##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545964_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	937462
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.209115676155406	31.0	28.0	33.0	25.0	33.0
2	28.72544700478526	31.0	28.0	33.0	16.0	33.0
3	28.16604619707252	31.0	27.0	33.0	16.0	34.0
4	32.1065963207042	35.0	32.0	35.0	25.0	37.0
5	33.664649873808216	35.0	33.0	35.0	28.0	37.0
6	33.581382498703945	35.0	33.0	37.0	28.0	37.0
7	34.37627551836768	35.0	35.0	37.0	31.0	37.0
8	34.755483422261385	35.0	35.0	37.0	32.0	37.0
9	36.60942736878935	39.0	37.0	39.0	32.0	39.0
10	36.32335710674139	39.0	35.0	39.0	32.0	39.0
11	36.38005593826736	39.0	35.0	39.0	32.0	39.0
12	34.983544933021285	37.0	34.0	39.0	29.0	39.0
13	34.985607949975574	37.0	34.0	39.0	27.0	39.0
14	35.74454964574564	38.0	33.0	40.0	27.0	41.0
15	36.44712532347978	38.0	35.0	40.0	31.0	41.0
16	36.10970471336438	37.0	34.0	40.0	31.0	41.0
17	35.70103854876251	37.0	34.0	40.0	30.0	41.0
18	35.374726655587104	37.0	34.0	39.0	29.0	40.0
19	34.781320202845556	36.0	33.0	39.0	27.0	40.0
20	35.06761874081296	35.0	34.0	39.0	29.0	40.0
21	35.179431272947596	35.0	34.0	39.0	30.0	40.0
22	35.592291740891895	35.0	34.0	39.0	31.0	40.0
23	35.985358339858045	36.0	35.0	40.0	31.0	41.0
24	35.555033697365864	35.0	34.0	40.0	31.0	41.0
25	35.20742494095761	35.0	34.0	39.0	30.0	41.0
26	35.32150316492829	35.0	34.0	39.0	30.0	41.0
27	35.655773780697245	35.0	34.0	40.0	31.0	41.0
28	35.59600922490725	36.0	35.0	40.0	31.0	41.0
29	35.751925944731624	36.0	35.0	40.0	31.0	41.0
30	35.417608393726894	36.0	34.0	40.0	30.0	41.0
31	34.71969637169293	35.0	34.0	39.0	29.0	41.0
32	34.61136238055516	35.0	34.0	40.0	27.0	41.0
33	34.271516072118125	35.0	34.0	40.0	24.0	41.0
34	33.82345204392285	35.0	34.0	40.0	21.0	41.0
35	33.53432992483962	35.0	33.0	40.0	18.0	41.0
36	33.06255080205918	35.0	33.0	40.0	16.0	41.0
37	32.92465827948226	35.0	33.0	40.0	15.0	41.0
38	32.84369073093096	35.0	33.0	40.0	15.0	41.0
39	32.60984125223209	35.0	32.0	39.0	15.0	40.0
40	32.41853323121364	35.0	32.0	39.0	15.0	40.0
41	32.12915936859307	35.0	31.0	39.0	12.0	40.0
42	32.30664816280553	35.0	32.0	39.0	12.0	40.0
43	32.093509923602234	35.0	31.0	39.0	10.0	40.0
44	31.811481425380443	35.0	31.0	39.0	10.0	40.0
45	31.786819092400545	35.0	31.0	39.0	10.0	40.0
46	31.63806532958136	35.0	31.0	38.0	10.0	40.0
47	31.647800124165034	35.0	31.0	38.0	10.0	40.0
48	31.685658725367002	35.0	31.0	38.0	10.0	40.0
49	31.75628238797946	35.0	31.0	38.0	10.0	40.0
50	31.875424283864305	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	8.0
13	17.0
14	39.0
15	91.0
16	261.0
17	623.0
18	1060.0
19	1995.0
20	3140.0
21	4408.0
22	6353.0
23	9148.0
24	13045.0
25	19958.0
26	28281.0
27	31519.0
28	30423.0
29	29093.0
30	30791.0
31	36910.0
32	49795.0
33	77828.0
34	125737.0
35	86250.0
36	84260.0
37	116900.0
38	125146.0
39	24380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.66681316149348	22.625450418256953	27.47748708747661	25.230249332772953
2	36.97248528473687	21.129603119913128	29.372603902878197	12.525307692471802
3	26.613025381295458	19.072986425049763	41.23132457635616	13.082663617298621
4	23.892808455169384	25.042401718683	37.65965980487743	13.405130021270196
5	20.35943856924334	28.601692655275627	38.20165510708701	12.837213668394027
6	18.765133946762642	35.62203054630481	35.27374976265705	10.339085744275502
7	74.45976476913198	4.9333199639025365	17.8238691274953	2.7830461394701866
8	75.68893459148211	3.5992925579916837	17.583859399100977	3.1279134514252314
9	72.28730337869695	4.967988035781716	19.10264096038026	3.6420676251410726
10	45.292289180788124	21.257074953438114	23.199767030556973	10.250868835216789
11	39.97836712314739	18.176203408778168	30.10607363285125	11.739355835223188
12	36.14781185797398	18.853990881763742	32.10572801884236	12.892469241419919
