0.0	0.4998286301839369	0.0
6	0.0	0.0	0.0	0.680805541995991	0.0
7	0.0	0.0	0.0	0.8093329040432891	0.0
8	0.0	0.0	0.0	1.1837499870174382	0.0
9	0.0	0.0	0.0	1.403155281825452	0.0
10	0.0	0.0	0.0	1.7451159602422026	0.0
11	0.0	0.0	0.0	1.980100329237768	0.0
12	0.0	0.0	0.0	2.2088530685583123	0.0
13	0.0	0.0	0.0	2.302067862447161	0.0
14	0.0	0.0	0.0	2.3342646157681	0.0
15	0.0	0.0	0.0	2.383598350695346	0.0
16	0.0	0.0	0.0	2.4726587248008474	0.0
17	0.0	0.0	0.0	2.599108876956472	0.0
18	0.0	0.0	0.0	2.764766365817434	0.0
19	0.0	0.0	0.0	2.8250054526759656	0.0
20	0.0	0.0	0.0	2.9026411723772627	0.0
21	0.0	0.0	0.0	2.975603169822295	0.0
22	0.0	0.0	0.0	3.0527195870506736	0.0
23	0.0	0.0	0.0	3.137365890136369	0.0
24	0.0	0.0	0.0	3.2059138165615946	0.0
25	0.0	0.0	0.0	3.2599212737451055	0.0
26	0.0	0.0	0.0	3.3204200118400964	0.0
27	0.0	0.0	0.0	3.398575034014312	0.0
28	0.0	0.0	0.0	3.4668633092030783	0.0
29	0.0	0.0	0.0	3.550730658579396	0.0
30	0.0	0.0	0.0	3.652513943271398	0.0
31	0.0	0.0	0.0	3.7389778050123073	0.0
32	0.0	0.0	0.0	3.815834571004227	0.0
33	0.0	0.0	0.0	3.905154596346188	0.0
34	0.0	0.0	0.0	3.986685084594373	0.0
35	0.0	0.0	0.0	4.103787792237466	0.0
36	0.0	0.0	0.0	4.189732351505458	0.0
37	0.0	0.0	0.0	4.282947145394306	0.0
38	0.0	0.0	0.0	4.409397297549931	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATC	20	7.849954E-4	44.000004	22
ACGTTAG	20	7.849954E-4	44.000004	1
CCTAACG	20	7.849954E-4	44.000004	1
GCGTTAG	20	7.849954E-4	44.000004	1
TCGTGAT	20	7.849954E-4	44.000004	28
GGAACCT	20	7.849954E-4	44.000004	8
GGTGCGA	20	7.849954E-4	44.000004	41
AGTACGG	20	7.849954E-4	44.000004	2
ACGACGG	40	8.287316E-9	44.000004	27
TCACGAC	35	1.4427314E-7	44.0	25
CTCACGA	35	1.4427314E-7	44.0	24
CGTGCGG	45	4.783942E-10	44.0	2
AGGCGGT	35	1.4427314E-7	44.0	6
CGACAGG	25	4.4373097E-5	44.0	2
ATCGTAG	35	1.4427314E-7	44.0	1
ATCCGGT	25	4.4373097E-5	44.0	26
GTCCGGT	25	4.4373097E-5	44.0	26
ATAGTCT	25	4.4373097E-5	44.0	24
TACCGGG	55	1.8189894E-12	44.0	3
TAATCGT	25	4.4373097E-5	44.0	21
>>END_MODULE