##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545965_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	931663
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.232637767089603	31.0	28.0	33.0	25.0	33.0
2	28.75516254267906	31.0	28.0	33.0	16.0	33.0
3	28.1755495281019	31.0	27.0	33.0	16.0	34.0
4	32.128537894066845	35.0	32.0	35.0	25.0	37.0
5	33.62930265557396	35.0	33.0	35.0	28.0	37.0
6	33.48557042621635	35.0	33.0	37.0	28.0	37.0
7	34.31777906818238	35.0	35.0	37.0	31.0	37.0
8	34.728803226059206	35.0	35.0	37.0	32.0	37.0
9	36.514891114061626	39.0	37.0	39.0	32.0	39.0
10	36.337747661976486	38.0	35.0	39.0	32.0	39.0
11	36.33599166222121	38.0	35.0	39.0	32.0	39.0
12	35.04920663372915	37.0	34.0	39.0	29.0	39.0
13	34.97680062426006	37.0	34.0	39.0	27.0	39.0
14	35.723565280578924	38.0	33.0	40.0	27.0	41.0
15	36.40654399713201	38.0	35.0	40.0	31.0	41.0
16	36.07728652957132	37.0	34.0	40.0	31.0	41.0
17	35.66210743584322	37.0	34.0	40.0	29.0	41.0
18	35.288917773916104	37.0	33.0	39.0	29.0	40.0
19	34.70418380895238	36.0	33.0	39.0	27.0	40.0
20	35.105003633288	35.0	34.0	39.0	29.0	40.0
21	35.27746406157591	35.0	34.0	39.0	30.0	40.0
22	35.626448619296895	35.0	34.0	39.0	31.0	40.0
23	35.97708613522271	36.0	35.0	40.0	31.0	41.0
24	35.64839754288836	35.0	34.0	40.0	31.0	41.0
25	35.28872671770801	35.0	34.0	39.0	30.0	41.0
26	35.3578804782416	35.0	34.0	40.0	30.0	41.0
27	35.630088347396	35.0	34.0	40.0	31.0	41.0
28	35.5746756069523	36.0	35.0	40.0	31.0	41.0
29	35.7403685667457	36.0	35.0	40.0	31.0	41.0
30	35.37446802116216	36.0	34.0	40.0	30.0	41.0
31	34.683750454831845	35.0	34.0	39.0	29.0	41.0
32	34.544147400937895	35.0	34.0	40.0	27.0	41.0
33	34.18626584934681	35.0	33.0	40.0	23.0	41.0
34	33.809611415286426	35.0	33.0	40.0	21.0	41.0
35	33.487117122822305	35.0	33.0	40.0	18.0	41.0
36	33.041593365841514	35.0	33.0	40.0	16.0	41.0
37	32.91129410527197	35.0	33.0	40.0	15.0	41.0
38	32.78521418152272	35.0	33.0	40.0	15.0	41.0
39	32.58628173491917	35.0	32.0	39.0	15.0	40.0
40	32.42058340837835	35.0	32.0	39.0	15.0	40.0
41	32.13993901228234	35.0	31.0	39.0	12.0	40.0
42	32.30908493736469	35.0	32.0	39.0	12.0	40.0
43	32.08885723700523	35.0	31.0	39.0	10.0	40.0
44	31.796619593136146	35.0	31.0	39.0	10.0	40.0
45	31.777699661787576	35.0	31.0	39.0	10.0	40.0
46	31.651724926287724	35.0	31.0	38.0	10.0	40.0
47	31.658023341057874	35.0	31.0	38.0	10.0	40.0
48	31.70953123608	35.0	31.0	38.0	10.0	40.0
49	31.724032187604315	35.0	31.0	38.0	10.0	40.0
50	31.824509506119703	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	6.0
13	22.0
14	43.0
15	117.0
16	256.0
17	590.0
18	1139.0
19	1954.0
20	3039.0
21	4576.0
22	6587.0
23	9209.0
24	13174.0
25	19797.0
26	28228.0
27	31881.0
28	29932.0
29	28899.0
30	30643.0
31	37322.0
32	50122.0
33	77025.0
34	123794.0
35	86091.0
36	82713.0
37	113468.0
38	124002.0
39	27028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.574121758618727	22.687602706128718	27.606334049973004	25.131941485279548
2	36.79957237756571	21.184054749410464	29.48780835988979	12.528564513134041
3	26.63312807313374	19.097785358010352	41.308284218649874	12.96080235020603
4	23.966605950864206	25.100170340563054	37.62852018380037	13.304703524772368
5	20.371207185430784	28.61775126843075	38.1971807402462	12.81386080589226
6	18.708159495439876	35.55051558342448	35.29613175579582	10.445193165339827
7	74.58426491123936	4.907997849007635	17.757064517964114	2.750672721788887
8	75.90512878583779	3.512321515397735	17.497957952607326	3.0845917461571406
9	72.3609287907752	4.9424523674332885	18.987230361192832	3.7093884805986717
10	45.378962135450266	21.41385887386319	23.157622445025723	10.049556545660824
11	39.98098024714945	18.2172094416114	30.12591462792877	11.675895683310381
12	36.17370229364052	19.162293661978634	32.115475230850635	12.548528813530213
