FastQCFastQC Report
Thu 26 May 2016
SRR1545965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545965_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences931663
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165741.7789694342267535No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12950.1389987581346474No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG207.8557123E-444.0000041
ATGTACG207.8557123E-444.0000041
GCGATAC207.8557123E-444.0000049
ACTCGAA408.309144E-944.00000412
ACGTTAG454.802132E-1044.01
CCGCTCG351.4456964E-744.019
TAGCACG351.4456964E-744.01
GTATACT302.5271984E-644.020
TATAGCG351.4456964E-744.01
ACCGTTA254.442189E-544.044
TCGAACG502.7284841E-1144.01
CGTTTTT127800.043.1909261
TTCGTAG1000.041.81
GTTAGCG603.6379788E-1240.3333321
GCGATAT603.6379788E-1240.3333329
ACGGTCT1200.040.33333230
CGGGATT1600.039.8750046
GTTTTTT144100.039.175572
TAACGCC452.3501343E-839.1111112
CTACGAT452.3501343E-839.1111143
Produced by FastQC (version 0.11.5)