13	19.302009041433145	35.7959042606527	31.9435881134382	12.958498584475958
14	13.928777913131412	36.015113145919514	35.883481143769025	14.172627797180045
15	12.544295128762553	21.045973063441505	52.68480215731412	13.724929650481831
16	14.417864404103847	17.233338524654865	50.568129694856964	17.780667376384322
17	14.978207116661796	17.554098192780078	36.86816105612814	30.59953363442998
18	19.746933742381025	20.564353541796894	41.37799718815269	18.310715527669387
19	24.721962063528974	22.332745220606277	33.89086704314415	19.0544256727206
20	26.74551075136912	20.395920047959276	34.19626608865213	18.662303112019472
21	19.440147973997878	25.376495260607896	36.220774815405846	18.962581949988373
22	20.07910720647877	22.27204942707011	33.91028116339649	23.73856220305463
23	16.71641090518869	26.943705451527634	33.906867691703766	22.433015951579904
24	17.077385536693754	21.164164520801908	45.45261567935553	16.3058342631488
25	15.599885648698294	22.60411622017746	42.13621458789796	19.659783543226286
26	14.611258909694472	29.960681072939487	36.02610025793045	19.401959759435584
27	15.445319383612349	29.676296212539814	36.95691132013884	17.921473083708996
28	13.873522340105518	24.971465510068676	44.73237315219177	16.42263899763404
29	14.771478737271485	21.017918592966968	43.536378007855255	20.674224661906297
30	17.106613388062662	26.7590579671496	38.28389844068346	17.850430204104274
31	25.11589803106686	23.838512921057067	32.88687968152309	18.158709366352983
32	25.871021972090603	23.16424559075461	33.63741676995974	17.32731566719504
33	24.353947146657678	24.850927290919525	31.72021905954588	19.074906502876917
34	18.120947835752276	24.703721324171006	34.37152652587518	22.803804314201535
35	19.09752075284118	24.035747582302	35.72251461925923	21.144217045597582
36	27.17859497238288	23.56554185662992	30.345123322331997	18.910739848655197
37	18.770787509253708	29.474261356726995	33.34204479754913	18.412906336470172
38	20.425681254280175	29.410792117440494	29.38764451252424	20.775882115755092
39	20.216712784091513	28.35464264151507	31.42271366732732	20.005930907066098
40	22.916342209070876	24.85572748548741	29.774753536676684	22.453176768765026
41	17.60860706887319	22.968610994365637	31.944548152351775	27.478233784409394
42	21.572074388081862	23.562021713946805	28.40563137492506	26.460272523046267
43	20.982930508116596	25.485192999822925	29.05237758970497	24.47949890235551
44	19.558766115319877	28.34365552950413	30.21349132018151	21.884087034994483
45	16.997915648847634	34.06484742848244	27.029362256816807	21.90787466585312
46	20.991037503386803	29.554157928534703	29.185929669682608	20.26887489839588
47	20.154950280651377	25.78387177293586	30.891918819109463	23.16925912730329
48	21.689199135538296	23.05725458738594	33.555066765372885	21.698479511702875
49	19.93488802746138	23.25736936537161	33.9861242375691	22.821618369597914
50	18.427200249183436	29.681416420078893	31.07176610891961	20.819617221818056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	504.0
1	789.5
2	1075.0
3	13480.0
4	25885.0
5	18681.5
6	11478.0
7	11945.5
8	12413.0
9	13170.0
10	13927.0
11	13838.0
12	13749.0
13	13001.0
14	12253.0
15	11372.0
16	10491.0
17	9847.0
18	9203.0
19	8443.5
20	7684.0
21	7219.5
22	6755.0
23	6947.5
24	7140.0
25	6884.0
26	6628.0
27	7798.5
28	8969.0
29	10039.5
30	11110.0
31	13044.5
32	14979.0
33	17585.0
34	20191.0
35	22832.0
36	25473.0
37	26158.5
38	26844.0
39	33594.0
40	40344.0
41	53655.5
42	66967.0
43	77434.0
44	87901.0
45	88424.0
46	88947.0
47	82352.0
48	75757.0
49	74337.0
50	72917.0
51	69778.0
52	66639.0
53	57738.0
54	48837.0
55	42422.5
56	36008.0
57	31651.5
58	27295.0
59	24540.0
60	21785.0
61	19206.5
62	16628.0
63	15185.5
64	13743.0
65	11357.5
66	8972.0
67	7636.5
68	6301.0
69	5218.5
70	4136.0
71	3564.0
72	2992.0
73	2574.5
74	2157.0
75	1648.5
76	1140.0
77	878.0
78	616.0