13	19.295281663004758	35.815632905889785	31.865921475898475	13.023163955206979
14	13.838802227844187	36.07956954392307	35.90193020437648	14.179698023856266
15	12.47360901957038	21.088633980312625	52.62836454812524	13.809392451991762
16	14.40553075521943	17.1424646036174	50.636442576339306	17.81556206482387
17	14.948108919212205	17.451374585016254	36.92408091767088	30.676435578100662
18	19.735032946462404	20.48283553173197	41.41572650196477	18.366405019840865
19	24.800383829775356	22.331143342603497	33.82789699709015	19.040575830530997
20	26.788763748265197	20.53983038931459	34.12854218746478	18.542863674955427
21	19.363976029959332	25.546361720922693	36.104471251944105	18.98519099717387
22	20.091814314832725	22.30269958128637	33.88006178199628	23.72542432188463
23	16.65387591865299	27.010517751590434	33.84700261789939	22.488603711857184
24	17.050049213073827	21.183947414462097	45.4759929287736	16.290010443690477
25	15.556912746347123	22.61837166443231	42.19079216411943	19.633923425101134
26	14.66023658769319	30.032211218004797	35.85266346307624	19.454888731225775
27	15.37336998464037	29.790385579335016	36.89155842831582	17.944686007708796
28	13.850072397422672	24.9929427271449	44.77316368686961	16.383821188562816
29	14.797410651705606	21.04709535529478	43.56006410043116	20.59542989256845
30	17.082142362635416	26.867547600366226	38.2023328177678	17.84797721923056
31	25.204285240478587	23.84456611457147	32.788250687212006	18.162897957737936
32	25.949941126780825	23.178874764802295	33.56181366008954	17.309370448327346
33	24.427931558943524	24.70979313335401	31.621412463519533	19.240862844182928
34	18.1688013798981	24.67372859070286	34.26389155735497	22.893578472044076
35	19.10615748398294	23.945675635932737	35.76722484417649	21.180942035907833
36	27.204042663495276	23.638483013707745	30.299582574385802	18.857891748411173
37	18.847587593367987	29.502191242970905	33.194835471624394	18.455385692036714
38	20.35038420544768	29.475250170930906	29.437682939002624	20.736682684618795
39	20.190455132381558	28.455460826500566	31.28803011389311	20.066053927224758
40	23.002845449481196	24.829578935731053	29.76237115781136	22.405204456976396
41	17.694917582859897	22.81168190644042	31.865062796311545	27.62833771438814
42	21.462374270524855	23.512042444531982	28.379253013160337	26.646330271782826
43	20.970028862367617	25.309902829671245	29.12254753059851	24.59752077736263
44	19.576499227725048	28.252383104191107	30.280262283679825	21.890855384404016
45	16.962141890361643	34.15655660898844	26.918317030943594	21.96298446970632
46	20.895538408201247	29.591386585063482	29.17342429612424	20.339650710611025
47	20.080758815150972	25.778097874446015	30.899906940599763	23.241236369803246
48	21.52151582707481	23.0246344439996	33.72045471377526	21.73339501515033
49	19.870704321197685	23.118123184026842	34.1932651613298	22.817907333445678
50	18.44443752730333	29.76687922564275	30.944987618913704	20.843695628140217
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	506.0
1	784.0
2	1062.0
3	13291.5
4	25521.0
5	18404.5
6	11288.0
7	11727.5
8	12167.0
9	12915.5
10	13664.0
11	13564.0
12	13464.0
13	12890.0
14	12316.0
15	11476.5
16	10637.0
17	9942.0
18	9247.0
19	8382.5
20	7518.0
21	7118.0
22	6718.0
23	6928.5
24	7139.0
25	6837.0
26	6535.0
27	7759.0
28	8983.0
29	9999.5
30	11016.0
31	13037.5
32	15059.0
33	17373.5
34	19688.0
35	22498.5
36	25309.0
37	25986.0
38	26663.0
39	33501.5
40	40340.0
41	53490.5
42	66641.0
43	77426.5
44	88212.0
45	88464.0
46	88716.0
47	82233.5
48	75751.0
49	74085.0
50	72419.0
51	69242.0
52	66065.0
53	57179.0
54	48293.0
55	42137.5
56	35982.0
57	31328.5
58	26675.0
59	24255.0
60	21835.0
61	19090.0
62	16345.0
63	14891.5
64	13438.0
65	11102.5
66	8767.0
67	7461.5
68	6156.0
69	5131.5
70	4107.0
71	3509.0
72	2911.0
73	2507.0
74	2103.0
75	1624.0
