FastQCFastQC Report
Thu 26 May 2016
SRR1545964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545964_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences937462
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167971.7917526257064287No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12920.13781891959354087No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG302.5272147E-644.0000041
TTACGCC302.5272147E-644.00000418
GCGTAAG600.044.0000041
CTTACGC302.5272147E-644.00000417
CTAGACG351.4456964E-744.01
TACCCGC207.8557397E-444.017
GCGAAAA207.8557397E-444.09
CCGTCTA207.8557397E-444.044
TTACGAT207.8557397E-444.02
TAATGCG207.8557397E-444.01
TCGAACG207.8557397E-444.01
TACGTAG800.044.01
CGTTTTT126550.043.2003171
TACGGGA1250.042.244
CGTAAGG1500.041.066672
TCGTAAG700.040.8571431
ACGTTAG650.040.6153831
GCGTTAG650.040.6153831
ATGGGAC3850.040.05
TAGAACG557.8216544E-1140.01
Produced by FastQC (version 0.11.5)