79	482.5
80	349.0
81	252.0
82	155.0
83	108.0
84	61.0
85	42.0
86	23.0
87	18.5
88	14.0
89	11.0
90	8.0
91	12.0
92	16.0
93	9.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	937462.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.583556465547833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86304297585681	21.970191290142328
2	7.2743480515803	4.158534765647866
3	3.225903514088028	2.7662338574203296
4	2.0416463886952214	2.334300593358067
5	1.4522706848332612	2.0755530561595665
6	1.0704128754985445	1.835772412095724
7	0.8349720282478765	1.6706529081603325
8	0.6760995844858027	1.546026451958669
9	0.5371769159190691	1.3818984037345396
>10	4.814648343076012	30.362184642833828
>50	0.9928949535577636	19.773736404743808
>100	0.21583034960773115	8.177364769774437
>500	0.0	0.0
>1k	3.766672768023225E-4	0.13910305564762687
>5k	0.0	0.0
>10k+	3.766672768023225E-4	1.8084473883229015
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16797	1.7917526257064287	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1292	0.13781891959354087	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.051095404400391696	0.0
2	1.0667099039747744E-4	0.0	0.0	0.1872075881475729	0.0
3	1.0667099039747744E-4	0.0	0.0	0.2483300656453275	0.0
4	1.0667099039747744E-4	0.0	0.0	0.3654548131017577	0.0
5	1.0667099039747744E-4	0.0	0.0	0.6417326782312243	0.0
6	1.0667099039747744E-4	0.0	0.0	0.939024728468994	0.0
7	1.0667099039747744E-4	0.0	0.0	1.1520466962927565	0.0
8	1.0667099039747744E-4	0.0	0.0	1.71473617063945	0.0
9	1.0667099039747744E-4	0.0	0.0	2.0028545157030364	0.0
10	1.0667099039747744E-4	0.0	0.0	2.475727016135054	0.0
11	1.0667099039747744E-4	0.0	0.0	2.7497647904661737	0.0
12	1.0667099039747744E-4	0.0	0.0	3.0549504939933567	0.0
13	2.1334198079495488E-4	0.0	0.0	3.1641815881603734	0.0
14	2.1334198079495488E-4	0.0	0.0	3.1994896859819386	0.0
15	2.1334198079495488E-4	0.0	0.0	3.2527185101902796	0.0
16	3.2001297119243235E-4	0.0	0.0	3.357362751770205	0.0
17	3.2001297119243235E-4	0.0	0.0	3.4937949484885786	0.0
18	3.2001297119243235E-4	0.0	0.0	3.71321717573619	0.0
19	4.2668396158990977E-4	0.0	0.0	3.7920470376399256	0.0
20	5.333549519873872E-4	0.0	0.0	3.8918910846519648	0.0
21	5.333549519873872E-4	0.0	0.0	4.001975546742162	0.0
22	6.400259423848647E-4	0.0	0.0	4.112700034774742	0.0
23	6.400259423848647E-4	0.0	0.0	4.225451271624877	0.0
24	6.400259423848647E-4	0.0	0.0	4.318255033270682	0.0
25	6.400259423848647E-4	0.0	0.0	4.390577964760172	0.0
26	6.400259423848647E-4	0.0	0.0	4.464714303086418	0.0
27	6.400259423848647E-4	0.0	0.0	4.545250900836514	0.0
28	7.466969327823421E-4	0.0	0.0	4.622587368874685	0.0
29	7.466969327823421E-4	0.0	0.0	4.7100575810006164	0.0
30	7.466969327823421E-4	0.0	0.0	4.8427562930550785	0.0
31	8.533679231798195E-4	0.0	0.0	4.9363067516336665	0.0
32	8.533679231798195E-4	0.0	0.0	5.0236702927692	0.0
33	8.533679231798195E-4	0.0	0.0	5.10655365230804	0.0
34	8.533679231798195E-4	0.0	0.0	5.203517582579347	0.0
35	8.533679231798195E-4	0.0	0.0	5.3323761389795	0.0
36	8.533679231798195E-4	0.0	0.0	5.42709997845246	0.0
37	8.533679231798195E-4	0.0	0.0	5.522357172877407	0.0
38	8.533679231798195E-4	0.0	0.0	5.627641440399717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	30	2.5272147E-6	44.000004	1
TTACGCC	30	2.5272147E-6	44.000004	18
GCGTAAG	60	0.0	44.000004	1
CTTACGC	30	2.5272147E-6	44.000004	17
CTAGACG	35	1.4456964E-7	44.0	1
TACCCGC	20	7.8557397E-4	44.0	17
GCGAAAA	20	7.8557397E-4	44.0	9
CCGTCTA	20	7.8557397E-4	44.0	44
TTACGAT	20	7.8557397E-4	44.0	2
TAATGCG	20	7.8557397E-4	44.0	1
TCGAACG	20	7.8557397E-4	44.0	1
TACGTAG	80	0.0	44.0	1
CGTTTTT	12655	0.0	43.200317	1
TACGGGA	125	0.0	42.24	4
CGTAAGG	150	0.0	41.06667	2
TCGTAAG	70	0.0	40.857143	1
ACGTTAG	65	0.0	40.615383	1
GCGTTAG	65	0.0	40.615383	1
ATGGGAC	385	0.0	40.0	5
TAGAACG	55	7.8216544E-11	40.0	1
>>END_MODULE