76	1145.0
77	896.5
78	648.0
79	490.0
80	332.0
81	236.0
82	140.0
83	103.0
84	66.0
85	46.5
86	27.0
87	20.5
88	14.0
89	13.0
90	12.0
91	13.5
92	15.0
93	10.0
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	931663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.771299993061245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.32492878217556	22.24738722934069
2	7.058347312124525	4.061556559447044
3	3.135578944886623	2.706440474257785
4	2.031856144669785	2.33836570724157
5	1.428416152179467	2.0548694814644834
6	1.0918023526466263	1.8847543812675678
7	0.792256900195655	1.5955982662971384
8	0.6284000341890715	1.4463908719442975
9	0.5372636706045087	1.3911996818102004
>10	4.766602541389191	30.36707055489626
>50	0.9923996768728658	19.858391099482052
>100	0.21139652350656118	8.117567374626091
>500	0.0	0.0
>1k	3.754822797629856E-4	0.13990031740515532
>5k	0.0	0.0
>10k+	3.754822797629856E-4	1.7905080005197256
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16574	1.7789694342267535	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1295	0.1389987581346474	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.054848158615293297	0.0
2	0.0	0.0	0.0	0.1891241790218137	0.0
3	0.0	0.0	0.0	0.25041243453909834	0.0
4	0.0	0.0	0.0	0.3737402902122334	0.0
5	0.0	0.0	0.0	0.6517378064815282	0.0
6	0.0	0.0	0.0	0.948411603766598	0.0
7	0.0	0.0	0.0	1.1744590050264956	0.0
8	1.0733494836652309E-4	0.0	0.0	1.7305613725134517	0.0
9	1.0733494836652309E-4	0.0	0.0	2.035714630719477	0.0
10	2.1466989673304618E-4	0.0	0.0	2.4994016076628567	0.0
11	3.2200484509956924E-4	0.0	0.0	2.791460002168166	0.0
12	4.2933979346609235E-4	0.0	0.0	3.098437954496422	0.0
13	5.366747418326154E-4	0.0	0.0	3.2110323153329046	0.0
14	5.366747418326154E-4	0.0	0.0	3.2508535811768846	0.0
15	5.366747418326154E-4	0.0	0.0	3.3015156768058835	0.0
16	5.366747418326154E-4	0.0	0.0	3.4161494016613303	0.0
17	7.513446385656616E-4	0.0	0.0	3.5528941258802806	0.0
18	7.513446385656616E-4	0.0	0.0	3.7712134108577886	0.0
19	7.513446385656616E-4	0.0	0.0	3.846777214507821	0.0
20	7.513446385656616E-4	0.0	0.0	3.9394072749481306	0.0
21	7.513446385656616E-4	0.0	0.0	4.049210927127083	0.0
22	7.513446385656616E-4	0.0	0.0	4.161053943325	0.0
23	7.513446385656616E-4	0.0	0.0	4.274292313851682	0.0
24	7.513446385656616E-4	0.0	0.0	4.361662961822033	0.0
25	7.513446385656616E-4	0.0	0.0	4.434221386917802	0.0
26	7.513446385656616E-4	0.0	0.0	4.513541913760663	0.0
27	7.513446385656616E-4	0.0	0.0	4.593721120190455	0.0
28	9.660145352987078E-4	0.0	0.0	4.670036268479053	0.0
29	9.660145352987078E-4	0.0	0.0	4.75751425139777	0.0
30	9.660145352987078E-4	0.0	0.0	4.88899956314676	0.0
31	9.660145352987078E-4	0.0	0.0	4.987533045747228	0.0
32	9.660145352987078E-4	0.0	0.0	5.074796358769212	0.0
33	0.0010733494836652308	0.0	0.0	5.163240356223227	0.0
34	0.0010733494836652308	0.0	0.0	5.256299756457002	0.0
35	0.0010733494836652308	0.0	0.0	5.3843503498582646	0.0
36	0.0010733494836652308	0.0	0.0	5.475263051124709	0.0
37	0.0010733494836652308	0.0	0.0	5.572723184241513	0.0
38	0.0010733494836652308	0.0	0.0	5.672115346428913	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	20	7.8557123E-4	44.000004	1
ATGTACG	20	7.8557123E-4	44.000004	1
GCGATAC	20	7.8557123E-4	44.000004	9
ACTCGAA	40	8.309144E-9	44.000004	12
ACGTTAG	45	4.802132E-10	44.0	1
CCGCTCG	35	1.4456964E-7	44.0	19
TAGCACG	35	1.4456964E-7	44.0	1
GTATACT	30	2.5271984E-6	44.0	20
TATAGCG	35	1.4456964E-7	44.0	1
ACCGTTA	25	4.442189E-5	44.0	44
TCGAACG	50	2.7284841E-11	44.0	1
CGTTTTT	12780	0.0	43.190926	1
TTCGTAG	100	0.0	41.8	1
GTTAGCG	60	3.6379788E-12	40.333332	1
GCGATAT	60	3.6379788E-12	40.333332	9
ACGGTCT	120	0.0	40.333332	30
CGGGATT	160	0.0	39.875004	6
GTTTTTT	14410	0.0	39.17557	2
TAACGCC	45	2.3501343E-8	39.11111	12
CTACGAT	45	2.3501343E-8	39.11111	43
>>END_MODULE